New insights into the fructosyltransferase activity of schwanniomyces occidentalis β-fructofuranosidase, emerging from nonconventional codon usage and directed mutation

Applied and Environmental Microbiology

Volumn 76, Issue 22, 2010, Pages 7491-7499

  Á lvaro Benito, Miguel ^a   De Abreu, Miguel ^a   Portillo, Francisco ^b   Sanz Aparicio, Julia ^c   Fernández Lobato, María ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b INSTITUTO DE INVESTIGACIONES BIOMÉDICAS ALBERTO SOLS   (Spain)

^c INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; AMINO ACID SEQUENCE; CATALYTIC EFFICIENCIES; CODON USAGE; DIRECTED MUTATION; FRUCTANS; FRUCTO-OLIGOSACCHARIDES; FRUCTOSYLTRANSFERASE; KESTOSE; PREBIOTICS; SCHWANNIOMYCES OCCIDENTALIS; TRANSFRUCTOSYLATION REACTIONS; WILD-TYPE ENZYMES;

AMINO ACIDS; ENZYMES;

ENZYME ACTIVITY;

1 KESTOSE; 1-KESTOSE; 6 KESTOSE; 6-KESTOSE; BETA FRUCTOFURANOSIDASE; FRUCTAN; FUNGAL DNA; TRISACCHARIDE;

AMINO ACID; ENZYME ACTIVITY; MOLECULAR ANALYSIS; MUTATION; PROTEIN; REACTION KINETICS; YEAST;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; CODON; DNA SEQUENCE; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MISSENSE MUTATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SACCHAROMYCETALES; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BETA-FRUCTOFURANOSIDASE; CODON; DNA, FUNGAL; FRUCTANS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; SACCHAROMYCETALES; SEQUENCE ANALYSIS, DNA; TRISACCHARIDES;

DEBARYOMYCES OCCIDENTALIS;

EID: 78649710607     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01614-10     Document Type: Article

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