High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis

Journal of Microbiological Methods

Volumn 90, Issue 2, 2012, Pages 134-136

  Gabriel, Emma M ^a   Douarre, Pierre E ^a   Fitzgibbon, Siobhan ^b   Clair, James ^b   Lucey, Brigid ^a,c   Coffey, Aidan ^a   O'Mahony, Jim M ^a  

^a Munster Technological University   (Ireland)

^b MERCY UNIVERSITY HOSPITAL   (Ireland)

^c University College Cork and INFANT Centre   (Ireland)

Author keywords

G2576T mutation; High resolution melting; Linezolid resistance; Staphylococcus epidermidis

Indexed keywords

BACTERIAL RNA; GUANINE; LINEZOLID; RNA 23S; THYMINE;

ACCURACY; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; CONTROLLED STUDY; HIGH RESOLUTION MELTING ANALYSIS; INTERMETHOD COMPARISON; MINIMUM INHIBITORY CONCENTRATION; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION MAPPING; STAPHYLOCOCCUS EPIDERMIDIS;

ACETAMIDES; ANTI-BACTERIAL AGENTS; DNA, BACTERIAL; DNA, RIBOSOMAL; DRUG RESISTANCE, BACTERIAL; HUMANS; MICROBIAL SENSITIVITY TESTS; OXAZOLIDINONES; POINT MUTATION; RNA, BACTERIAL; RNA, RIBOSOMAL, 23S; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS EPIDERMIDIS; TRANSITION TEMPERATURE;

STAPHYLOCOCCUS EPIDERMIDIS;

EID: 84861738006     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.04.002     Document Type: Article

References (18)

1. Choi G.E., Lee S.M., Jongyoun Y., Hwang S.H., Kim H.H., Lee E.Y., et al. High-Resolution Melting Curve Analysis for Rapid Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Clinical Isolates. J. Clin. Microbiol. 2010, 48(11):3893-3898.
2. Clinical and Laboratory Standards Institute Performance standards for antimicrobial susceptibility testing, 21st informational supplement. CLSI document M100-S21 2011.
3. De Almeida L.M., Lincopan N., De Araujo M.R.E., Mamizukal E.M. Dissemination of the linezolid-resistant Staphylococcus epidermidis clone ST2 exhibiting the G2576T mutation in the 23S rRNA gene in a tertiary-care hospital, Brazil. J. Antimicrob. Chemother. 2012, 67(3):768-769.
4. Douarre P.E., Cashman W., Buckley J., Coffey A., O'Mahony J. High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis "cattle type" and "sheep type". J. Microbiol. Methods 2011, 88:172-174.
5. Erali Maria, Voelkerding K.V., Wittwer C.T. High Resolution Melting Applications for Clinical Laboratory Medicine. Exp. Mol. Pathol. 2008, 85(1):50-58.
6. Herschleb J., Ananiev G., Schwartz D.C. Pulsed-field gel electrophoresis. Nat. Protoc. 2007, 2:677-684.
7. Hong T., Li X., Wand J., Sloan C., Cicogna C. Sequential Linezolid-Resistant Staphylococcus epidermidis Isolates with G2576T Mutation. J. Clin. Microbiol. 2007, 45(10):3277-3280.
8. Long K.S., Vester B. Resistance to linezolid caused by modifications at its binding site on the ribosome. Antimicrob. Agents Chemother. 2012, 56(2):603-612.
9. Martineau F., Picard F.J., Roy P.H., Ouellette M., Bergeron M.G. Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus epidermidis. J. Clin. Microbiol. 1996, 34(12):2888-2893.
10. Meka V.G., Gold H.S. Antimicrobial Resistance to Linezolid. Clin. Infect. Dis. 2004, 39(7):1010-1015.
11. Mendes R.E., Deshpande L.M., Farrell D.J., Spanu T., Fadda G., Jones R.N. Assessment of linezolid resistance mechanisms among Staphylococcus epidermidis causing bacteraemia in Rome, Italy. J. Antimicrob. Chemother. 2010, 65:2329-2335.
12. Pillai S.K., Sakoulas G., Wennersten C., Eliopoulos G.M., Moellering R.C., Ferraro M.J., et al. Linezolid Resistance in Staphylococcus aureus: Characterisation and Stability of Resistant Phenotype. J. Infect. Dis. 2002, 186:1603-1607.
13. Reed G.H., Kent J.O., Wittwer C.T. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics 2007, 8:597-608.
14. Ruskova Lenka, Raclavsky Vladislav The potential of high resolution melting analysis (HRMA) to streamline, facilitate and enrich routine diagnostics in medical microbiology. Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech. Repub. 2011, 155(3):239-252.
15. Schommer N.N., Christner M., Hentschke M., Ruckdeschel K., Aepfelbacher M., Holger R. Staphylococcus epidermidis Uses Distinct Mechanisms of Biofilm Formation to Interfere with Phagocytosis and Activation of Mouse Macrophage-Like Cells 774A.1. Infect. Immun. 2011, 79(6):2267-2276.
16. Stefani S., Bongiorno D., Mongelli G., Campanile F. Linezolid Resistance in Staphylococci. Pharmaceuticals 2010, 3:1988-2006.
17. Stephens A.J., Inman-Bamber J., Giffard P.M., Huygens F. High-Resolution Melting Analysis of the spa Repeat Region of Staphylococcus aureus. Clin. Chem. 2008, 54:432-436.
18. Werner G., Bartel M., Wellington N., Essig A., Klare I., Wolfgang W., et al. Detection of Mutations Conferring Resistance to Linezolid in Enterococcus spp. by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 2007, 45(10):3421-3423.