High-resolution melting curve analysis for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical isolates

Journal of Clinical Microbiology

Volumn 48, Issue 11, 2010, Pages 3893-3898

  Choi, Go Eun ^a   Lee, Sun Min ^a   Yi, Jongyoun ^a   Hwang, Sang Hyun ^a   Kim, Hyung Hoi ^a   Lee, Eun Yup ^a   Cho, Eun Hae ^b   Kim, Jee Hee ^c   Kim, Hwa Jung ^d   Chang, Chulhun L ^a,e  

^a Pusan National University School of Medicine   (South Korea)

^b Green Cross Reference Laboratory   (South Korea)

^c Kangwon National University   (South Korea)

^d Chungnam National University   (South Korea)

^e Pusan National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ISONIAZID; KATG PROTEIN; RIFAMPICIN; RNA POLYMERASE BETA SUBUNIT;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; CODON; DNA FRAGMENTATION; DNA SEQUENCE; HIGH RESOLUTION MELTING CURVE ANALYSIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OUTCOME ASSESSMENT; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

ANTITUBERCULAR AGENTS; BACTERIAL PROTEINS; CATALASE; CODON; DNA PRIMERS; DNA, BACTERIAL; DNA-DIRECTED RNA POLYMERASES; DRUG RESISTANCE, BACTERIAL; HUMANS; ISONIAZID; MICROBIAL SENSITIVITY TESTS; MYCOBACTERIUM TUBERCULOSIS; OXIDOREDUCTASES; POLYMERASE CHAIN REACTION; RIFAMPIN; SENSITIVITY AND SPECIFICITY; TRANSITION TEMPERATURE; TUBERCULOSIS;

MYCOBACTERIUM TUBERCULOSIS;

EID: 78049522369     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00396-10     Document Type: Article

References (29)

1. Afanas'ev, M. V., L. N. Ikryannikova, E. N. Il'ina, S. V. Sidorenko, A. V. Kuz'min, E. E. Larionova, T. G. Smirnova, L. N. Chernousova, E. Y. Kamaev, S. N. Skorniakov, V. N. Kinsht, A. G. Cherednichenko, and V. M. Govorun. 2007. Molecular characteristics of rifampicin- and isoniazid-resistant Mycobacterium tuberculosis isolates from the Russian Federation. J. Antimicrob. Chemother. 59:1057-1064.
2. Ahmad, S., and E. Mokaddas. 2009. Recent advances in the diagnosis and treatment of multidrug-resistant tuberculosis. Respir. Med. 103:1777-1790.
3. Bai, G. H., S. J. Kim, and C. L. Chang. 2007. Proficiency analysis of drug susceptibility testing by national-level tuberculosis reference laboratories from 1995 to 2003. J. Clin. Microbiol. 45:3626-3630.
4. Bartfai, Z., A. Somoskovi, C. Kodmon, N. Szabo, E. Puskas, L. Kosztolanyi, E. Farago, J. Mester, L. M. Parsons, and M. Salfinger. 2001. Molecular characterization of rifampin-resistant isolates of Mycobacterium tuberculosis from Hungary by DNA sequencing and the line probe assay. J. Clin. Microbiol. 39:3736-3739.
5. Caws, M., and F. A. Drobniewski. 2001. Molecular techniques in the diagnosis of Mycobacterium tuberculosis and the detection of drug resistance. Ann. N. Y. Acad. Sci. 953:138-145.
6. Centers for Disease Control. 1990. Nosocomial transmission of multidrug-resistant tuberculosis to health-care workers and HIV-infected patients in an urban hospital - Florida. MMWR Morb. Mortal. Wkly. Rep. 39:718-722.
7. Centers for Disease Control and Prevention. 2006. Emergence of Mycobacterium tuberculosis with extensive resistance to second-line drugs - worldwide, 2000-2004. MMWR Morb. Mortal. Wkly. Rep. 55:301-305.
8. Centers for Disease Control and Prevention and American Thoracic Society. 2003. Update: adverse event data and revised American Thoracic Society/CDC recommendations against the use of rifampin and pyrazinamide for treatment of latent tuberculosis infection - United States, 2003. MMWR Morb. Mortal. Wkly. Rep. 52:735-739.
9. Cho, E. H., H. K. Bae, S. K. Kang, and E. H. Lee. 2009. Detection of isoniazid and rifampicin resistance by sequencing of katG, inhA, and rpoB genes in Korea. Korean J. Lab. Med. 29:455-460.
10. Cirillo, D. M., F. Piana, L. Frisicale, M. Quaranta, A. Riccabone, V. Penati, P. Vaccarino, and G. Marchiaro. 2004. Direct rapid diagnosis of rifampicinresistant M. tuberculosis infection in clinical samples by line probe assay (INNO LiPA Rif-TB). New Microbiol. 27:221-227.
11. Garcia de Viedma, D. 2003. Rapid detection of resistance in Mycobacterium tuberculosis: a review discussing molecular approaches. Clin. Microbiol. Infect. 9:349-359.
12. Heep, M., U. Rieger, D. Beck, and N. Lehn. 2000. Mutations in the beginning of the rpoB gene can induce resistance to rifamycins in both Helicobacter pylori and Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 44:1075-1077. (Pubitemid 30165295)
13. Herrmann, M. G., J. D. Durtschi, L. K. Bromley, C. T. Wittwer, and K. V. Voelkerding. 2006. Amplicon DNA melting analysis for mutation scanning and genotyping: cross-platform comparison of instruments and dyes. Clin. Chem. 52:494-503.
14. Hoek, K. G., N. C. Gey van Pittius, H. Moolman-Smook, K. Carelse-Tofa, A. Jordaan, G. D. van der Spuy, E. Streicher, T. C. Victor, P. D. van Helden, and R. M. Warren. 2008. Fluorometric assay for testing rifampin susceptibility of Mycobacterium tuberculosis complex. J. Clin. Microbiol. 46:1369-1373.
15. Huang, W. L., H. Y. Chen, Y. M. Kuo, and R. Jou. 2009. Performance assessment of the GenoType MTBDRplus test and DNA sequencing in detection of multidrug-resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 47:2520-2524.
16. Kim, S. Y., Y. J. Park, W. I. Kim, S. H. Lee, C. L. Chang, S. J. Kang, and C. S. Kang. 2003. Molecular analysis of isoniazid resistance in Mycobacterium tuberculosis isolates recovered from South Korea. Diagn. Microbiol. Infect. Dis. 47:497-502.
17. Kocagoz, T., Z. Saribas, and A. Alp. 2005. Rapid determination of rifampin resistance in clinical isolates of Mycobacterium tuberculosis by real-time PCR. J. Clin. Microbiol. 43:6015-6019.
18. Makinen, J., H. J. Marttila, M. Marjamaki, M. K. Viljanen, and H. Soini. 2006. Comparison of two commercially available DNA line probe assays for detection of multidrug-resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 44:350-352.
19. Montgomery, J., C. T. Wittwer, R. Palais, and L. Zhou. 2007. Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis. Nat. Protoc. 2:59-66.
20. Ong, D. C., W. C. Yam, G. K. Siu, and A. S. Lee. 2010. Rapid detection of rifampin- and isoniazid-resistant Mycobacterium tuberculosis by high-resolution melting analysis. J. Clin. Microbiol. 48:1047-1054.
21. Park, H., E. J. Song, E. S. Song, E. Y. Lee, C. M. Kim, S. H. Jeong, J. H. Shin, J. Jeong, S. Kim, Y. K. Park, G. H. Bai, and C. L. Chang. 2006. Comparison of a conventional antimicrobial susceptibility assay to an oligonucleotide chip system for detection of drug resistance in Mycobacterium tuberculosis isolates. J. Clin. Microbiol. 44:1619-1624.
22. Park, Y. K., S. Shin, S. Ryu, S. N. Cho, W. J. Koh, O. J. Kwon, Y. S. Shim, W. J. Lew, and G. H. Bai. 2005. Comparison of drug resistance genotypes between Beijing and non-Beijing family strains of Mycobacterium tuberculosis in Korea. J. Microbiol. Methods 63:165-172.
23. Pietzka, A. T., A. Indra, A. Stoger, J. Zeinzinger, M. Konrad, P. Hasenberger, F. Allerberger, and W. Ruppitsch. 2009. Rapid identification of multidrug-resistant Mycobacterium tuberculosis isolates by rpoB gene scanning using high-resolution melting curve PCR analysis. J. Antimicrob. Chemother. 63:1121-1127.
24. Ramaswamy, S. V., R. Reich, S. J. Dou, L. Jasperse, X. Pan, A. Wanger, T. Quitugua, and E. A. Graviss. 2003. Single nucleotide polymorphisms in genes associated with isoniazid resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 47:1241-1250.
25. Sekiguchi, J., T. Miyoshi-Akiyama, E. Augustynowicz-Kopec, Z. Zwolska, F. Kirikae, E. Toyota, I. Kobayashi, K. Morita, K. Kudo, S. Kato, T. Kuratsuji, T. Mori, and T. Kirikae. 2007. Detection of multidrug resistance in Mycobacterium tuberculosis. J. Clin. Microbiol. 45:179-192.
26. van Soolingen, D., P. W. Hermans, P. E. de Haas, D. R. Soll, and J. D. van Embden. 1991. Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J. Clin. Microbiol. 29:2578-2586.
27. Wada, T., S. Maeda, A. Tamaru, S. Imai, A. Hase, and K. Kobayashi. 2004. Dual-probe assay for rapid detection of drug-resistant Mycobacterium tuberculosis by real-time PCR. J. Clin. Microbiol. 42:5277-5285.
28. Watterson, S. A., S. M. Wilson, M. D. Yates, and F. A. Drobniewski. 1998. Comparison of three molecular assays for rapid detection of rifampin resistance in Mycobacterium tuberculosis. J. Clin. Microbiol. 36:1969-1973.
29. Wittwer, C. T., G. H. Reed, C. N. Gundry, J. G. Vandersteen, and R. J. Pryor. 2003. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin. Chem. 49:853-860.