High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep type"

Journal of Microbiological Methods

Volumn 88, Issue 1, 2012, Pages 172-174

  Douarre, Pierre E ^a   Cashman, William ^b   Buckley, Jim ^b   Coffey, Aidan ^a   O'Mahony, Jim M ^a  

^a Munster Technological University   (Ireland)

^b Forward Planning and Strategic Development   (Ireland)

Author keywords

Genotyping; High resolution melting; Mycobacterium avium subsp. paratuberculosis; PCR

Indexed keywords

ARTICLE; BACTERIUM IDENTIFICATION; CATTLE; CONTROLLED STUDY; GENETIC POLYMORPHISM; HIGH RESOLUTION MELTING ANALYSIS; MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM HOMINISSUIS; MYCOBACTERIUM INTRACELLULARE AVIUM; MYCOBACTERIUM PARATUBERCULOSIS; MYCOBACTERIUM SILVATICUM; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SHEEP; SPECIES DIFFERENTIATION; TANDEM REPEAT;

ANIMALS; BACTERIAL TYPING TECHNIQUES; CATTLE; CATTLE DISEASES; GENOTYPE; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; PARATUBERCULOSIS; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; SHEEP; SHEEP DISEASES; TANDEM REPEAT SEQUENCES;

BOS; MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS; OVIS ARIES;

EID: 83955165363     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.10.009     Document Type: Article

References (13)

1. Alvarez J., Garcia I.G., Aranaz A., Bezos J., Romero B., de Juan L., et al. Genetic diversity of Mycobacterium avium isolates recovered from clinical samples and from the environment: molecular characterization for diagnostic purposes. J. Clin. Microbiol. 2008, 46:1246-1251.
2. Collins D.M., Gabric D.M., de Lisle G.W. Identification of two groups of Mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization. J. Clin. Microbiol. 1990, 28:1591-1596.
3. Collins D.M., De Zoete M., Cavaignac S.M. Mycobacterium avium subsp paratuberculosis strains from cattle and sheep can be distinguished by a PCR test based on a novel DNA sequence difference. J. Clin. Microbiol. 2002, 40:4760-4762.
4. Douarre P.E., Cashman W., Buckley J., Coffey A., O'Mahony J. Molecular characterization of Mycobacterium avium subsp. paratuberculosis using multi-locus short sequence repeat (MLSSR) and mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) typing methods. Vet. Microbiol. 2011, 149:3-4.
5. Motiwala A.S., Li L., Kapur V., Sreevatsan S. Current understanding of the genetic diversity of Mycobacterium avium subsp. paratuberculosis. Microbes Infect. 2006, 8:1406-1418.
6. O'Mahony J., Hill C. Rapid real-time PCR assay for detection and quantitation of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk. Appl. Environ. Microbiol. 2004, 2070:4561-4568.
7. Odell I.D., Cloud J.L., Seipp M., Wittwer C.T. Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting analysis of hsp65 PCR products. Am. J. Clin. Pathol. 2005, 123:96-101.
8. Pang Y., Zhou Y., Wang S., Lu J., Lu B., He G., Wang L., Zhao Y. A novel method based on high resolution melting (HRM) analysis for MIRU-VNTR genotyping of Mycobacterium tuberculosis. J. Microbiol. Methods 2011, 86:291-297.
9. Reed G.H., Kent J.O., Wittwer C.T. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics 2007, 8:597-608.
10. Semret M., Turenne C.Y., de Haas P., Collins D.M., Behr M.A. Differentiating host-associated variants of Mycobacterium avium by PCR for detection of large sequence polymorphisms. J. Clin. Microbiol. 2006, 44:881-887.
11. Sohal J.S., Singh S.V., Singh A.V., Singh P.K. Strain diversity within Mycobacterium avium subspecies paratuberculosis-a review. Indian J. Exp. Biol. 2010, 48:7-16.
12. Stevenson K., Hughes V.M., de Juan L., Inglis N.F., Wright F., Sharp J.M., et al. Molecular characterization of pigmented and nonpigmented isolates of Mycobacterium avium subsp. paratuberculosis. J. Clin. Microbiol. 2002, 40:1798-1804.
13. Stevenson K., Alvarez J., Bakker D., Biet F., de Juan L., Denham S., et al. Occurrence of Mycobacterium avium subspecies paratuberculosis across host species and European countries with evidence for transmission between wildlife and domestic ruminants. BMC Microbiol. 2009, 9:212.