Combined use of laser capture microdissection and cDNA microarray analysis identifies locally expressed disease-related genes in focal regions of psoriasis vulgaris skin lesions

Journal of Investigative Dermatology

Volumn 132, Issue 6, 2012, Pages 1615-1626

  Mitsui, Hiroshi ^a   Suárez Fariñas, Mayte ^a,b   Belkin, Daniel A ^a   Levenkova, Natasha ^a   Fuentes Duculan, Judilyn ^a   Coats, Israel ^a   Fujita, Hideki ^a   Krueger, James G ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD83 ANTIGEN; CHEMOKINE RECEPTOR CCR7; COMPLEMENTARY DNA; DENDRITIC CELL LYSOSOME ASSOCIATED MEMBRANE PROTEIN; MACROPHAGE INFLAMMATORY PROTEIN 3BETA;

ARTICLE; CELL MATURATION; CONTROLLED STUDY; CYTOKINE PRODUCTION; DENDRITIC CELL; DERMIS; EPIDERMIS; GENE EXPRESSION; GENE IDENTIFICATION; HUMAN; HUMAN CELL; LASER CAPTURE MICRODISSECTION; LYMPHOID CELL; MICROARRAY ANALYSIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; PSORIASIS VULGARIS;

EID: 84861202033     PISSN: 0022202X     EISSN: 15231747     Source Type: Journal    
DOI: 10.1038/jid.2012.33     Document Type: Article

References (35)

1. Bowcock AM, Shannon W, Du F et al. (2001) Insights into psoriasis and other inflammatory diseases from large-scale gene expression studies. Hum Mol Genet 10:1793-805 (Pubitemid 32899097)
2. Braun S, Hanselmann C, Gassmann MG et al. (2002) Nrf2 transcription factor, a novel target of keratinocyte growth factor action which regulates gene expression and inflammation in the healing skin wound. Mol Cell Biol 22:5492-505 (Pubitemid 34755759)
3. Clark RA (2010) Skin-resident T cells: the ups and downs of on site immunity. J Invest Dematol 130:362-70
4. Clark RA, Chong B, Mirchandani N et al. (2006) The vast majority of CLA+ T cells are resident in normal skin. J Immunol 176:4431-9 (Pubitemid 44264423)
5. Clément-Ziza M, Gentien D, Lyonnet S et al. (2009) Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling. BMC Genomics 10:246-60
6. Clemmesen A, Thomassen M, Clemmesen O et al. (2009) Extraction of highquality epidermal RNA after ammonium thiocyanate-induced dermoepidermal separation of 4mm human skin biopsies. Exp Dermatol 18:979-84
7. de Bruin EC, van de Pas S, Lips EH et al. (2005) Macrodissection versus microdissection of rectal carcinoma: minor influence of stroma cells to tumor cell gene expression profiles. BMC Genomics 14:142
8. de Guzman Strong C, Wertz PW, Wang C et al. (2006) Lipid defect underlies selective skin barrier impairment of an epidermal-specific deletion of Gata-3. J Cell Biol 175:661-70 (Pubitemid 44790618)
9. Espina V, Wulfkuhle JD, Calvert VS et al. (2006) Laser-capture microdissection. Nat Protoc 1:586-603
10. Ganguly D, Chamilos G, Lande R et al. (2009) Self-RNA-antimicrobial peptide complexes activate human dendritic cells through TLR7 and TLR8. J Exp Med 206:1983-94
11. Gudjonsson JE, Ding J, Johnston A et al. (2010) Assessment of the psoriatic transcriptome in a large sample: additional regulated genes and comparisons with in vitro models. J Invest Dermatol 130:1829-40
12. Haider AS, Lowes MA, Suárez-Farinãs M et al. (2008) Cellular genomic maps help dissect pathology in human skin disease. J Invest Dermatol 128:606-15 (Pubitemid 351252667)
13. Ho IC, Tai TS, Pai SY (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol 9:125-35
14. Jabbari A, Suárez-Farinãs M, Dewell S et al. (2012) Transcriptional profiling of psoriasis using RNA-seq reveals previously unidentified differentially expressed genes. J Invest Dermatol 132:246-9
15. Kennedy-Crispin M, Billick E, Mitsui H et al. (2012) Human keratinocytes have a response to injury that upregulates CCL20 and other genes linking innate and adaptive immunity. J Invest Dermatol 132:105-13
16. Klee EW, Erdogan S, Tillmans L et al. (2009) Impact of sample acquisition and linear amplification on gene expression profiling of lung adenocarcinoma: laser capture micro-dissection cell-sampling versus bulk tissuesampling. BMC Med Genomics 2:13-23
17. Kobayashi A, Ito E, Toki T et al. (1999) Molecular cloning and functional characterization of a new cap'n' collar family transcription factor Nrf3. J Biol Chem 274:6443-52 (Pubitemid 29111059)
18. Kube DM, Savci-Heijink CD, Lamblin AF et al. (2007) Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer. BMC Mol Biol 8:25-38
19. Lowes MA, Chamian F, Abello MV et al. (2005) Increase in TNF-alpha and inducible nitric oxide synthase-expressing dendritic cells in psoriasis and reduction with efalizumab (anti-CD11a). Proc Natl Acad Sci USA 102:19057-62 (Pubitemid 43049565)
20. Luther SA, Bidgol A, Hargreaves DC et al. (2002) Differing activities of homeostatic chemokines CCL19, CCL21, and CXCL12 in lymphocyte and dendritic cell recruitment and lymphoid neogenesis. J Immunol 169:424-33 (Pubitemid 34686164)
21. Luzzi V, Mahadevappa M, Raja R et al. (2003) Accurate and reproducible gene expression profiles from laser capture microdissection, transcript amplification, and high density oligonucleotide microarray analysis. J Mol Diagn 5:9-14 (Pubitemid 36192994)
22. Mansbridge JN, Knapp AM (1987) Changes in keratinocyte maturation during wound healing. J Invest Dermatol 89:253-63 (Pubitemid 17135369)
23. Mizutani Y, Kihara A, Igarashi Y (2005) Mammalian Lass6 and its related family members regulate synthesis of specific ceramides. Biochem J 390:263-71 (Pubitemid 41192252)
24. Motohashi H, Katsuoka F, Engel JD et al. (2004) Small Maf proteins serve as transcriptional cofactors for keratinocyte differentiation in the Keap1- Nrf2 regulatory pathway. Proc Natl Acad Sci USA 101:6379-84 (Pubitemid 38585982)
25. Singh R, Maganti RJ, Jabba SV et al. (2005) Microarray-based comparison of three amplification methods for nanogram amounts of total RNA. Am J Physiol Cell Physiol 288:1179-89
26. Suárez-Farinãs M, Lowes MA, Zaba LC et al. (2010) Evaluation of he psoriasis transcriptome across different studies by gene set enrichment analysis (GSEA). PLOS One 5:e10247
27. Suárez-Farinãs M, Pellegrino M, Wittkowski KM et al. (2005) Harshlight: a "corrective make-up" program for microarray chips. BMC Bioinformatics 6:294-304
28. Takahashi H, Oyama N, Itoh Y et al. (2000) Transcriptional factor AP- 2gamma increases human cystatin A gene transcription of keratinocytes. Biochem Biophys Res Commun 278:719-23
29. Wang E, Panelli M, Marincola FM (2007) Complementary techniques: RNA amplification for gene profiling analysis. Adv Exp Med Biol 593:39-53
30. Wingens M, van Bergen BH, Hiemstra PS et al. (1998) Induction of SLPI (ALP/HUSI-I) in epidermal keratinocytes. J Invest Dermatol 111:996-1002 (Pubitemid 29115165)
31. Yao Y, Richman L, Morehouse C et al. (2008) Type I interferon: potential therapeutic target for psoriasis? PLoS One 3:e2737
32. Zaba LC, Cardinale I, Gilleaudeau P et al. (2007) Amelioration of epidermal hyperplasia by TNF inhibition is associated with reduced Th17 responses. J Exp Med 204:3183-94
33. Zaba LC, Fuentes-Duculan J, Eungdamrong NJ et al. (2009) Psoriasis is characterized by accumulation of immunostimulatory and Th1/Th17 cell-polarizing myeloid dendritic cells. J Invest Dermatol 129:79-88
34. Zaba LC, Fuentes-Duculan J, Eungdamrong NJ et al. (2010) Identification of TNF-related apoptosis-inducing ligand and other molecules that distinguish inflammatory from resident dendritic cells in patients with psoriasis. J Allergy Clin Immunol 125:1261-8
35. Zhou X, Krueger JG, Kao M-C et al. (2003) Novel mechanisms of T-cell and dendritic cell activation revealed by profiling of psoriasis on the 63,100- element oligonucleotide array. Physiol Genomics 13:59-78