Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp.

Journal of Clinical Microbiology

Volumn 42, Issue 12, 2004, Pages 5849-5853

  Aranda, K R S ^a   Fagundes Neto, U ^a   Scaletsky, I C A ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

SHIGA TOXIN;

AMPLICON; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; DIARRHEA; DNA HYBRIDIZATION; DNA PROBE; ENTEROBACTER INFECTION; ESCHERICHIA COLI; FECES ANALYSIS; GENE TARGETING; HUMAN; INFANT; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SHIGELLA; SHIGELLOSIS;

CHILD, PRESCHOOL; DIARRHEA; DYSENTERY, BACILLARY; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FECES; HUMANS; INFANT; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN; SHIGELLA;

BACTERIA (MICROORGANISMS); ENTEROBACTER; ESCHERICHIA COLI; NEGIBACTERIA; SHIGELLA;

EID: 10844230042     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.42.12.5849-5853.2004     Document Type: Article

References (18)

1. Baudry, B., S. J. Savarino, P. Vial, J. B. Kaper, and M. M. Levine. 1990. A sensitive and specific DNA probe to identify enteroaggregative E. coli, a recently discovered diarrheal pathogen. J. Infect. Dis. 161:1249-1251.
2. Benz, I., and M. A. Schmidt. 1989. Cloning and expression of an adhesin (AIDA-I) involved in diffuse adherence of enteropathogenic Escherichia coli. Infect. Immun. 57:1506-1511.
3. Bilge, S. S., C. R. Clausen, W. Lau, and S. L. Moseley. 1989. Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. J. Bacteriol. 171:4281-4289.
4. Dulguer, M. V., S. H. Fabbricotti, S. Y. Bando, C. A. Moreira-Filho, U. Fagundes-Neto, and I. C. A. Scaletsky. 2003. Atypical enteropathogenic Escherichia coli strains: phenotypic and genetic profiling reveals a strong association between enteroaggregative E. coli heat-stable enterotoxin and diarrhea. J. Infect. Dis. 188:1685-1694.
5. Gunzburg, S. T., N. G. Tornieporth, and L. W. Riley. 1995. Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene. J. Clin. Microbiol. 33:1375-1377.
6. Jerse, A. E., Y. Jun, B. D. Tall, and J. B. Kaper. 1990. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc. Natl. Acad. Sci. USA 87:7839-7843.
7. Moseley, S. L., I. Huq, A. R. M. A. Alim, M. So, M. Samadpour-Motalebi, and S. Falkow. 1980. Detection of enterotoxigenic Escherichia coli by DNA colony hybridization. J. Infect. Dis. 142:892-898.
8. Nataro, J. P., M. M. Baldini, J. B. Kaper, R. E. Black, N. Bravo, and M. M. Levine. 1985. Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe. J. Infect. Dis. 152:560-565.
9. Nataro, J. P., and J. B. Kaper. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142-201.
10. Newland, J. W., and R. J. Neill. 1988. DNA probes for Shiga-like toxins I and II and for toxin-converting bacteriophages. J. Clin. Microbiol. 26:1292-1297.
11. O157. J. Clin. Microbiol. 36:598-602.
12. Reid, S. D., D. J. Betting, and T. S. Whittam. 1999. Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR. J. Clin. Microbiol. 37:2719-2722.
13. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
14. Scaletsky, I. C. A., S. H. Fabbricotti, K. R. Aranda, M. B. Morais, and U. Fagundes-Neto. 2002. Comparison of DNA hybridization and PCR assays for detection of putative pathogenic enteroadherent Escherichia coli. J. Clin. Microbiol. 40:1254-1258.
15. Schmidt, H., C. Knop, S. Franke, S. Aleksic, J. Heesemann, and H. Karch. 1995. Development of PCR for screening of enteroaggregative Escherichia coli. J. Clin. Microbiol. 33:701-705.
16. Sethabutr, O., M. Venkatesan, G. S. Murphy, B. Eampokalap, C. W. Hoge, and P. Echeverria. 1993. Detection of Shigella and enteroinvasive Escherichia coli by amplification of the invasion plasmid antigen H DNA sequence in patients with dysentery. J. Infect. Dis. 167:458-461.
17. Small, P. L., and S. Falkow. 1986. Development of a DNA probe for the virulence plasmid of Shigella spp. and enteroinvasive Escherichia coli, p. 121-124. In L. Leive, P. F. Bonventre, J. A. Morello, S. D. Silver, and W. C. Wu (ed.), Microbiology - 1986. American Society for Microbiology, Washington, D.C.
18. Stacy-Phipps, S., J. J. Mecca, and J. B. Weiss. 1995. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection. J. Clin. Microbiol. 33:1054-1059.