Versatile transformation system that is applicable to both multiple transgene expression and gene targeting for thraustochytrids

Applied and Environmental Microbiology

Volumn 78, Issue 9, 2012, Pages 3193-3202

  Sakaguchi, Keishi ^a   Matsuda, Takanori ^a   Kobayashi, Takumi ^a   Ohara, Jun Ichiro ^a   Hamaguchi, Rie ^a   Abe, Eriko ^a   Nagano, Naoki ^b   Hayashi, Masahiro ^b   Ueda, Mayumi ^c   Honda, Daiske ^c   Okita, Yuji ^d   Taoka, Yousuke ^d   Sugimoto, Shinichi ^d   Okino, Nozomu ^a   Ito, Makoto ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b UNIVERSITY OF MIYAZAKI   (Japan)

^c KONAN UNIVERSITY   (Japan)

^d NIPPON SUISAN KAISHA LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ARACHIDONIC ACIDS; AURANTIOCHYTRIUM; DESATURASE; EICOSAPENTAENOIC ACID; ENHANCED GREEN FLUORESCENT PROTEIN; GENE TARGETING; HOMOLOGOUS RECOMBINATION; HYGROMYCIN; LINOLENIC ACIDS; NEOMYCIN RESISTANCE; SCHIZOCHYTRIUM; SELECTABLE MARKER GENES; THRAUSTOCHYTRIDS; THRAUSTOCHYTRIUM; TRANSFORMATION SYSTEMS; TRANSGENE EXPRESSION; UBIQUITIN;

GENES; GENETIC ENGINEERING; NUCLEIC ACIDS; POLYUNSATURATED FATTY ACIDS; PROTEINS;

FATTY ACIDS;

ACYL COENZYME A DESATURASE; ANTIINFECTIVE AGENT; GREEN FLUORESCENT PROTEIN; RNA 18S;

CHROMOSOME; ENZYME ACTIVITY; GENE EXPRESSION; GROWTH RATE; PROTEIN; PROTIST; RECOMBINATION;

ARTICLE; GENE DELETION; GENE EXPRESSION; GENE TARGETING; GENE TRANSFER; GENETIC RECOMBINATION; GENETIC SELECTION; GENETIC TRANSFORMATION; GENETICS; METABOLISM; METHODOLOGY; MICROBIAL GENETICS; REPORTER GENE; STRAMENOPILE;

ANTI-INFECTIVE AGENTS; FATTY ACID DESATURASES; GENE DELETION; GENE EXPRESSION; GENE TARGETING; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GENETICS, MICROBIAL; GREEN FLUORESCENT PROTEINS; RECOMBINATION, GENETIC; RNA, RIBOSOMAL, 18S; SELECTION, GENETIC; STRAMENOPILES; TRANSFORMATION, GENETIC;

SCHIZOCHYTRIUM; THRAUSTOCHYTRIIDAE; THRAUSTOCHYTRIUM; THRAUSTOCHYTRIUM AUREUM;

EID: 84861170783     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.07129-11     Document Type: Article

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