Melting curve analysis using hybridisation probes: Limitations in microbial molecular diagnostics [1]

Pathology

Volumn 37, Issue 3, 2005, Pages 254-256

  Whiley, David M ^a,b   Sloots, Theo P ^a,b,c  

^a Royal Children's Hospital and Health Service District   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c ROYAL BRISBANE AND WOMEN'S HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; GLYCOPROTEIN D; OLIGONUCLEOTIDE;

BK VIRUS; DIAGNOSTIC PROCEDURE; GENE; HERPES SIMPLEX VIRUS; HYBRIDIZATION; JC VIRUS; LETTER; MELTING POINT; MICROORGANISM; POLYMERASE CHAIN REACTION; POLYOMA VIRUS;

EID: 21744457301     PISSN: 00313025     EISSN: 14653931     Source Type: Journal    
DOI: 10.1080/00313020500099270     Document Type: Article

References (7)

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2. Whiley DM, Mackay IM, Syrmis MW, Win MJ, Sloots TP. Detection and differentiation of herpes simplex virus types 1 and 2 by a duplex LightCycler PCR that incorporates an internal control PCR reaction. J Clin Virol 2004; 30: 32-8.
3. Burrows J, Nitsche A, Bayly B, Walker E, Higgins G, Kok T. Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture. BMC Microbiol 2002; 9: 12.
4. Schalasta G, Arents A, Schmid M, Braun RW, Enders G. Fast and type-specific analysis of herpes simplex virus types 1 and 2 by rapid PCR and fluorescence melting-curve-analysis. Infection 2000; 28: 85-91.
5. Anderson TP, Werno AM, Beynon KA, Murdoch DR. Failure to genotype herpes simplex virus by real-time PCR assay and melting curve analysis due to sequence variation within probe binding sites. J Clin Microbiol 2003; 41: 2135-7.
6. Whiley DM, Syrmis MW, Mackay IM, Sloots TP. Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens. Eur J Clin Microbiol Infect Dis 2003; 22: 764-7.
7. Whiley DM, Mackay IM, Sloots TP. Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR. J Clin Microbiol 2001; 39: 4357-61.