Suction sampling as a significant source of error in molecular analysis of predator diets

Bulletin of Entomological Research

Volumn 102, Issue 3, 2012, Pages 261-266

  King, R A ^a,c   Davey, J S ^a   Bell, J R ^a,b   Read, D S ^a,d   Bohan, D A ^b,e   Symondson, W O C ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b ROTHAMSTED RESEARCH   (United Kingdom)

^c UNIVERSITY OF EXETER   (United Kingdom)

^d CENTRE FOR ECOLOGY AND HYDROLOGY   (United Kingdom)

^e CEMAGREF   (France)

Author keywords

aphid; carabid; Collembola; false positive; spider

Indexed keywords

ERROR ANALYSIS; FECES; INSECT; MOLECULAR ANALYSIS; POLLUTANT SOURCE; POLLUTION EFFECT; PREDATION; PREDATOR-PREY INTERACTION; SAMPLING;

ANIMAL; ARTICLE; BEETLE; DIET; ECOLOGY; FOOD CHAIN; LABORATORY DIAGNOSIS; METHODOLOGY; POLYMERASE CHAIN REACTION; PREDATION; SPIDER; STARVATION;

ANIMALS; BEETLES; DIET; ECOLOGY; FALSE POSITIVE REACTIONS; FOOD CHAIN; POLYMERASE CHAIN REACTION; PREDATORY BEHAVIOR; SPIDERS; STARVATION;

APHIDIDAE; ARANEAE; CARABIDAE; COLLEMBOLA;

EID: 84861092277     PISSN: 00074853     EISSN: 14752670     Source Type: Journal    
DOI: 10.1017/S0007485311000575     Document Type: Article

References (29)

1. Bell, J.R., Wheater, C.P., Henderson, R. & Cullen, W.R. (2002) Testing the efficiency of suction samplers (G-vacs) on spiders. The effects of increasing nozzle size and suction time. pp. 285-290 in Toft, S. & Scharff, N. (Eds) European Arachnology 2000. Århus, Denmark, Aarhus University Press.
2. Bell, J.R., King, R.A., Bohan, D.A. & Symondson,W.O.C. (2010) Spatial co-occurrence networks coupled with molecular analysis of trophic links reveal the spatial dynamics and feeding histories of polyphagous predators. Ecography 33, 64-72.
3. Chapman, E.G., Romero, S.A. & Harwood, J.D. (2010) Maximizing collection and minimizing risk: does vacuum suction sampling increase the likelihood for misinterpretation of food web connections? Molecular Ecology Resources 10, 1023-1033.
4. Chen, Y., Giles, K.L., Payton, M.E. & Greenstone, M.H. (2000) Identifying key cereal aphid predators by molecular gut analysis. Molecular Ecology 9, 1887-1898.
5. Davey, J.S. (2010) Intraguild predation among generalist predators in winter wheat. PhD thesis, University of Cardiff, Cardiff, UK.
6. Folmer, O., Black, M., Hoeh,W., Lutz, R.& Vrijenhoek, R. (1994) DNA primers for the amplification of mitochondrialcytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294-299.
7. Foltan, P., Sheppard, S., Konvicka, M.Symondson, W.O.C. (2005) The significance of facultative scavenging in generalist predator nutrition: detecting decayed prey in the guts of predators using PCR. Molecular Ecology 14, 4147-4158. (Pubitemid 44943744)
8. Greenstone, M.H., Weber, D.C., Coudron, T.C. & Payton, M.E. (2011) Unnecessary roughness? Testing the hypothesis that predators destined for molecular gut-content analysis must be hand-collected to avoid cross-contamination. Molecular Ecology Resources 11, 286-293.
9. Harwood, J.D. (2008) Are sweep net sampling and pitfall trapping compatible with molecular analysis of predation? Environmental Entomology 37, 990-995.
10. Hebert, L., Darden, S.K., Pedersen, B.V. & Dabelsteen, T. (2011) IncreasedDNAamplification success of non-invasive genetic samples by successful removal of inhibitors from faecal samples collected in the field. Conservation Genetics Resources 3, 41-43.
11. Hoogendoorn, M.& Heimpel, G.E. 2001) PCR-based gut content analysis of insect predators: using ribosomal ITS-1 fragments from prey to estimate predation frequency. Molecular Ecology 10 2059-2067. (Pubitemid 32792460)
12. Juen, A. & Traugott, M. (2005) Detecting predation and scavenging by DNA gut-content analysis: a case study using a soil insect predator-prey system. Oecologia 142, 344-352. (Pubitemid 40171963)
13. Juen, A. & Traugott, M. (2006) Amplification facilitators and multiplex PCR: tools to overcome PCR-inhibition in DNAgut- content analysis of soil-living invertebrates. Soil Biology and Biochemistry 38, 1872-1879. (Pubitemid 44067131)
14. Juen, A. & Traugott, M. (2007) Revealing species-specific trophic links in soil food webs: molecular identification of scarab predators. Molecular Ecology 16, 1545-1557. (Pubitemid 46474769)
15. King, R.A., Read, D.S., Traugott, M. & Symondson, W.O.C. (2008) Molecular analysis of predation: a review of best practice for DNA-based approaches. Molecular Ecology 17, 947-963. (Pubitemid 351197358)
16. King, R.A., Moreno-Ripoll, R., Agustí, N., Shayler, S.P., Bell, J. R., Bohan, D.A. & Symondson, W.O.C. (2011) Multiplex reactions for the molecular detection of predation on pest and non-pest invertebrates in agroecosystems. Molecular Ecology Resources 11, 370-373.
17. Kruse, P.D., Toft, S. & Sunderland, K.D. (2008) Temperature and prey capture: opposite relationships in two predator taxa. Ecological Entomology 33, 305-312. (Pubitemid 351341435)
18. Kuusk, A.K. & Agustí, N. (2008) Group-specific primers for DNA-based detection of springtails (Hexapoda: Collembola) within predator gut contents. Molecular Ecology Resources 8, 678-681. (Pubitemid 351703614)
19. Minitab Inc. (2008) Minitab Statistical Software, Release 15. Available online at http://www.minitab.com (accessed September 2011).
20. Pons, J. (2006) DNA-based identification of preys from nondestructive, total DNA extractions of predators using arthropod universal primers. Molecular Ecology Notes 6, 623-626. (Pubitemid 44252558)
21. Read, D.S. (2007) Molecular analysis of subterranean detritivore food webs. PhD thesis, University of Cardiff, Cardiff, UK.
22. Remén, C., Krüger, M.&Cassel-Lundhagen, A. (2010) Successful analysis of gut contents in fungal-feeding oribatid mites by combining body-surface washing and PCR. Soil Biology and Biochemistry 42, 1952-1957.
23. Sheppard, S.K., Bell, J., Sunderland, K.D., Fenlon, J., Skervin, D. & Symondson, W.O.C. (2005) Detection of secondary predation by PCR analyses of the gut contents of invertebrate generalist predators. Molecular Ecology 14, 4461-4468. (Pubitemid 44943723)
24. Sunderland, K.D., Powell, W., Symondson, W.O.C. (2005) Populations and communities. pp. 299-434 in Jervis, M.A. (Ed.) Insects as Natural Enemies: A Practical Perspective. Berlin, Germany, Springer.
25. Symondson, W.O.C. (2002) Molecular identification of prey in predator diets. Molecular Ecology 11, 627-641. (Pubitemid 34443719)
26. Virant-Doberlet, M., King, R.A., Polajnar, J. & Symondson, W. O.C. (2011) Molecular diagnostics reveal spiders that exploit prey vibrational signals used in sexual communication. Molecular Ecology 20, 2204-2216.
27. von (Pubitemid 351693045)
28. Wheater, C.P., Bell, J.R.&Cook, P.A. (2011) Practical Field Ecology: A Project Guide. London, UK, Wiley-Blackwell.
29. Wootton, J.T.&Emmerson, M. 2005) Measurement of interaction strength in nature. Annual Review of Ecology Evolution and Systematics 36 419-444. (Pubitemid 43068865)