Increased DNA amplification success of non-invasive genetic samples by successful removal of inhibitors from faecal samples collected in the field

Conservation Genetics Resources

Volumn 3, Issue 1, 2011, Pages 41-43

  Hebert, Louise ^a   Darden, Safi K ^a,b   Pedersen, Bo Vest ^a   Dabelsteen, Torben ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF EXETER   (United Kingdom)

Author keywords

Chelex; Faecal samples; Inhibitors; Non invasive genetic sampling; Vulpes velox

Indexed keywords

ANIMALIA; VULPES VELOX;

EID: 84857636616     PISSN: 18777252     EISSN: 18777260     Source Type: Journal    
DOI: 10.1007/s12686-010-9280-8     Document Type: Article

References (14)

1. Creel S, Spong G, Sands JL, Rotella J, Zeigle J, Joe L, Murphy KM, Smith D (2003) Population size estimation in Yellowstone wolves with error-prone noninvasive microsatellite genotypes. Mol Ecol 12: 2003-2009.
2. Frantz AC, Pope LC, Carpenter PJ, Roper TJ, Wilson GJ, Delahay RJ, Burke T (2003) Reliable microsatellite genotyping of the eurasian badger (Meles meles) using faecal DNA. Mol Ecol 12: 1649-1661.
3. Fredholm M, Winterø AK (1995) Variation of short tandem repeats within and between species belonging to the canidae family. Mamm Genome 6: 11-18.
4. Gonzalez S, Maldonado JE, Ortega J, Talarico AC, Bidegaray-Batista L, Garcia JE, Duarte JMB (2009) Identification of the endangered small red brocket deer (Mazama bororo) using noninvasive genetic techniques (Mammalia; Cervidae). Mol Ecol 9: 754-758.
5. Hedmark E, Flagstad Ø, Segerström P, Persson J, Landa A, Ellegren H (2004) DNA-based individual and sex identification from wolverine (Gulo gulo) faeces and urine. Conserv Genet 5: 405-410.
6. Idaghdour Y, Broderick D, Korrida A (2003) Faeces as a source of DNA for molecular studies in a threatened population of great bustards. Conserv Genet 4: 789-792.
7. Kitchen AM, Gese EM, Waits LP, Karki SM, Schauster ER (2005) Genetic and spatial structure within a swift fox population. J Anim Ecol 74: 1173-1181.
8. Lantz PG, Matsson M, Wadstrom T, Radstrom P (1997) Removal of PCR inhibitors from human faecal samples through the use of an aqueous two-phase system for sample preparation prior to PCR. J Microbiol Methods 28: 159-167.
9. McKelvey KS, Schwartz MK (2004) Genetic errors associated with population estimation using non-invasive molecular tagging: problems and new solutions. J Wildl Manag 68: 439-448.
10. Monteiro L, Bonnemaison D, Vekris A, Petry KG, Bonnet J, Vidal R, Cabrita J, Megraud F (1997) Complex polysaccharides as PCR inhibitors in feces: helicobacter pylori model. J Clin Microbiol 35: 995-998.
11. Pérez T, Vázquez F, Naves J, Fernández A, Corao A, Albornoz J, Domínguez A (2008) Non-invasive genetic study of the endangered cantabrian brown bear (Ursus arctos). Conserv Genet 10: 291-301.
12. Quéméré E, Louis Jr EE, Ribéron A, Chikhi L, Cronuau-Roy B (2009) Non-invasive conservation genetics of the critically endangered golden-crowned sifaka (Propithecus tattersalli): high diversity and significant genetic differentiation over a small range. Conserv Gene. doi:10.1007/s10592-009-9837-9.
13. Smith DA, Ralls K, Hurt A, Adams B, Parker M, Maldonado JE (2006) Assessing reliability of microsatellite genotypes from kit fox faecal samples using genetic and GIS analyses. Mol Ecol 15: 387-406.
14. Waits LP, Paetkau D (2005) Noninvasive genetic sampling tools for wildlife biologists: a review of applications and recommendations for accurate data collection. J Wildl Manag 69: 1419-1433.