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Volumn 39, Issue , 2012, Pages 11-17

PCR-RFLP detection of the E198A mutation conferring resistance to benzimidazoles in field isolates of Monilinia laxa from Greece

Author keywords

tubulin mutation; Diethofencarb; Monilinia laxa; Monitoring resistance; PCR RFLP; Zoxamide

Indexed keywords

FITNESS; FUNGICIDE; FUNGUS; GENETICS; MUTATION; PATHOGEN; PESTICIDE RESISTANCE; PHENOTYPE; POLYMERASE CHAIN REACTION; TOXICITY TEST;

EID: 84861009944     PISSN: 02612194     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cropro.2012.04.001     Document Type: Article
Times cited : (36)

References (32)
  • 1
    • 84862651160 scopus 로고    scopus 로고
    • Brown rot of stone fruit
    • Elsevier Academic, UK
    • Agrios G. Brown rot of stone fruit. Plant Pathology 2005, 509-510. Elsevier Academic, UK. fifth ed.
    • (2005) Plant Pathology , pp. 509-510
    • Agrios, G.1
  • 2
    • 0032815945 scopus 로고    scopus 로고
    • Mutations of the β-tubulin gene associated with different phenotypes of benzimidazole resistance in the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis
    • Albertini C., Gredt M., Leroux P. Mutations of the β-tubulin gene associated with different phenotypes of benzimidazole resistance in the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis. Pestic. Biochem. Physiol. 1999, 97:17-31.
    • (1999) Pestic. Biochem. Physiol. , vol.97 , pp. 17-31
    • Albertini, C.1    Gredt, M.2    Leroux, P.3
  • 3
    • 42149107430 scopus 로고    scopus 로고
    • Genotyping of benzimidazole-resistant and dicarboximide-resistant mutations in Botrytis cinerea using real-time polymerase chain reaction assays
    • Banno S., Fukumori F., Ichiishi A., Okada K., Uekusa H., Kimura M., Fujimura M. Genotyping of benzimidazole-resistant and dicarboximide-resistant mutations in Botrytis cinerea using real-time polymerase chain reaction assays. Phytopathology 2008, 98:397-404.
    • (2008) Phytopathology , vol.98 , pp. 397-404
    • Banno, S.1    Fukumori, F.2    Ichiishi, A.3    Okada, K.4    Uekusa, H.5    Kimura, M.6    Fujimura, M.7
  • 6
    • 68249149167 scopus 로고    scopus 로고
    • Thiabendazole resistance and mutations in the beta-tubulin gene of Penicillium expansum strains isolated from apples and pears with blue mold decay
    • Cabanas R., Castella G., Abarca M., Bragulat M., Cabanes F. Thiabendazole resistance and mutations in the beta-tubulin gene of Penicillium expansum strains isolated from apples and pears with blue mold decay. Pestic. Biochem. Physiol. 2009, 297:189-195.
    • (2009) Pestic. Biochem. Physiol. , vol.297 , pp. 189-195
    • Cabanas, R.1    Castella, G.2    Abarca, M.3    Bragulat, M.4    Cabanes, F.5
  • 7
    • 0000336395 scopus 로고
    • Sensitivity of Neurospora crassa to benzimidazoles and N-phenylcarbamates: effect of amino acid substitutions at position 198 in β tubulin
    • Fujimura M., Kamakura T., Inoue S., Yamaguchi I. Sensitivity of Neurospora crassa to benzimidazoles and N-phenylcarbamates: effect of amino acid substitutions at position 198 in β tubulin. Pestic. Biochem. Physiol. 1992, 44:165-167.
    • (1992) Pestic. Biochem. Physiol. , vol.44 , pp. 165-167
    • Fujimura, M.1    Kamakura, T.2    Inoue, S.3    Yamaguchi, I.4
  • 8
    • 33749301371 scopus 로고
    • Genetic variability in the fungi and the problem of fungicide resistance
    • Georgopoulos S., Skylakakis G. Genetic variability in the fungi and the problem of fungicide resistance. Crop Prot. 1986, 5:299-305.
    • (1986) Crop Prot. , vol.5 , pp. 299-305
    • Georgopoulos, S.1    Skylakakis, G.2
  • 9
    • 0026708525 scopus 로고
    • Amino acid alterations in the benA (Beta-tubulin) gene of Aspergillus nidulans that confer benomyl resistance
    • Jung M., Wilder I., Oakley B. Amino acid alterations in the benA (Beta-tubulin) gene of Aspergillus nidulans that confer benomyl resistance. Cell Motil. Cytoskeleton 1992, 22:170-174.
    • (1992) Cell Motil. Cytoskeleton , vol.22 , pp. 170-174
    • Jung, M.1    Wilder, I.2    Oakley, B.3
  • 10
    • 0242389010 scopus 로고
    • Negatively correlated cross-resistance between benzimidazole fungicides and methyl N-(3,5-dichlorephenyl)-carbamate
    • Kato T., Suzuki J., Takahashi K., Kamoshita K. Negatively correlated cross-resistance between benzimidazole fungicides and methyl N-(3,5-dichlorephenyl)-carbamate. J. Pest Sci. 1984, 9:489-495.
    • (1984) J. Pest Sci. , vol.9 , pp. 489-495
    • Kato, T.1    Suzuki, J.2    Takahashi, K.3    Kamoshita, K.4
  • 11
    • 0000556725 scopus 로고
    • Characterization of mutations in the beta-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi
    • Koenraadt H., Somerville S., Jones A. Characterization of mutations in the beta-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi. Phytopathology 1992, 82:1348-1354.
    • (1992) Phytopathology , vol.82 , pp. 1348-1354
    • Koenraadt, H.1    Somerville, S.2    Jones, A.3
  • 12
    • 0000383305 scopus 로고    scopus 로고
    • Occurrence of Botrytis cinerea Pers. ex Fr. strains with coupling resistance to benzimidazoles and phenylcarbamates in protected crops in Greece
    • Laskaris D., Pappas A., Kyriakopoulos C. Occurrence of Botrytis cinerea Pers. ex Fr. strains with coupling resistance to benzimidazoles and phenylcarbamates in protected crops in Greece. Phytopathol. Mediterr. 1996, 35:223.
    • (1996) Phytopathol. Mediterr. , vol.35 , pp. 223
    • Laskaris, D.1    Pappas, A.2    Kyriakopoulos, C.3
  • 13
    • 0001416679 scopus 로고
    • Negative cross-resistance of benzimidazole resistant strains of Botrytis cinerea, Fusarium nivale and Pseudocercosporella herpotrichoides to various pesticides
    • Leroux P., Gredt M. Negative cross-resistance of benzimidazole resistant strains of Botrytis cinerea, Fusarium nivale and Pseudocercosporella herpotrichoides to various pesticides. Neth. J. Plant Pathol. 1989, 95:121-127.
    • (1989) Neth. J. Plant Pathol. , vol.95 , pp. 121-127
    • Leroux, P.1    Gredt, M.2
  • 14
    • 0036729267 scopus 로고    scopus 로고
    • Mechanisms of resistance to fungicides in field strains of Botrytis cinerea
    • Leroux P., Fritz R., Debieu D., Albertini C., Lanen C., Bach J., et al. Mechanisms of resistance to fungicides in field strains of Botrytis cinerea. Pest Manag. Sci. 2002, 58:876-888.
    • (2002) Pest Manag. Sci. , vol.58 , pp. 876-888
    • Leroux, P.1    Fritz, R.2    Debieu, D.3    Albertini, C.4    Lanen, C.5    Bach, J.6
  • 15
    • 36348971266 scopus 로고    scopus 로고
    • Quantification of allele E198A in beta-tubulin conferring benzimidazole resistance in Monilinia fructicola using real-time PCR
    • Luo Y., Ma Z., Michailides T. Quantification of allele E198A in beta-tubulin conferring benzimidazole resistance in Monilinia fructicola using real-time PCR. Pest Manag. Sci. 2007, 63:1178-1184.
    • (2007) Pest Manag. Sci. , vol.63 , pp. 1178-1184
    • Luo, Y.1    Ma, Z.2    Michailides, T.3
  • 16
    • 23044464155 scopus 로고    scopus 로고
    • Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi
    • Ma Z., Michailides T. Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi. Crop Prot. 2005, 24:853-863.
    • (2005) Crop Prot. , vol.24 , pp. 853-863
    • Ma, Z.1    Michailides, T.2
  • 17
    • 2142804689 scopus 로고    scopus 로고
    • Identification and characterization of benzimidazole resistance in Monilinia fructicola from stone fruit orchands in California
    • Ma Z., Yoshimura M., Michailides T. Identification and characterization of benzimidazole resistance in Monilinia fructicola from stone fruit orchands in California. Appl. Environ. Microbiol. 2003, 69:7145-7152.
    • (2003) Appl. Environ. Microbiol. , vol.69 , pp. 7145-7152
    • Ma, Z.1    Yoshimura, M.2    Michailides, T.3
  • 18
    • 17644367530 scopus 로고    scopus 로고
    • Characterization and PCR-based detection of benzimidazole-resistant isolates of Monilinia laxa in California
    • Ma Z., Yoshimura M., Holtz B., Michailides T. Characterization and PCR-based detection of benzimidazole-resistant isolates of Monilinia laxa in California. Pest Manag. Sci. 2005, 61:449-452.
    • (2005) Pest Manag. Sci. , vol.61 , pp. 449-452
    • Ma, Z.1    Yoshimura, M.2    Holtz, B.3    Michailides, T.4
  • 19
    • 78650878662 scopus 로고    scopus 로고
    • Molecular characterization of benzimidazole-resistant B. cinerea field isolates with reduced or enhanced sensitivity to zoxamide and diethofencarb
    • Malandrakis A., Markoglou A., Ziogas B. Molecular characterization of benzimidazole-resistant B. cinerea field isolates with reduced or enhanced sensitivity to zoxamide and diethofencarb. Pestic. Biochem. Physiol. 2011, 99:118-124.
    • (2011) Pestic. Biochem. Physiol. , vol.99 , pp. 118-124
    • Malandrakis, A.1    Markoglou, A.2    Ziogas, B.3
  • 20
    • 79953162276 scopus 로고    scopus 로고
    • Molecular diagnostic for detecting the cytochrome b G143S - QoI resistance mutation in Cercospora beticola
    • Malandrakis A., Markoglou A., Nikou D., Vontas J., Ziogas B. Molecular diagnostic for detecting the cytochrome b G143S - QoI resistance mutation in Cercospora beticola. Pestic. Biochem. Physiol. 2011, 100:87-92.
    • (2011) Pestic. Biochem. Physiol. , vol.100 , pp. 87-92
    • Malandrakis, A.1    Markoglou, A.2    Nikou, D.3    Vontas, J.4    Ziogas, B.5
  • 21
    • 0001954236 scopus 로고
    • Studies on gray mold (Botrytis cinerea) of vegetables grown under plastics
    • Malathrakis N. Studies on gray mold (Botrytis cinerea) of vegetables grown under plastics. Phytopathol. Mediterr. 1979, 19:70.
    • (1979) Phytopathol. Mediterr. , vol.19 , pp. 70
    • Malathrakis, N.1
  • 22
    • 79959187061 scopus 로고    scopus 로고
    • Sensitivity of Monilinia fructicola from brazil to tebuconazole, azoxystrobin, and thiophanate-methyl and implications for disease management
    • May-De Mio L., Luo Y., Michailides T. Sensitivity of Monilinia fructicola from brazil to tebuconazole, azoxystrobin, and thiophanate-methyl and implications for disease management. Plant Dis. 2011, 95:821-827.
    • (2011) Plant Dis. , vol.95 , pp. 821-827
    • May-De Mio, L.1    Luo, Y.2    Michailides, T.3
  • 23
    • 0031815182 scopus 로고    scopus 로고
    • Identification of benzimidazole resistance in Cladobotryum dendroides using a PCR-based method
    • McKay G., Egan D., Morris E., Brown A. Identification of benzimidazole resistance in Cladobotryum dendroides using a PCR-based method. Mycol. Res. 1998, 102:671-676.
    • (1998) Mycol. Res. , vol.102 , pp. 671-676
    • McKay, G.1    Egan, D.2    Morris, E.3    Brown, A.4
  • 24
    • 77954330419 scopus 로고    scopus 로고
    • Monilinia laxa, M. fructigena and M. fructicola: Risk estimation of resistance to QoI fungicides and identification of species with cytochrome b gene sequences
    • Miessner S., Stammler G. Monilinia laxa, M. fructigena and M. fructicola: Risk estimation of resistance to QoI fungicides and identification of species with cytochrome b gene sequences. J. Plant Dis. Prot. 2010, 117:162-167.
    • (2010) J. Plant Dis. Prot. , vol.117 , pp. 162-167
    • Miessner, S.1    Stammler, G.2
  • 25
    • 84862700000 scopus 로고    scopus 로고
    • OEPP/EPPO (European and Mediterranean Plant Protection Organization)
    • Available from:, (last accessed 17.03.11.)
    • OEPP/EPPO (European and Mediterranean Plant Protection Organization) Data Sheets on Quarantine Organisms No. 153, Monilinia fructicola 1997, 5 pp. Available from:, (last accessed 17.03.11.). http://www.eppo.org/QUARANTINE/fungi/Monilinia_fructicola/MONIFC_ds.pdf.
    • (1997) Data Sheets on Quarantine Organisms No. 153, Monilinia fructicola , pp. 5
  • 26
    • 0022751236 scopus 로고
    • Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker
    • Orbach M., Porro E., Yanofsky C. Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol. Cell. Biol. 1986, 6:2452-2461.
    • (1986) Mol. Cell. Biol. , vol.6 , pp. 2452-2461
    • Orbach, M.1    Porro, E.2    Yanofsky, C.3
  • 27
    • 38249020240 scopus 로고
    • Prolonged field persistence of resistance to benomyl in Monilinia fructicola
    • Penrose L. Prolonged field persistence of resistance to benomyl in Monilinia fructicola. Crop Prot. 1990, 9:190-192.
    • (1990) Crop Prot. , vol.9 , pp. 190-192
    • Penrose, L.1
  • 28
    • 59149101590 scopus 로고    scopus 로고
    • Contribution of pathogens to peach fruit rot in northern Greece and their sensitivity to iprodione, carbendazim, thiophanate-methyl and tebuconazole fungicides
    • Thomidis T., Michailides T., Exadaktylou E. Contribution of pathogens to peach fruit rot in northern Greece and their sensitivity to iprodione, carbendazim, thiophanate-methyl and tebuconazole fungicides. J. Phytopathol. 2009, 157:194-200.
    • (2009) J. Phytopathol. , vol.157 , pp. 194-200
    • Thomidis, T.1    Michailides, T.2    Exadaktylou, E.3
  • 29
    • 20044377107 scopus 로고    scopus 로고
    • Development of a high-throughput method for quantification of Plasmopara viticola DNA in grapevine leaves by means of quantitative real-time polymerase chain reaction
    • Valsesia G., Gobbin D., Patocchi A., Vecchione A., Pertot I., Gessler C. Development of a high-throughput method for quantification of Plasmopara viticola DNA in grapevine leaves by means of quantitative real-time polymerase chain reaction. Phytopathology 2005, 95:672-678.
    • (2005) Phytopathology , vol.95 , pp. 672-678
    • Valsesia, G.1    Gobbin, D.2    Patocchi, A.3    Vecchione, A.4    Pertot, I.5    Gessler, C.6
  • 30
    • 0027139615 scopus 로고
    • Mutations leading to substitutions at amino acid 198 and 200 of beta-tubulin that correlate with benomyl-resistant phenotypes of field strains of Botrytis cinerea
    • Yarden O., Katan T. Mutations leading to substitutions at amino acid 198 and 200 of beta-tubulin that correlate with benomyl-resistant phenotypes of field strains of Botrytis cinerea. Phytopathology 1993, 83:1478-1483.
    • (1993) Phytopathology , vol.83 , pp. 1478-1483
    • Yarden, O.1    Katan, T.2
  • 31
    • 77953860482 scopus 로고    scopus 로고
    • Cross resistance relationships between zoxamide, carbendazim and diethofencarb in field isolates of Botrytis cinerea and other fungi
    • Young D., Slawecki R. Cross resistance relationships between zoxamide, carbendazim and diethofencarb in field isolates of Botrytis cinerea and other fungi. Modern Fungicides and Antifungal Compounds IV 2005, 125-131.
    • (2005) Modern Fungicides and Antifungal Compounds IV , pp. 125-131
    • Young, D.1    Slawecki, R.2
  • 32
    • 70349671186 scopus 로고    scopus 로고
    • Identification of a novel point mutation in the beta-tubulin gene of Botrytis cinerea and detection of benzimidazole resistance by a diagnostic PCR-RFLP assay
    • Ziogas B., Nikou D., Markoglou A., Malandrakis A., Vontas J. Identification of a novel point mutation in the beta-tubulin gene of Botrytis cinerea and detection of benzimidazole resistance by a diagnostic PCR-RFLP assay. Eur. J. Plant Pathol. 2009, 125:97-107.
    • (2009) Eur. J. Plant Pathol. , vol.125 , pp. 97-107
    • Ziogas, B.1    Nikou, D.2    Markoglou, A.3    Malandrakis, A.4    Vontas, J.5


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