Development of a high-throughput method for quantification of Plasmopara viticola DNA in grapevine leaves by means of quantitative real-time polymerase chain reaction

Phytopathology

Volumn 95, Issue 6, 2005, Pages 672-678

  Valsesia, G ^a   Gobbin, D ^a   Patocchi, A ^a   Vecchione, A ^b   Pertot, I ^b   Gessler, C ^a  

^a ETH ZURICH   (Switzerland)

^b RESEARCH AND INNOVATION CENTRE   (Italy)

Author keywords

Biocontrol agents; Downy mildew; TaqMan

Indexed keywords

PERONOSPORA FARINOSA; PLASMOPARA VITICOLA; VITIS; VITIS SP.; VITIS VINIFERA;

EID: 20044377107     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO-95-0672     Document Type: Article

References (17)

1. Böhm, J., Hahn, A., Schubert, R., Bahnweg, G., Adler, N., Nechwatal, J., Oehlmann, R., and Osswald, W. 1999. Real-time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophthora infestans and Phytophthora citricola in their respective host plants. J. Phytopathol. 147:409-416.
2. Ennos, R. A. 2000. The introduction of lodgepole pine as a major forest crop in Sweden: Implications for host-pathogen evolution. For. Ecol. Manag. 141:85-96.
3. Gobbin, D., Pertot, I., and Gessler, C. 2003. Identification of microsatellite markers for Plasmopara viticola and establishment of high throughput method for SSR analysis. Eur. J. Phytopathol. 109:153-164.
4. Hain, R., Reif, H. J., Krause, E., Langebartels, R., Kindl, H., Vornam, B., Wiese, W., Schmelzer, E., Schreier, P. H., Stocker, R. H., and Stenzel, K. 1993. Disease resistance results from foreign phytoalexin expression in a novel plant. Nature 361:153-156.
5. Jermini, M., Blaise, Ph., and Gessler, C. 1997. Preliminary investigations on the impact of Plasmopara viticola on yield quantity and quality of Vitis vinifera. Die Weinwissenschaft 52:156.
6. Lie, Y. S., and Petropoulos, C. J. 1998. Advances in quantitative PCR technology: 5′ nuclease assays. Curr. Opin. Biotechnol. 9:43-48.
7. Lodhi, M. A., Guang-Ning, Y., Weeden, N. F., and Reisch, B. I. 1994. A simple and efficient method for DNA extraction from V. vinifera cultivars and Vitis species. Plant Mol. Biol. Rep. 12:6-13.
8. Mumford, R. A., Walsh, K., Barker, I., and Boonham, N. 2000. Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology 90:448-453.
9. Oliveira, A. C., Vallim, M. A., Semighini, C. P., Araújo, L., Goldman, G. H., and Machado, M. A. 2002. Quantification of Xylella fastidiosa from citrus trees by real-time polymerase reaction assay. Phytopathology 92:1048-1054.
10. Qi, M., and Yang, Y. 2002. Quantification of Magnaporthe grisea during infection of rice plants using real-time polymerase chain reaction and northern blot/phosphoimaging analyses. Phytopathology 92:870-876.
11. Roberts, C. A., Dietzgen, R. G., Heelan, L. A., and Maclean, D. J. 2000. Real time PCR fluorescent detection of Tomato spotted wilt virus. J. Virol. Methods 88:1-8.
12. Rohel, E. A., Laurent, P., Fraaije, B., Cavelier, N., and Hollomon, D. W. 2002. Quantitative PCR monitoring of the effect of azoxystrobin treatments on Mycosphaerella graminicola epidemics in the field. Pestic. Manag. Sci. 58:248-254.
13. Schaad, N. W., Opgenorth, D., and Gaush, P. 2002. Real-time polymerase chain reaction for one-hour on-site diagnosis of Pierce's disease of grape in early season asymptomatic vines. Phytopathology 92:721-728.
14. Taylor, E., Bates, J., Kenyon, D., Maccaferri, M., and Thomas, J. 2001. Modern molecular methods for characterization and diagnosis of seed-borne fungal pathogens. J. Plant Pathol. 83:75-81.
15. Thompson, J. D., Higgins, D. G., and Gibson, T. J. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
16. Winton, L. M., Stone, J. K., Watrud, L. S., and Hansen, E. M. 2002. Simultaneous one-tube quantification of host and pathogen DNA with real-time polymerase chain reaction. Phytopathology 92:112-116.
17. Wong, F. P., Burr, H. N., and Wilcox, W. F. 2001. Heterothallism in Plasmopara viticola. Plant Pathol. 50:427-432.