Genotyping of benzimidazole-resistant and dicarboximide-resistant mutations in Botrytis cinerea using real-time polymerase chain reaction assays

Phytopathology

Volumn 98, Issue 4, 2008, Pages 397-404

  Banno, Shinpei ^a   Fukumori, Fumiyasu ^a   Ichiishi, Akihiko ^a   Okada, Kiyotsugu ^b   Uekusa, Hidetoshi ^c   Kimura, Makoto ^d   Fujimura, Makoto ^a  

^a TOYO UNIVERSITY   (Japan)

^b Agricultural Food and Environmental Sciences Research Center of Osaka Prefecture   (Japan)

^c Kanagawa Prefectural Agricultural Research Institute   (Japan)

^d Japan Science and Technology Agency   (Japan)

Author keywords

Botryotinia fuckeliana

Indexed keywords

BENZIMIDAZOLE; BENZIMIDAZOLE DERIVATIVE; FUNGAL DNA; FUNGICIDE; IMIDE;

ARTICLE; BOTRYTIS; CUCUMBER; GENETICS; GENOTYPE; MICROBIOLOGY; MULTIDRUG RESISTANCE; MUTATION; NUCLEOTIDE SEQUENCE; PLANT DISEASE; PLANT LEAF; POLYMERASE CHAIN REACTION;

BASE SEQUENCE; BENZIMIDAZOLES; BOTRYTIS; CUCUMIS SATIVUS; DNA, FUNGAL; DRUG RESISTANCE, MULTIPLE, FUNGAL; FUNGICIDES, INDUSTRIAL; GENOTYPE; IMIDES; MUTATION; PLANT DISEASES; PLANT LEAVES; POLYMERASE CHAIN REACTION;

BOTRYOTINIA FUCKELIANA; BOTRYTIS;

EID: 42149107430     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO-98-4-0397     Document Type: Article

References (29)

1. Altertini, C., Gredt, M., and Leroux, P. 1999. Mutations of the β-tubulin gene associated with different phenotypes of benzimidazole resistance in the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis. Pestic. Biochem. Physiol. 64:17-31.
2. Banno, S., Noguchi, R., Yamashita, K., Fukumori, F., Kimura, M., Yamaguchi, I., and Fujimura, M. 2007. Roles of putative His-to-Asp signaling modules HPT-1 and RRG-2, on viability and sensitivity to osmotic and oxidative stresses in Neurospora crassa. Curr. Genet. 51:197-208.
3. Beever, R. E., Laracy, E. P., and Pak, H. A. 1989. Strains of Botrytis cinerea resistant to dicarboximide and benzimidazole fungicides in New Zealand vineyards. Plant Pathol. 38:427-437.
4. Carattoli, A., Dionisi, A., and Luzzi, I. 2002. Use of a LightCycler gyrA mutation assay for identification of ciprofloxacin-resistant Campylobacter coli. FEMS Microbiol. Lett. 214:87-93.
5. Cui, W., Beever, R. E., Parkes, S. L., and Templeton, M. D. 2004. Evolution of an osmosensing histidine kinase in field strains of Botryotinia fuckeliana (Botrytis cinerea) in response to dicarboximide fungicide usage. Phytopathology 94:1129-1135.
6. Davidse, L. C., and Flach, W. 1978. Interaction of thiabendazole with fungal tubulin. Biochim. Biophys. Acta 543:82-90.
7. Fujimura, M., Kamakura, T., Inoue, H., and Yamaguchi, I. 1994. Aminoacid alterations in the beta-tubulin gene of Neurospora crassa that confer resistance to carbendazim and diethofencarb. Curr. Genet. 25:418-422.
8. Fujimura, M., Kamakura, T., Yamaguchi, I. 1994. Mode of action of diethofencarb to benzimidazole-resistant mutant in Neurospora crassa. J. Pestic. Sci. 17:237-242.
9. Fujimura, M., Ochiai, N., Motoyama, T., and Yamaguchi, I. 2002. Mechanism of resistance to dicarboximides and phenylpyrrols. Pages 200-214 in: Agrochemical Resistance; Extent, Mechanism, and Detection. J. M. Clark and I. Yamaguchi, ed. American Chemical Society, Washington, DC.
10. Fujimura, M., Ochiai, N., Oshima, M., Motoyama, T., Ichiishi, A., Usami, R., Horikoshi, K., and Yamaguchi, I. 2003. Putative homologs of SSK22 MAPKK kinase and PBS2 MAPK kinase of Saccharomyces cerevisiae encoded by os-4 and os-5 genes for osmotic sensitivity and fungicide resistance in Neurospora crassa. Biosci. Biotechnol. Biochem. 67:186-191.
11. Fujimura, M., Oeda, K., Inoue, H., and Kato, T. 1992. A single aminoacid substitution in the beta-tubulin gene of Neurospora confers both carbendazim resistance and diethofencarb sensitivity. Curr. Genet. 21:399-404.
12. Ishii, H. 2006. Impact of fungicide resistance in plant pathogens on crop disease control and agricultural environment. JARQ 40:205-211.
13. Kaku, K., Takagaki, M., Shimizu, T., and Nagayama, K. 2003. Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI-D) resistance by primer-introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea. Pest Manag. Sci. 59:843-846.
14. Koenraadt, H., Somerville, S. C., and Jones, A. L. 1992. Characterization of mutations in the beta-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi. Phytopathology 82:1348-1354.
15. Leroux, P., Fritz, R., Debieu, D., Albertini, C., Lanen, C., Bach, J., Gredt, M., and Chapeland, F. 2002. Mechanisms of resistance to fungicides in field strains of Botrytis cinerea. Pest Manag. Sci. 58:876-888.
16. Loeffler, J., Hagmeyer, L., Hebart, H., Henke, N., Schumacher, U., and Einsele, H. 2000. Rapid detection of point mutations by fluorescence resonance energy transfer and probe melting curves in Candida species. Clin. Chem. 46:631-635.
17. Luck, J. E., and Gillings, M. R. 1995. Rapid identification of benomyl resistant strains of Botrytis cinerea using the polymerase chain reaction. Mycol. Res. 99:1483-1488.
18. Ma, Z., and Michailides, T. J. 2005. Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi. Crop Prot. 24:853-863.
19. Ma, Z., Yan, L., Luo, Y., and Michailides, T. J. 2007. Sequence variation in the two-component histidine kinase gene of Botrytis cinerea associated with resistance to dicarboximide fungicides. Pestic. Biochem. Physiol. 88:300-306.
20. Maymon, M., Zveibil, A., Pivonia, S., Minz, D., and Freeman, S. 2006. Identification and characterization of benomyl-resistant and -sensitive populations of Colletotrichum gloeosporioides from Statice (Limonium spp.). Phytopathology 96:542-548.
21. Motoyama, T., Ohira, T., Kadokura, K., Ichiishi, A., Fujimura, M., Yamaguchi, I., and Kudo, T. 2005. An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast. Curr. Genet. 47:298-306.
22. Noguchi, R., Banno, S., Ichikawa, R., Fukumori, F., Ichiishi, A., Kimura, M., Yamaguchi, I., and Fujimura, M. 2007. Identification of OS-2 MAP kinase-dependent genes induced in response to osmotic stress, antifungal agent fludioxonil, and heat shock in Neurospora crassa. Fungal Genet. Biol. 44:208-218.
23. Ochiai, N., Fujimura, M., Oshima, M., Motoyama, T., Ichiishi, A., Yamada-Okabe, H., and Yamaguchi, I. 2002. Effects of iprodione and fludioxonil on glycerol synthesis and hyphal development in Candida albicans. Biosci. Biotechnol. Biochem. 66:2209-2215.
24. Oleastro, M., Menard, A., Santos, A., Lamouliatte, H., Monteiro, L., Barthelemy, P., and Megraud, F. 2003. Real-time PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori. J. Clin. Microbiol. 41:397-402.
25. Oshima, M., Banno, S., Okada, K., Takeuchi, T., Kimura, M., Ichiishi, A., Yamaguchi, I., and Fujimura, M. 2006. Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea. J. Gen. Plant Pathol. 72:65-73.
26. Oshima, M., Fujimura, M., Banno, S., Hashimoto, C., Motoyama, T., Ichiishi, A., and Yamaguchi, I. 2002. A point mutation in the two-component histidine kinase BcOS-1 gene confers dicarboximide resistance in field isolates of Botrytis cinerea. Phytopathology 92:75-80.
27. Yarden, O., and Katan, T. 1993. Mutations leading to substitutions at amino acids 198 and 200 of beta-tubulin that correlate with benomylresistance phenotypes of field strains of Botrytis cinerea. Phytopathology 83:1478-1483.
28. Yourman, L. F., and Jeffers, S. N. 1999. Resistance to benzimidazole and dicarboximide fungicides in greenhouse isolates of Botrytis cinerea. Plant Dis. 83:569-575.
29. Yourman, L. F., Jeffers, S. N., and Dean, R. A. 2000. Genetic analysis of isolates of Botrytis cinerea sensitive and resistant to benzimidazole and dicarboximide fungicides. Phytopathology 90:851-859.