The neuroblast assay: An assay for the generation and enrichment of neuronal progenitor cells from differentiating neural stem cell progeny using flow cytometry

Journal of Visualized Experiments

Volumn , Issue 62, 2012, Pages

  Azari, Hassan ^a,b   Sharififar, Sharareh ^a   Fortin, Jeff M ^a   Reynolds, Brent A ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b SHIRAZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Enrichment; Flow Cytometry; Isolation; Issue 62; Neural Stem Cell; Neuronal Progenitor Cells; Neuroscience

Indexed keywords

EID: 84860627915     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3712     Document Type: Article

References (6)

1. Azari, H., Rahman, M., Sharififar, S., & Reynolds, B. Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay. J. Vis. Exp. (45), e2393, DOI: 10.3791/2393 (2010).
2. Azari, H., Sharififar, S., Rahman, M., Ansari, S., & Reynolds, B. Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay. J. Vis. Exp. (47), e2457, DOI: 10.3791/2457 (2011).
3. Siebzehnrubl, F.A., Vedam-Mai, V., Azari, H., Reynolds, B.A., & Deleyrolle, L.P., Isolation and Characterization of Adult Neural Stem Cells. Methods. Mol. Biol. 750, 61-77 (2011).
4. Scheffler, B., et al. Phenotypic and functional characterization of adult brain neuropoiesis. Proceedings of the National Academy of Sciences of the United States of America. 102 (26), 9353-9358 (2005).
5. Azari, H., et al. Purification of immature neuronal cells from neural stem cell progeny. PLoS One. 6 (6), e20941 (2011).
6. Amariglio, N., et al. Donor-Derived Brain Tumor Following Neural Stem Cell Transplantation in an Ataxia Telangiectasia Patient. Plos Medicine. 6 (2), 221-231 (2009).