Isolation and Characterization of Adult Neural Stem Cells

Methods in Molecular Biology

Volumn 750, Issue , 2011, Pages 61-77

  Siebzehnrubl, Florian A ^b   Vedam Mai, Vinata ^b   Azari, Hassan ^b   Reynolds, Brent A ^b   Deleyrolle, Loic P ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b NONE

Author keywords

Flow cytometry; Immunohistochemistry; Neural colony forming cell assay; Neural stem cell; Neurosphere assay

Indexed keywords

ADULT; BRAIN TISSUE; CELL ASSAY; CELL SELECTION; CENTRAL NERVOUS SYSTEM; COLONY FORMING CELL; FLOW CYTOMETRY; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; NEURAL STEM CELL; STEM CELL CULTURE; ADULT STEM CELL; ANIMAL; ARTICLE; CELL DIFFERENTIATION; CELL SEPARATION; CLONOGENIC ASSAY; CULTURE TECHNIQUE; CYTOLOGY; DENTATE GYRUS; GLIA; HIPPOCAMPUS; HUMAN; METABOLISM; METHODOLOGY; MOUSE; NERVE CELL; PHYSIOLOGY;

ADULT; ADULT STEM CELLS; ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL SEPARATION; COLONY-FORMING UNITS ASSAY; DENTATE GYRUS; FLOW CYTOMETRY; HIPPOCAMPUS; HUMANS; IMMUNOHISTOCHEMISTRY; MICE; NEURAL STEM CELLS; NEUROGLIA; NEURONS;

EID: 80052630478     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-145-1_4     Document Type: Chapter

References (13)

1. Reynolds, B.A., and Weiss, S. (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system Science 255, 1707–10.
2. Engstrom, C.M., Demers, D., Dooner, M., McAuliffe, C., Benoit, B.O., Stencel, K., Joly, M., Hulspas, R., Reilly, J.L., Savarese, T., Recht, L.D., Ross, A.H., and Quesenberry, P.J. (2002) A method for clonal analysis of epidermal growth factor-responsive neural progenitors J Neurosci Methods 117, 111–21.
3. Gritti, A., Parati, E.A., Cova, L., Frolichsthal, P., Galli, R., Wanke, E., Faravelli, L., Morassutti, D.J., Roisen, F., Nickel, D.D., Vescovi, A.L. (1996) Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor J Neurosci 16, 1091–100.
4. Louis, S.A., Rietze, R.L., Deleyrolle, L., Wagey, R.E., Thomas, T.E., Eaves, A.C., Reynolds, B.A. (2008) Enumeration of neural stem and progenitor cells in the neural colony-forming cell assay Stem Cells 26, 988–96.
5. Pastrana, E., Cheng, L.C., Doetsch, F. (2009) Simultaneous prospective purification of adult subventricular zone neural stem cells and their progeny Proc Natl Acad Sci USA 106, 6387–92.
6. Capela, A., Temple, S. (2002)LeX/ssea-1 is expressed by adult mouse CNS stem cells, identifying them as nonependymal Neuron 35, 865–75.
7. Uchida, N., Buck, D.W., He, D., Reitsma, M.J., Masek, M., Phan, T.V., Tsukamoto, A.S., Gage, F.H., Weissman, I.L. (2000) Direct isolation of human central nervous system stem cells Proc Natl Acad Sci USA 97, 14720–5.
8. Gilyarov, A.V. (2008) Nestin in central nervous system cells Neurosci Behav Physiol 38, 165–9.
9. Sakakibara, S., Nakamura, Y., Satoh, H., Okano, H. (2001) Rna-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS J Neurosci 21, 8091–107.
10. Graham, V., Khudyakov, J., Ellis, P., Pevny, L. (2003) SOX2 functions to maintain neural progenitor identity Neuron 39, 749–65.
11. Kim, M., Morshead, C.M. (2003) Distinct populations of forebrain neural stem and progenitor cells can be isolated using side-population analysis J Neurosci 23, 10703–9.
12. Corti, S., Locatelli, F., Papadimitriou, D., Donadoni, C., Salani, S., Del Bo, R., Strazzer, S., Bresolin, N., Comi, G.P. (2006) Identification of a primitive brain-derived neural stem cell population based on aldehyde dehydrogenase activity Stem Cells 24, 975–85.
13. Reynolds, B.A., Rietze, R.L. (2005) Neural stem cells and neurospheres--re-evaluating the relationship Nat Methods 2, 333–6.