Identification of functionally relevant populations in enhanced biological phosphorus removal processes based on intracellular polymers profiles and insights into the metabolic diversity and heterogeneity

Environmental Science and Technology

Volumn 46, Issue 9, 2012, Pages 5010-5017

  Majed, Nehreen ^a   Chernenko, Tatyana ^b   Diem, Max ^b   Gu, April Z ^a  

^a Northeastern University   (United States)

^b NORTHEASTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BULK MEASUREMENT; CELLULAR LEVELS; ENERGY GENERATIONS; ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL; FLUORESCENCE IN SITU HYBRIDIZATION; GLYCOGEN ACCUMULATING ORGANISMS; MIXED CULTURES; POLYHYDROXYBUTYRATE; POLYMER INCLUSIONS; POLYPHOSPHATE-ACCUMULATING ORGANISMS; POLYPHOSPHATES; RAMAN METHODS; REDUCING POWER; SIMULTANEOUS DETECTION; SINGLE CELLS; STATE-BASED; TCA CYCLE;

CELL CULTURE; FLUORESCENCE MICROSCOPY; METABOLISM; PATHOLOGY; RAMAN SPECTROSCOPY;

POLYMERS;

GLYCOGEN; PHOSPHORUS; POLYHYDROXYBUTYRATE; POLYMER; POLYPHOSPHATE; UNCLASSIFIED DRUG;

HETEROGENEITY; HYPOTHESIS TESTING; IN SITU MEASUREMENT; METABOLISM; PHOSPHORUS; POLYMER; POWER GENERATION; RAMAN SPECTROSCOPY;

ARTICLE; BIOACCUMULATION; CITRIC ACID CYCLE; ENHANCED BIOLOGICAL PHOSPHORUS REMOVAL; FLUORESCENCE IN SITU HYBRIDIZATION; GLYCOGEN ACCUMULATING ORGANISM; MICROSCOPY; NONHUMAN; ORGANISMS; POLYMERIZATION; POLYPHOSPHATE ACCUMULATING ORGANISM; RAMAN SPECTROMETRY; WASTE WATER; WASTE WATER MANAGEMENT;

GLYCOGEN; MICROBIOLOGICAL TECHNIQUES; PHOSPHORUS; POLYMERS; POLYPHOSPHATES; SPECTRUM ANALYSIS, RAMAN; WATER POLLUTION, CHEMICAL;

EID: 84860491241     PISSN: 0013936X     EISSN: 15205851     Source Type: Journal    
DOI: 10.1021/es300044h     Document Type: Article

References (43)

1. Mino, T.; Van Loosdrecht, M. C. M.; Heijnen, J. J. Microbiology and biochemistry of the enhanced biological phosphate removal process Water Res. 1998, 32 (11) 3193-3207
2. Pereira, H.; Lemos, P. C.; Reis, M. A. M.; Crespo, J. P. S. G.; Carrondo, M. J. T.; Santos, H. Model for carbon metabolism in biological phosphorus removal processes based on in vivo C-13-NMR labelling experiments Water Res. 1996, 30 (9) 2128-2138
3. Seviour, R. J.; Mino, T.; Onuki, M. The microbiology of biological phosphorus removal in activated sludge systems FEMS Microbiol. Rev. 2003, 27 (1) 99-127
4. Oehmen, A.; Carvalho, G.; Lopez-Vazquez, C. M.; van Loosdrecht, M. C. M.; Reis, M. A. M. Incorporating microbial ecology into the metabolic modelling of polyphosphate accumulating organisms and glycogen accumulating organisms Water Res. 2010, 44 (17) 4992-5004
5. He, S.; Gu, A. Z.; McMahon, K. D. Progress toward understanding the distribution of Accumulibacter among full-scale enhanced biological phosphorus removal systems Microb. Ecol. 2008, 55 (2) 229-236
6. Oehmen, A.; Lemos, P. C.; Carvalho, G.; Yuan, Z. G.; Keller, J.; Blackall, L. L.; Reis, M. A. M. Advances in enhanced biological phosphorus removal: From micro to macro scale Water Res. 2007, 41 (11) 2271-2300
7. Gu, A. Z.; Saunders, A.; Neethling, J. B.; Stensel, H. D.; Blackall, L. L. Functionally relevant microorganisms to enhanced biological phosphorus removal performance at full-scale wastewater treatment plants in the United States Water Environment Res. 2008, 80 (8) 688-698
8. Streichan, M.; Golecki, J. R.; Schon, G. Polyphosphate-accumulating bacteria from sewage plant with different processes of biological phosphorus removal FEMS Microbiol. Ecol. 1990, 73, 113-124
9. Kawaharasakia, M.; Tanakab, H.; Kanagawaa, T.; Nakamuraa, K. In situ identification of polyphosphate-accumulating bacteria in activated sludge by dual staining with rRNA-targeted oligonucleotide probes and 4′,6- diamidino-2-phenylindol (DAPI) at a polyphosphate-probing concentration Water Res. 1999, 33 (1) 257-265
10. Crocetti, G. R.; Banfield, J. F.; Keller, J.; Bond, P. L.; Blackall, L. L. Glycogen-accumulating organisms in laboratory-scale and full-scale wastewater treatment processes Microbiol.-Sgm 2002, 148, 3353-3364
11. Burow, L. C.; Kong, Y.; Nielsen, J. L.; Blackall, L. L.; Nielsen, P. H. Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes Microbiol.-Sgm 2007, 153, 178-185
12. Kong, Y. H.; Ong, S. L.; Ng, W. J.; Liu, W. T. Diversity and distribution of a deeply branched novel proteobacterial group found in anaerobic-aerobic activated sludge processes Environ. Microbiol. 2002, 4 (11) 753-757
13. Nielsen, P. H.; Andreasen, K.; Lee, N.; Wagner, M. Use of microautoradiography and fluorescent in situ hybridization for characterization of microbial activity in activated sludge Water Sci. Technol. 1999, 39 (1) 1-9
14. Kong, Y. H.; Nielsen, J. L.; Nielsen, P. H. Microautoradiographic study of Rhodocyclus-related polyphosphate accumulating bacteria in full-scale enhanced biological phosphorus removal plants Appl. Environ. Microbiol. 2004, 70 (9) 5383-5390
15. Gunther, S.; Trutnau, M.; Kleinsteuber, S.; Hause, G.; Bley, T.; Roske, I.; Harms, H.; Muller, S. Dynamics of polyphosphate-accumulating bacteria in wastewater treatment plant microbial communities detected via DAPI (4 ',6 '-diamidino-2-phenylindole) and tetracycline labeling Appl. Environ. Microbiol. 2009, 75 (7) 2111-2121
16. Majed, N.; Matthaus, C.; Diem, M.; Gu, A. Z. Evaluation of intracellular polyphosphate dynamics in enhanced biological phosphorus removal process using raman microscopy Environ. Sci. Technol. 2009, 43 (11) 5436-5442
17. Majed, N.; Gu, A. Z. Application of raman microscopy for simultaneous and quantitative evaluation of multiple intracellular polymers dynamics functionally relevant to enhanced biological phosphorus removal processes Environ. Sci. Technol. 2010, 44 (22) 8601-8608
18. Schuler, A. J.; Jenkins, D. Enhanced biological phosphorus removal from wastewater by biomass with different phosphorus contents, part I: Experimental results and comparison with metabolic models Water Environ. Res. 2003, 75 (6) 485-498
19. Majed, N.; Onnis-Hayden, A.; Christensson, M.; Welander, T.; Gu, A. Z. Phosphorous Removal Kinetics and Operational Issues with a Lab-Scale Advanced IFAS-EBPR-MBR Process In Proceedings, WEFTEC08; Chicago, IL, 2008.
20. Jenkins, D.; Richards, M. G.; Daigger, G. T. Manual on the Causes and Control of Activated Sludge Bulking and Foaming. 2 nd ed., Lewis: London, 1993.
21. Zilles, J. L.; Peccia, J.; Kim, M. W.; Hung, C. H.; Noguera, D. R. Involvement of Rhodocyclus-related organisms in phosphorus removal in full-scale wastewater treatment plants Appl. Environ. Microbiol. 2002, 68 (6) 2763-2769
22. Daims, H.; Lucker, S.; Wagner, M. daime, a novel image analysis program for microbial ecology and biofilm research Environ. Microbiol. 2006, 8 (2) 200-213
23. Liu, W. T.; Nakamura, K.; Matsuo, T.; Mino, T. Internal energy-based competition between polyphosphate- and glycogen-accumulating bacteria in biological phosphorus removal reactors-Effect of P/C feeding ratio Water Res. 1997, 31 (6) 1430-1438
24. Erdal, U. G.; Erdal, Z. K.; Daigger, G. T.; Randall, C. W. Is it PAO-GAO competition or metabolic shift in EBPR system? Evidence from an experimental study Water Sci. Technol. 2008, 58 (6) 1329-1334
25. Lopez-Vazquez, C. M.; Oehmen, A.; Hooijmans, C. M.; Brdjanovic, D.; Gijzen, H. J.; Yuan, Z. G.; van Loosdrecht, M. C. M. Modeling the PAO-GAO competition: Effects of carbon source, pH and temperature Water Res. 2009, 43 (2) 450-462
26. Kong, Y. H.; Nielsen, J. L.; Nielsen, P. H. Identity and ecophysiology of uncultured actinobacterial polyphosphate-accumulating organisms in full-scale enhanced biological phosphorus removal plants Appl. Environ. Microbiol. 2005, 71 (7) 4076-4085
27. Ault-Riche, D.; Fraley, C. D.; Tzeng, C. M.; Kornberg, A. Novel assay reveals multiple pathways regulating stress-induced accumulations of inorganic polyphosphate in Escherichia coli J. Bacteriol. 1998, 180 (7) 1841-1847
28. Bond, P. L.; Erhart, R.; Wagner, M.; Keller, J.; Blackall, L. L. Identification of some of the major groups of bacteria in efficient and nonefficient biological phosphorus removal activated sludge systems Appl. Environ. Microbiol. 1999, 65 (9) 4077-4084
29. Seviour, R.; Kragelund, C.; Kong, Y.; Eales, K.; Nielsen, J.; Nielsen, P. Ecophysiology of the Actinobacteria in activated sludge systems Antonie Van Leeuwenhoek Int. J. Gen. Mol. Microbiol. 2008, 21-33
30. Dai, Y.; Yuan, Z. G.; Wang, X. L.; Oehmen, A.; Keller, J. Anaerobic metabolism of Defluviicoccus vanus related glycogen accumulating organisms (GAOs) with acetate and propionate as carbon sources Water Res. 2007, 41 (9) 1885-1896
31. Meyer, R. L.; Saunders, A. M.; Blackall, L. L. Putative glycogen-accumulating organisms belonging to the Alphaproteobacteria identified through rRNA-based stable isotope probing Microbiol.-Sgm 2006, 152, 419-429
32. Saunders, A. M.; Oehmen, A.; Blackall, L. L.; Yuan, Z.; Keller, J. The effect of GAOs (glycogen accumulating organisms) on anaerobic carbon requirements in full-scale Australian EBPR (enhanced biological phosphorus removal) plants Water Sci. Technol. 2003, 47 (11) 37-43
33. Zhou, Y.; Pijuan, M.; Oehmen, A.; Yuan, Z. G. The source of reducing power in the anaerobic metabolism of polyphosphate accumulating organisms (PAOs)-A mini-review Water Sci. Technol. 2010, 61 (7) 1653-1662
34. Martin, H. G.; Ivanova, N.; Kunin, V.; Warnecke, F.; Barry, K. W.; McHardy, A. C.; Yeates, C.; He, S. M.; Salamov, A. A.; Szeto, E.; Dalin, E.; Putnam, N. H.; Shapiro, H. J.; Pangilinan, J. L.; Rigoutsos, I.; Kyrpides, N. C.; Blackall, L. L.; McMahon, K. D.; Hugenholtz, P. Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities Nat. Biotechnol. 2006, 24 (10) 1263-1269
35. Wilmes, P.; Andersson, A. F.; Lefsrud, M. G.; Wexler, M.; Shah, M.; Zhang, B.; Hettich, R. L.; Bond, P. L.; VerBerkmoes, N. C.; Banfield, J. F. Community proteogenomics highlights microbial strain-variant protein expression within activated sludge performing enhanced biological phosphorus removal ISME J. 2008, 2 (8) 853-864
36. Louie, T. M.; Mah, T. J.; Oldham, W.; Ramey, W. D. Use of metabolic inhibitors end gas chromatography/mass spectrometry to study poly-beta-hydroxyalkanoates metabolism involving cryptic nutrients in enhanced biological phosphorus removal systems Water Res. 2000, 34 (5) 1507-1514
37. Zhou, Y.; Pijuan, M.; Zeng, R. J.; Yuan, Z. G. Involvement of the TCA cycle in the anaerobic metabolism of polyphosphate accumulating organisms (PAOs) Water Res. 2009, 43 (5) 1330-1340
38. Erdal, U.; Erdal, Z.; Daigger, G.; Randall, C. Is it PAO-GAO competition or metabolic shift in EBPR system? Evidence from an experimental study Water Sci. Technol. 2008, 1329-1334
39. Pijuan, M.; Guisasola, A.; Baeza, J.; Carrera, J.; Casas, C.; Lafuente, J. Net P-removal deterioration in enriched PAO sludge subjected to permanent aerobic conditions J. Biotechnol. 2006, 117-126
40. Brdjanovic, D.; van Loosdrecht, M. C. M.; Hooijmans, C. M.; Mino, T.; Alaerts, G. J.; Heijnen, J. J. Bioassay for glycogen determination in biological phosphorus removal systems Water Sci. Technol. 1998, 37 (4-5) 541-547
41. Kortstee, G. J. J.; Appeldoorn, K. J.; Bonting, C. F. C.; van Niel, E. W. J.; van Veen, H. W. Recent developments in the biochemistry and ecology of enhanced biological phosphorus removal Biochemistry (Moscow) 2000, 65 (3) 332-340
42. Wexler, M.; Richardson, D. J.; Bond, P. L. Radiolabelled proteomics to determine differential functioning of Accumulibacter during the anaerobic and aerobic phases of a bioreactor operating for enhanced biological phosphorus removal Environ. Microbiol. 2009, 11 (12) 3029-3044
43. Bucci, V.; Majed, N.; Hellweger, F. L.; Gu, A. Z. Heterogeneity of intracellular polymer storage states in enhanced biological phosphorus removal (EBPR) - Observation and modeling Environ. Sci. Technol. 2012, 46 (6) 3244-3252