Bafibrinase: A non-toxic, non-hemorrhagic, direct-acting fibrinolytic serine protease from Bacillus sp. strain AS-S20-I exhibits in vivo anticoagulant activity and thrombolytic potency

Biochimie

Volumn 94, Issue 6, 2012, Pages 1300-1308

  Mukherjee, Ashis K ^a,d   Rai, Sudhir K ^a,e   Thakur, Rupamoni ^a   Chattopadhyay, Pronobesh ^b   Kar, Santosh K ^c  

^a TEZPUR UNIVERSITY   (India)

^b Defence R D Organization   (India)

^c JAWAHARLAL NEHRU UNIVERSITY   (India)

^d UNIVERSITY OF NORTHERN COLORADO   (United States)

^e EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

Anticoagulant protease; Bacillus protease; Fibrinolytic enzyme; Peptide mass fingerprinting; Plasmin like enzyme; Thrombolytic activity

Indexed keywords

BACTERIAL ENZYME; BAFIBRINASE; BENZYLSULFONYL FLUORIDE; CASEIN; EDETIC ACID; ENZYME INHIBITOR; FIBRIN; FIBRINOGEN; IODOACETAMIDE; PLASMIN; SERINE PROTEINASE; THROMBIN; UNCLASSIFIED DRUG;

ACUTE TOXICITY; ANIMAL EXPERIMENT; ANTICOAGULATION; ARTICLE; BACILLUS; BLOOD CLOT LYSIS; CARDIOVASCULAR DISEASE; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ERYTHROCYTE; HEMOLYSIS; HUMAN; HUMAN CELL; IN VIVO STUDY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOUSE; NONHUMAN; PEPTIDE ANALYSIS; PEPTIDE MASS FINGERPRINTING; PROTEIN ANALYSIS; THROMBOSIS;

ANIMALS; ANTICOAGULANTS; BACILLUS; BACTERIAL PROTEINS; FIBRIN; FIBRINOLYSIS; FIBRINOLYTIC AGENTS; HUMANS; MICE; SERINE ENDOPEPTIDASES; SERINE PROTEASES; THROMBOLYTIC THERAPY;

BACILLUS SP.; MAMMALIA;

EID: 84860466299     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2012.02.027     Document Type: Article

References (32)

1. Y. Mine, A.H. Wong, and B. Jiang Fibrinolytic enzymes in Asian traditional fermented foods Food Rev. Int. 38 2005 243 250 (Pubitemid 40118098)
2. I. Kontopaulou Oral contraceptives effects on the vascular component: thromboembolic parameters Ann. N. Y. Acad. Sci. 900 2000 228 236
3. V.J. Marder, and V. Novokhatny Direct fibrinolytic agents: biochemical attributes, preclinical foundation and clinical potential J. Thromb. Haemost. 8 2010 433 444
4. H. Mihara, H. Sumi, T. Yoneta, H. Mizumoto, R. Ikeda, M. Seiki, and M. Maruyama A novel fibrinolytic enzyme extracted from the earthworm Lumbricus rubellus Jpn. J. Physiol. 41 1991 461 472
5. L.I. Mukhametova, R.B. Aisina, G. Lomakina, and S.D. Varfolomeev Characterization of urokinase type plasminogen activator modified by phenylglyoxal Bioorg. Khim. 28 2002 308 314
6. T. Nakamura, Y. Yamagata, and E. Ichishima Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (natto) Biosci. Biotechnol. Biochem. 56 1992 1869 1871
7. J.S. Jang, D.O. Kang, M.J. Chun, and S.M. Byun Molecular cloning of a subtilisin J gene from Bacillus stearothermophilus and its expression in Bacillus subtilis Biochem. Biophys. Res. Commun. 184 1992 277 282
8. Y. Yang, L. Jiang, L. Zhu, Y. Wu, and S. Yang Thermal stable and oxidation-resistant variant of subtilisin E J. Biotechnol. 81 2000 113 118
9. A.K. Mukherjee, and S.K. Rai A statistical approach for the enhanced production of alkaline protease showing fibrinolytic activity from a newly isolated Gram-negative Bacillus sp. strain AS-S20-I New Biotech. 28 2011 182 189
10. O.H. Lowry, N.J. Rosebrough, A.L. Farr, and R.J. Randall Protein measurement with folin phenol reagent J. Biol. Chem. 193 1951 265 275
11. U.K. Laemmli Cleavage of structural proteins during assembly of the head of bacteriophage T4 Nature 227 1970 680 685
12. A. Shevchenko, A. Loboda, A. Shevchenko, W. Ens, and K.G. Standing MALDI quadrupole time-of-flight mass spectrometry: a powerful tool for proteomic research Anal. Chem. 72 2000 2132 2141 (Pubitemid 30316592)
13. S.K. Rai, and A.K. Mukherjee Statistical optimization of production, purification and industrial application of a laundry detergent and organic solvent-stable subtilisin-like serine protease (Alzwiprase) from Bacillus subtilis DM-04 Biochem. Eng. J. 48 2010 173 180
14. A.K. Mukherjee Characterization of a novel pro-coagulant metalloprotease (RVBCMP) possessing α-fibrinogenase and tissue haemorrhagic activity from venom of Daboia russelli russelli (Russell's viper): Evidence of distinct coagulant and haemorrhagic sites in RVBCMP Toxicon 51 2008 923 933
15. Z. Deng, S. Wang, Q. Li, X. Ji, L. Zhang, and M. Hong Purification and characterization of a novel fibrinolytic enzyme from the polychaete, Neanthes japonica (Iznka) Bioresource. Technol. 101 2010 1954 1960
16. G. Fotakis, and J.A. Timbrell In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride Toxicol. Lett. 160 2009 171 177 (Pubitemid 41578961)
17. H. Sumi, N. Nakajima, and C. Yatagai A unique strong fibrinolytic enzyme (Katsuwokinase) in Skipjack shiokara, a Japanese traditional fermented food Comp. Biochem. Physiol. 112B 1995 543 547
18. H.K. Kim, G.T. Kim, D.K. Kim, W.A. Chio, S.H. Park, Y.K. Jeong, and I.S. Kong Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish J. Ferment. Bioeng. 84 1997 307 312
19. M. Fujita, K. Nomura, K. Hong, Y. Ito, A. Asada, and S. Nishimuro Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan Biochem. Biophys. Res. Commun. 197 1993 1340 1346
20. W. Kim, K. Chol, Y. Kim, H. Park, J. Chol, Y. Lee, H. Oh, I. Kwon, and S. Lee Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang Appl. Environ. Microbiol. 62 1996 2482 2488 (Pubitemid 26231219)
21. S.B. Kim, W. Lee, C.I. Cheigh, E.A. Choe, S.J. Lee, Y.H. Hong, H.J. Choi, and Y.R. Pyun Purification and characterization of a fibrinolytic subtilisin-like protease of Bacillus subtilis TP-6 from an indonasian fermented soybean Tempeh J. Ind. Microbiol. Biot. 33 6 2006 436 444
22. C.T. Chang, M.H. Fan, F.C. Kuo, and H.Y. Sung Potent fibrinolytic enzyme from a mutant of Bacillus subtilis IMR-NK1 J. Agric. Food Chem. 48 8 2000 3210 3216
23. C.W. Kho, S.G. Park, S. Cho, D.H. Lee, P.K. Myung, and B.C. Park Confirmation of Vpr as a fibrinolytic enzyme present in extracellular proteins of Bacillus subtilis Protein Expr. Purif. 39 2005 1 7 (Pubitemid 39645377)
24. R. Agrebi, A. Haddar, M. Hajji, F. Frikha, L. Manni, K. Jellouli, and M. Nasri Fibrinolytic enzymes from a newly isolated marine bacterium Bacillus subtilis A26: characterization and statistical media optimization Can. J. Microbiol. 55 9 2009 1049 1061
25. Y. Peng, X.J. Yang, and Y.Z. Zhang Microbial fibrinolytic enzymes: an overview of source, production, properties, and thrombolytic activity in vivo Appl. Microbiol. Biotechnol. 67 2005 209 214
26. F.S. Markland Snake venom fibrinogenolytic and fibrinolytic enzymes: an update inventory Thromb. Haemost. 79 1998 668 674
27. Liang Xiuxia, Chen Jiashu, Zhou Yingna, Qiu Pengxin, and Yan Guangmei Purification and biochemical characterization of F IIa, a fibrinolytic enzyme from Agkistrodon acutus venom Toxicon 39 2001 1133 1139 (Pubitemid 32295880)
28. L.L. Shen, R.P. McDonagh, J. McDonagh, and J. Hermans Early events in the plasmin digestion of fibrinogen and fibrin J. Biol. Chem. 252 1977 6184 6189
29. V.J. Marder, K. Landskroner, V. Novokhatny, T.P. Zimmerman, M. Kong, J.J. Kanouse, and G. Jesmok Plasmin induces local thrombolysis without causing hemorrhage: a comparison with tissue plasminogen activator in the rabbit Thromb. Haemost. 86 2001 739 745 (Pubitemid 32834181)
30. V.J. Marder, R. Jahan, T. Gruber, A. Goyal, and V. Arora Thrombolysis with plasmin: implications for stroke treatment Stroke 41 2010 S45 S49
31. S. Sherry Recombinant tissue plasminogen activator(rt-PA): is it the thrombolytic agent of choice for an evolving acute myocardial infarction? Am. J. Cardiol. 59 1987 984 989
32. Y. Peng, Q. Huang, R.H. Zhang, and Y.Z. Zang Purification and characterization of a fibrinolytic enzyme produced by Bacillus amyloliquifaciens DC-4 screened from a traditional Chinese soybean food Comp. Biochem. Physio. Biochem. Mol. Biol. 134 2003 45 52 (Pubitemid 36078265)