Engineering GST M2-2 for high activity with indene 1,2-oxide and indication of an H-site residue sustaining catalytic promiscuity

Journal of Molecular Biology

Volumn 412, Issue 1, 2011, Pages 111-120

  Norrgård, Malena A ^a   Mannervik, Bengt ^a,b  

^a UPPSALA UNIVERSITY   (Sweden)

^b STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

active site residue; catalytic promiscuity; indene 1,2 oxide; iterative saturation mutagenesis; substrate selectivity profiles

Indexed keywords

ALANINE; AZATHIOPRINE; BENZYL ISOTHIOCYANATE; EPOXIDE; GLUTATHIONE TRANSFERASE M1; GLUTATHIONE TRANSFERASE M2; GLUTATHIONE TRANSFERASE M2 2; GLYCINE; INDENE 1,2 OXIDE; ISOLEUCINE; SERINE; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME ENGINEERING; ENZYME SPECIFICITY; HUMAN; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN TARGETING;

EID: 84860390394     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2011.07.039     Document Type: Article

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