Evaluation of four RNA extraction methods for gene expression analyses of Cryptosporidium parvum and Toxoplasma gondii oocysts

Journal of Microbiological Methods

Volumn 89, Issue 3, 2012, Pages 185-192

  See, Mary J ^a   Staggs, Sarah E ^b   Dubey, J P ^c   Villegas, Eric N ^a,b  

^a UNIVERSITY OF CINCINNATI   (United States)

^b U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Cryptosporidium; Microarray; MRNA isolation; Oocyst; Real time PCR; Toxoplasma gondii

Indexed keywords

COMPLEMENTARY DNA; GENOMIC DNA; HEAT SHOCK PROTEIN 70; MESSENGER RNA;

ANALYTICAL EQUIPMENT; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM BETA TUBULIN GENE; CRYPTOSPORIDIUM PARVUM; GENE; GENE EXPRESSION; GENE NUMBER; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OOCYST; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA DEGRADATION; RNA EXTRACTION; RNA EXTRACTION KIT; RNA ISOLATION; RNA TRANSCRIPTION; TOXOPLASMA GONDII;

ANIMALS; CELL LINE; CRYPTOSPORIDIUM PARVUM; GENE EXPRESSION PROFILING; MICE; OOCYSTS; RNA, PROTOZOAN; SPECIMEN HANDLING; TOXOPLASMA;

COCCIDIA; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; MAMMALIA; TOXOPLASMA GONDII;

EID: 84860368330     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.03.010     Document Type: Article

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