Obtaining highly purified Toxoplasma gondii oocysts by a discontinuous cesium chloride gradient

Journal of Visualized Experiments

Volumn , Issue 33, 2010, Pages

  Staggs, Sarah E ^a   See, Mary Jean ^b   Dubey, J P ^c   Villegas, Eric N ^b,d  

^a Dynamac Inc   (United States)

^b UNIVERSITY OF CINCINNATI   (United States)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

^d U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 77952581160     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1420     Document Type: Article

References (8)

1. Dubey, J.P. et al. Prevalence of viable Toxoplasma gondii in beef, chicken, and pork from retail meat stores in the United States: risk assessment to consumers. J. Parasitol. 91, 1082-93 (2005).
2. Tenter, A.M., Heckeroth, A.R. & Weiss, L.M. Toxoplasma gondii: from animals to humans. Int. J. Parasitol. 30, 1217-58 (2000).
3. Bowie, W.R. et al. Outbreak of toxoplasmosis associated with municipal drinking water. Lancet 350, 173-7 (1997).
4. de Moura, L. et al. Waterborne toxoplasmosis, Brazil, from field to gene. Emerg. Infect. Dis. 12, 326-9 (2006).
5. Jones, J.L. & Dubey, J.P. Waterborne toxoplasmosis-Recent developments. Exp. Parasitol. doi: 10.1016/j.exppara.2009.03.013 (2009).
6. Dumetre, A. & Darde, M.L. How to detect Toxoplasma gondii oocysts in environmental samples? FEMS Microbiol. Rev. 27, 651-61 (2003).
7. Dumetre, A. & Darde, M.L. Purification of Toxoplasma gondii oocysts by cesium chloride gradient. J. Microbiol. Methods 56, 427-30 (2004).
8. Dubey, J.P. Toxoplasmosis of Animals and Man. 2nd ed. CRC Press, Inc., Boca Raton, FL. In press (2009).