Extraction and Separation of Volatile and Fixed Oils from Seeds of Myristica fragrans by Supercritical CO 2: Chemical Composition and Cytotoxic Activity on Caco-2 Cancer Cells

Journal of Food Science

Volumn 77, Issue 4, 2012, Pages

  Piras, Alessandra ^a   Rosa, Antonella ^a   Marongiu, Bruno ^a   Atzeri, Angela ^a   Dessì, M Assunta ^a   Falconieri, Danilo ^a   Porcedda, Silvia ^a  

^a UNIVERSITY OF CAGLIARI   (Italy)

Author keywords

Cytotoxic activity; Essential oil; Fixed oil; Myristica fragrans; Supercritical extraction

Indexed keywords

1,3 DIOXOLANE DERIVATIVE; ANTINEOPLASTIC AGENT; BENZYL DERIVATIVE; CARBON DIOXIDE; DRUG DERIVATIVE; ESSENTIAL OIL; FATTY ACID; MYRISTICIN; PYROGALLOL; SABINENE; SOLVENT; TERPENE; VEGETABLE OIL;

ARTICLE; CELL STRAIN CACO 2; CELL SURVIVAL; CHEMISTRY; COLON TUMOR; COMPARATIVE STUDY; DISTILLATION; DRUG DEVELOPMENT; DRUG EFFECT; HEAT; HUMAN; ISOLATION AND PURIFICATION; NUTMEG; PLANT SEED; PRESSURE; SUPERCRITICAL FLUID CHROMATOGRAPHY;

ANTINEOPLASTIC AGENTS, PHYTOGENIC; BENZYL COMPOUNDS; CACO-2 CELLS; CARBON DIOXIDE; CELL SURVIVAL; CHROMATOGRAPHY, SUPERCRITICAL FLUID; COLONIC NEOPLASMS; DIOXOLANES; DISTILLATION; DRUG DISCOVERY; FATTY ACIDS; HOT TEMPERATURE; HUMANS; MONOTERPENES; MYRISTICA FRAGRANS; OILS, VOLATILE; PLANT OILS; PRESSURE; PYROGALLOL; SEEDS; SOLVENTS;

MYRISTICA FRAGRANS;

EID: 84860005206     PISSN: 00221147     EISSN: 17503841     Source Type: Journal    
DOI: 10.1111/j.1750-3841.2012.02618.x     Document Type: Article

References (19)

1. Adams RP. 2007. Identification of essential oil components by Gas Chromatography/Mass Spectroscopy, 4th ed. Carol Stream, Ill.: Allured Publishing Corporation.
2. Barceloux DG. 2008. Medical Toxicology of Natural Substances: Foods, Fungi, Medicinal Herbs, Toxic Plants, and Venomous Animals. Hoboken, N.J.: John Wiley & Sons, p 67-70.
3. Chirathaworn C, Kongcharoensuntorn W, Dechdoungchan T, Lowanitchapat A, Sanguanmoo P, Poovorawan Y. 2007. Myristica fragrans Houtt. Methanolic Extract induces Apoptosis in a Human Leukemia Cell Line through SIRT1 mRNA Downregulation. J Med Assoc Thai. 90(11):2422-8.
4. Choo Li-C, Wong S-M, Liew K-Y. 1999. Essential oil of nutmeg pericarp. J Sci Food Agric. 79:1954-7.
5. Christie WW. 1993. Preparation of ester derivatives of fatty acids for chromatographic analysis. In: Christie WW, editor. Advantage in Lipid Methodology-Two. The Oily Press: Dundee, Scotland. p 69-111.
6. Council of Europe. 1997. European Pharmacopoeia, 3rd ed., Council of Europe Press: Strasbourg. 121 p.
7. Dighe VV, Charegaonkar GA. 2009. HPTLC Analysis of Myristicin and Safrole in Seed Powder of Myristica fragrans Houtt. J Planar Chromatogr. 22(6):445-8.
8. Di Stefano V, Pitonzo R, Schillaci D. 2011 Antimicrobial and antiproliferative activity of Athamanta sicula L. (Apiaceae). Phcog Mag. 7(25):31-4.
9. Dorman HJ, Deans SG. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol. 88(2):308-16.
10. Edris AE. Pharmaceutical and Therapeutic Potentials of Essential Oils and Their Individual Volatile Constituents: a review. Phytother Res (Forthcoming) DOI:.
11. 2 extraction-comparison with steam-distilled oils-comparison of East Indian, West Indian and Papuan oils. Int J Food Sci Tech. 33(3):215-23.
12. El-Alfy AT, Wilson L, ElSohly MA, Abourashedd EA. 2009. Towards a better understanding of the psychopharmacology of nutmeg: Activities in the mouse tetrad assay. J Ethnopharmacol. 126(2):280-6.
13. 2 extraction of nutmeg oil: Experiments and modelling. J of Supercritical Fluids. 39(1):30-9.
14. Morita T, Jinno K, Kawagishi H, Arimoto Y, Suganuma H, Inakuma T, Sugiyama K. 2003. Heptoprotective effect of myristicin from nutmeg (Myristica fragrans) on lipopolysaccharide/d-galactosamine-induced liver injury. J Agric Food Chem. 51(6):1560-5.
15. Nano JN, Nobili C, Girard-Pipau F, Rampal P. 2003. Effect of fatty acids on the growth of Caco-2 cells. Prostaglandins Leukot Essent Fatty Acids. 69(4):207-15.
16. NIST/EPA/NIH Mass spectral database library (NIST 02); National Institute of Standard and Technology. 2002. Gaithersburg, Md.
17. Ram A, Lauria P, Gupta R, Sharma VN. 1996. Hypolipidaemic effect of Myristica fragrans fruit extract in rabbits. J EthnophamLacol. 55(1):49-53.
18. Rosa A, Atzeri A, Deiana M, Melis MP, Loru D, Incani A, Cabboi B, Dessì MA. 2011. Effect of aqueous and lipophilic mullet (Mugil cephalus) bottarga extracts on the growth and lipid profile of intestinal Caco-2 cells. J Agric Food Chem. 59(5):1658-66.
19. Schiller CJ, Klainz A, Mynett K, Gescher A. 1992. Assessment of viability of hepatocytes in suspension using the MTT assay. Toxicol in Vitro. 6(6):575-8.