Immunocytochemical colocalization of P16INK4a and Ki-67 predicts CIN2/3 and AIS/adenocarcinoma pilot studies

Cancer Cytopathology

Volumn 120, Issue 1, 2012, Pages 26-34

  Singh, Meenakshi ^a   Mockler, Daniel ^a   Akalin, Ali ^b   Burke, Stephanie ^a   Shroyer, A Laurie ^a   Shroyer, Kenneth R ^a  

^a STONY BROOK UNIVERSITY   (United States)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

AGC; CIN; Diagnostic accuracy; Dual immunocytochemical staining; HSIL

Indexed keywords

CYCLIN DEPENDENT KINASE INHIBITOR 2A; KI 67 ANTIGEN;

ARTICLE; CANCER GRADING; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FEMALE; GENOTYPE; HUMAN; IMMUNOCYTOCHEMISTRY; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MULTIPLEX POLYMERASE CHAIN REACTION; PILOT STUDY; PREDICTIVE VALUE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SENSITIVITY AND SPECIFICITY; UTERINE CERVIX CARCINOMA; UTERINE CERVIX CYTOLOGY; ADENOCARCINOMA; CYTODIAGNOSIS; EARLY DIAGNOSIS; EVALUATION; IMMUNOHISTOCHEMISTRY; METHODOLOGY; PAPILLOMAVIRUS INFECTION; SQUAMOUS CELL CARCINOMA; UTERINE CERVIX CARCINOMA IN SITU; UTERINE CERVIX TUMOR; VIROLOGY;

ADENOCARCINOMA; CARCINOMA, SQUAMOUS CELL; CERVICAL INTRAEPITHELIAL NEOPLASIA; CYCLIN-DEPENDENT KINASE INHIBITOR P16; CYTODIAGNOSIS; EARLY DETECTION OF CANCER; FEMALE; HUMANS; IMMUNOHISTOCHEMISTRY; KI-67 ANTIGEN; MULTIPLEX POLYMERASE CHAIN REACTION; PAPILLOMAVIRUS INFECTIONS; PILOT PROJECTS; SENSITIVITY AND SPECIFICITY; UTERINE CERVICAL NEOPLASMS;

EID: 84859802496     PISSN: None     EISSN: 19346638     Source Type: Journal    
DOI: 10.1002/cncy.20188     Document Type: Article

References (22)

1. Stoler MH, Schiffman M. Interobserver reproducibility of cervical cytologic and histologic interpretations: realistic estimates from the ASCUS-LSIL Triage Study. JAMA. 2001;285:1500-1505.
2. Schiffman M, Solomon D. Findings to date from the ASCUS-LSIL Triage Study (ALTS). Arch Pathol Lab Med. 2003;127:946-949.
3. Walker JL, Wang SS, Schiffman M, Solomon D. Predicting absolute risk of CIN3 during post-colposcopic follow-up: results from the ASCUS-LSIL Triage Study (ALTS). Am J Obstet Gynecol. 2006;195:341-348.
4. Sherman ME, Castle PE, Solomon D. Cervical cytology of atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesion (ASC-H): characteristics and histologic outcomes. Cancer. 2006;108:298-305.
5. Tringler B, Gup CJ, Singh M, et al. Evaluation of p16INK4a and pRb expression in cervical squamous and glandular neoplasia. Hum Pathol. 2004;35:689-696.
6. Klaes R, Friedrich T, Spitkovsky D, et al. Overexpression of p16(INK4A) as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri. Int J Cancer. 2001;92:276-284.
7. Bergeron C, Ordi J, Schmidt D, Trunk MJ, Keller T, Ridder R. Conjunctive p16INK4a testing significantly increases accuracy in diagnosing high-grade cervical intraepithelial neoplasia. Am J Clin Pathol. 2010;133:395-406.
8. Ordi J, Garcia S, del Pino M, et al. p16 INK4a immunostaining identifies occult CIN lesions in HPV-positive women. Int J Gynecol Pathol. 2009;28:90-97.
9. Samarawardana P, Dehn DL, Singh M, et al. p16(INK4a) is superior to high-risk human papillomavirus testing in cervical cytology for the prediction of underlying high-grade dysplasia. Cancer Cytopathol. 2010;118: 146-156.
10. Gerdes J, Schwab U, Lemke H, Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983;31:13-20.
11. Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown. J Cell Physiol. 2000;182:311-322.
12. Yu L, Wang L, Zhong J, Chen S. Diagnostic value of p16INK4A, Ki-67, and human papillomavirus L1 capsid protein immunochemical staining on cell blocks from residual liquid-based gynecologic cytology specimens. Cancer Cytopathol. 2010;118:47-55.
13. Longatto Filho A, Utagawa ML, Shirata NK, et al. Immunocytochemical expression of p16INK4A and Ki-67 in cytologically negative and equivocal pap smears positive for oncogenic human papillomavirus. Int J Gynecol Pathol. 2005;24:118-124.
14. Nishiwaki M, Yamamoto T, Tone S, et al. Genotyping of human papillomaviruses by a novel one-step typing method with multiplex PCR and clinical applications. J Clin Microbiol. 2008;46:1161-1168.
15. Klaes R, Benner A, Friedrich T, et al. p16INK4a immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. Am J Surg Pathol. 2002;26:1389-1399.
16. Negri G, Egarter-Vigl E, Kasal A, Romano F, Haitel A, Mian C. p16INK4a is a useful marker for the diagnosis of adenocarcinoma of the cervix uteri and its precursors: an immunohistochemical study with immunocytochemical correlations. Am J Surg Pathol. 2003;27:187-193.
17. Jones DL, Munger K. Interactions of the human papillomavirus E7 protein with cell cycle regulators. Semin Cancer Biol. 1996;7:327-337.
18. Khleif SN, DeGregori J, Yee CL, et al. Inhibition of cyclin D-CDK4/CDK6 activity is associated with an E2Fmediated induction of cyclin kinase inhibitor activity. Proc Natl Acad Sci U S A. 1996;93:4350-4354.
19. Riethdorf L, Riethdorf S, Lee KR, Cviko A, Loning T, Crum CP. Human papillomaviruses, expression of p16, and early endocervical glandular neoplasia. Hum Pathol. 2002;33:899-904.
20. Bose S, Evans H, Lantzy L, Scharre K, Youssef E. p16(INK4A) is a surrogate biomarker for a subset of human papilloma virus-associated dysplasias of the uterine cervix as determined on the Pap smear. Diagn Cytopathol. 2005;32:21-24.
21. Schmidt D, Bergeron C, Denton KJ, Ridder R. p16/ki-67 Dual-stain cytology in the triage of ASCUS and LSIL papanicolaou cytology: results from the european equivocal or mildly abnormal papanicolaou cytology study. Cancer Cytopathol. 2011;119:158-166.
22. Petry KU, Schmidt D, Scherbring S, et al. Triaging Pap cytology negative, HPV positive cervical cancer screening results with p16/Ki-67 Dual-stained cytology. Gynecol Oncol. 2011;121:505-509.