Purification and characterization of a novel β-1,3-1,4-glucanase (lichenase) from thermophilic Rhizomucor miehei with high specific activity and its gene sequence

Journal of Agricultural and Food Chemistry

Volumn 60, Issue 9, 2012, Pages 2354-2361

  Tang, Yanbin ^a   Yang, Shaoqing ^a   Yan, Qiaojuan ^a   Zhou, Peng ^a   Cui, Jian ^a   Jiang, Zhengqiang ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

1,3 1,4 glucanase; Characterization; Gene cloning; Purification; Rhizomucor miehei

Indexed keywords

A-CARBON; AMINO ACID SEQUENCE; BIOCHEMICAL PROPERTIES; CRUDE ENZYMES; EXTRACELLULAR; GEL FILTRATION; GENE CLONING; GENE SEQUENCES; HIGH-LEVEL PRODUCTION; PAECILOMYCES; PURIFIED ENZYME; RHIZOMUCOR MIEHEI; SPECIFIC ACTIVITY;

AMINO ACIDS; CHARACTERIZATION; CLONING; GENE ENCODING; INDUSTRIAL APPLICATIONS; ISOMERS; PURIFICATION;

ENZYME ACTIVITY;

COMPLEMENTARY DNA; GLYCOSIDASE; LICHENINASE;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; RHIZOMUCOR;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; RHIZOMUCOR; SUBSTRATE SPECIFICITY;

HORDEUM; RHIZOMUCOR MIEHEI;

EID: 84859774681     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf2049799     Document Type: Article

References (32)

1. Han, Y. J.; Chen, H. Z. Biochemical characterization of a maize stover β-exoglucanase and its use in lignocelluloses conversion. Bioresour. Technol. 2010, 101, 6111-6117.
2. Furtado, G. P.; Ribeiro, L. F.; Santos, C. R.; Tonoli, C. C.; de Souza, A. R.; Oliveira, R. R.; Murakami, M. T.; Ward, R. J. Biochemical and structural characterization of a β-1,3-1,4-glucanase from Bacillus subtilis 168. Process Biochem. 2011, 46, 1202-1206.
3. Hrmova, M.; Fincher, G. B. Structure-function relationships of β- D-glucan endo- and exohydrolases from higher plants. Plant Mol. Biol. 2001, 47, 73-91. (Pubitemid 32804498)
4. Planas, A. Bacterial β-1,3-1,4-glucanase: structure, function and protein engineering. Biochim. Biophys. Acta 2000, 1543, 361-382.
5. Tang, X. J.; Guo, Q. H.; Chen, Q. H.; Zhang, X. Y.; Ali, M. A. M. Medium optimization for the production of thermal stable β-glucanase by Bacillus subtilis ZJF A5 using response surface methodology. Bioresour. Technol. 2004, 93, 175-183.
6. Teng, D.; Wang, J. H.; Fan, Y.; Yang, Y. L.; Tian, Z. G.; Luo, J.; Yang, G. P.; Zhang, F. Cloning of β-1,3-1,4-glucanase gene from Bacillus licheniformis EGW039(CGMCC 0635) and its expression in Eschrichia coli BL21 (DE3). Appl. Microbiol. Biotechnol. 2006, 72 (4), 705-712. (Pubitemid 44454930)
7. Murray, P. G.; Grassick, A.; Laffey, C. D.; Cuffe, M. M.; Higgins, T.; Savage, A. V.; Planas, A.; Tuohy, M. G. Isolation and characterization of a thermostable endo-β-glucanase active on 1,3-1,4-β-D-glucans from the aerobic fungus Talaromyces emersonii CBS 814.70. Enzyme Microb. Technol. 2001, 29, 90-98. (Pubitemid 32566389)
8. Celestino, K. R. S.; Cunha, R. B.; Felix, C. R. Characterization of a β-glucanase produced by Rhizopus microspores var. microspores, and its potential for application in the brewing industry. BMC Biochem. 2006, 7, 23.
9. Kaur, J.; Chadha, B. S.; Kumar, B. A.; Saini, H. S. Purification and characterization of two endoglucanases from Melanocarpus sp. MTCC 3922. Bioresour. Technol. 2007, 98, 74-81. (Pubitemid 44314095)
10. Hong, M. R.; Kim, Y. S.; Joo, A. R.; Lee, J. K.; Kim, Y. S.; Oh, D. K. Purification and characterization of a thermostable β-1,3-1,4-glucanase from Laetiporus sulphureus var miniatus. J. Microbiol. Biotechnol. 2009, 19, 818-822.
11. Maheshwari, R.; Bharadwaj, G.; Bhat, M. K. Thermophilic fungi: their physiology and enzymes. Microbiol. Mol. Biol. Rev. 2000, 64, 461-488.
12. Yang, S. Q.; Yan, Q. J.; Jiang, Z. Q.; Fan, G. S.; Wang, L. Biochemical characterization of a novel thermostable β-1,3-1,4- glucanase (lichenase) from Paecilomyces thermophila. J. Agric. Food Chem. 2008, 56 (13), 5345-5351. (Pubitemid 352039145)
13. Rao, M. B.; Tanksale, A. M.; Ghatge, M. S.; Deshpande, V. V. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev. 1998, 62, 597-635. (Pubitemid 28415546)
14. Wu, X. Y.; JÄÄskelÄinen, S.; Linko, W. Y. Purification and partial characterization of Rhizomucor miehei lipase for ester synthesis. Appl. Biochem. Biotechnol. 1996, 59, 145-158. (Pubitemid 126719191)
15. Boyce, A.; Walsh, G. Production, purification and applicationrelevant characterization of an endo-β-1,3(4)-glucanase from Rhizomucor miehei. Appl. Microbiol. Biotechnol. 2007, 76, 835-841. (Pubitemid 47340938)
16. Takó, M.; Tóth, A.; Nagy, L. G.; Krisch, J.; Vágvölgyi, C.; Papp, T. A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei. Antonie van Leeuwenhoek 2010, 97, 1-10.
17. Fawzi, E. M. Highly thermostable xylanase purified from Rhizomucor miehei NRL 3169. Acta Biol. Hung. 2011, 62, 85-94.
18. Bailey, M. J.; Biely, P.; Poutanen, K. Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 1992, 23, 257-270.
19. Miller, G. L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 1959, 31, 426-428.
20. Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951, 193, 265-275.
21. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
22. Zacharius, R. M.; Zell, T. E.; Morrison, J. H.; Woodlock, J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal. Biochem. 1969, 30, 148-152.
23. Dubois, M.; Gilles, K. A.; Hamilton, J. K.; Rebe, P. A.; Smith, F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956, 28, 350-356.
24. Sambrook, J.; Russell, D. W. Molecular Clonging: A Laboratory Manual, 3rd ed.; Cold Spring Harbor Laboratory: Cold Spring Harbor, NY, 2001.
25. Rose, T. M.; Schultz, E. R.; Henikoff, J. G.; Pietrokovski, S.; McCallum, C. M.; Henikoff, S. Consensus degenerate hybrid oligonucleotide primers for amplification of distantly related sequences. Nucleic Acids Res. 1998, 26, 1628-1635. (Pubitemid 28291796)
26. Chen, H.; Li, X. L.; Ljungdahl, L. G. Sequencing of a 1,3-1,4-β- D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin. J. Bacteriol. 1997, 179, 6028-6034. (Pubitemid 27419025)
27. Görlach, J. M.; Knaap, E. V. D.; Walton, J. D. Cloning and targeted disruption of MLG1, a gene encoding two of three extracellular mixed-linked glucanases of Cochlibolus carbonum. Appl. Environ. Microbiol. 1998, 64, 385-391. (Pubitemid 28079794)
28. Grishutin, S. G.; Gusakov, A. V.; Dzedzyulya, E. I.; Sinitsyn, A. P. A lichenase-like family 12 endo-(1?4)-β-glucanase from Aspergillus japonicas: study of the substrate specificity and mode of action on β- glucans in comparison with other glycoside hydrolases. Carbohydr. Res. 2006, 341, 218-229. (Pubitemid 43042605)
29. Parry, N. J.; Beever, D. E.; Owen, E.; Nerinckx, W.; Claeyssens, M.; Beeumen, J. V.; Bhat, M. K. Biochmical characterization and mode of action of a thermostable endoglucanase purified from Thermoascus aurantiacus. Arch. Biochem. Biophys. 2002, 404, 242-253.
30. Wen, T. N.; Chen, J. L.; Lee, S. H.; Yang, N. S.; Shyur, L. F. A truncated Fibrobacter succinogenes 1,3-1,4-β-glucanase with improved enzymatic activity and thermotolerance. Biochemistry 2005, 44, 9197-9205.
31. Apiraksakorn, J.; Nitisinprasert, T.; Levin, R. E. Grass degrading β-1,3-1,4-D-glucanases from Bacillus subtilis GN156: purification and characterization of glucanase J1 and pJ2 possessing extremely acidic pI. Appl. Biochem. Biotechnol. 2008, 149, 53-66.
32. Teng, D.; Fan, Y.; Yang, Y. L.; Tian, Z. G.; Luo, J.; Wang, J. H. Codon optimization of Bacillus licheniformis β-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Appl. Microbiol. Biotechnol. 2007, 74, 705-712.