Longitudinal study comparing the dynamics of Clostridium difficile in conventional and antimicrobial free pigs at farm and slaughter

Veterinary Microbiology

Volumn 157, Issue 1-2, 2012, Pages 172-178

  Susick, E K ^a   Putnam, M ^a   Bermudez, D M ^a   Thakur, S ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

Antimicrobial resistance; Clostridium difficile; Farm; Slaughter; Swine; Toxinotyping; Toxins

Indexed keywords

AMPICILLIN; BACTERIAL TOXIN; BINARY TOXIN; CIPROFLOXACIN; ERYTHROMYCIN; METRONIDAZOLE; TETRACYCLINE; TOXIN A; TOXIN B; UNCLASSIFIED DRUG; VANCOMYCIN;

ANTIBIOTIC SENSITIVITY; ANTIMICROBIAL FREE; ARTICLE; BACTERIUM ISOLATION; CLOSTRIDIUM DIFFICILE; COMPARATIVE STUDY; DISEASE TRANSMISSION; ENVIRONMENT; FECES CULTURE; GENE DELETION; GENOTYPE PHENOTYPE CORRELATION; LONGITUDINAL STUDY; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR DYNAMICS; NONHUMAN; PATHOGENICITY; PIG FARMING; POLYMERASE CHAIN REACTION; SLAUGHTERHOUSE; TOXIN ANALYSIS;

ANIMAL HUSBANDRY; ANIMALS; ANTI-BACTERIAL AGENTS; CLOSTRIDIUM DIFFICILE; DRUG RESISTANCE, MULTIPLE, BACTERIAL; LONGITUDINAL STUDIES; MICROBIAL SENSITIVITY TESTS; PREVALENCE; SUS SCROFA; SWINE; SWINE DISEASES;

CLOSTRIDIUM DIFFICILE; SUIDAE;

EID: 84859716107     PISSN: 03781135     EISSN: 18732542     Source Type: Journal    
DOI: 10.1016/j.vetmic.2011.12.017     Document Type: Article

References (32)

1. Alvarez-Perez S., Blanco J., Bouza E., Alba P., Gibert X., Maldonado J., Garcia M. Prevalence of Clostridium difficile in diarrhoeic and non-diarrhoeic piglets. Vet. Microbiol. 2009, 137:302-305.
2. Baker A., Davis E., Rehberger T., Rosener D. Prevalence and diversity of toxigenic Clostridium perfringens and Clostridium difficile among swine herds in the Midwest. Appl. Environ. Microbiol. 2010, 76:2961-2967.
3. Baverud V., Gustafsson A., Franklin A., Aspan A., Gunnarsson A. Clostridium difficile: prevalence in horses and environment, and antimicrobial susceptibility. Equine Vet. J. 2003, 35:465-471.
4. Bergogne-Berezin E. Treatment and prevention of antibiotic associated diarrhea. Int. J. Antimicrob. Agents 2000, 16:521-526.
5. CDC Surveillance for community-associated Clostridium difficile - Connecticut, 2006. MMWR Morb. Mortal. Wkly. Rep. 2008, 57:340-343.
6. CLSI, 2007. Methods for antimicrobial susceptibility testing of anaerobic bacteria: approved standards. CLSI document M11-A7. Clinical and Laboratory Standards Institute, Wayne, PA, 32 pp.
7. Debast S., van Leengoed L., Goorhuis A., Harmanus C., Kuijper E., Bergwerff A. Clostridium difficile PCR ribotype 078 toxinotype V found in diarrhoeal pigs identical to isolates from affected humans. Environ. Microbiol. 2009, 11:505-511.
8. Debure A., Rambaud J.C., Ducluzeau R., Yurdusev N., Raibaud P. Translocation of strictly anaerobic-bacteria from the intestinal-tract to the mesenteric lymph-nodes in gnotobiotic rodents. Ann. Inst. Pasteur MIC 1987, 138:213-221.
9. Gerding D. Clindamycin, cephalosporins, fluoroquinolones, and Clostridium difficile-associated diarrhea: this is an antimicrobial resistance problem. Clin. Infect. Dis. 2004, 38:646-648.
10. Gonçalves C., Decré D., Barbut F., Burghoffer B., Petit J.C. Prevalence and characterization of a binary toxin (actin-specific ADP-ribosyltransferase) from Clostridium difficile. J. Clin. Microbiol. 2004, 42:1933-1939.
11. Hopman N.E., Keessen E.C., Harmanus C., Sanders I.M., van Leengoed L.A., Kuijper E.J., Lipman L.J. Acquisition of Clostridium difficile by piglets. Vet. Microbiol. 2011, 149:186-192.
12. Huang H.H., Wu S., Wang M.G., Zhang Y.Y., Fang H., Palmgren A.C., Weintraub A., Nord C.E. Clostridium difficile infections in a Shanghai hospital: antimicrobial resistance, toxin profile and ribotypes. Int. J. Antimicrob. Agents 2009, 33:339-342.
13. Jhung M., Thompson A., Killgore G., Zukowski W., Songer G., Warny M., Johnson S., Gerding D., McDonald L., Limbago B. Toxinotype V Clostridium difficile in humans and food animals. Emerg. Infect. Dis. 2008, 14:1039-1045.
14. Keel K., Brazier J., Post K., Weese S., Songer J. Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. J. Clin. Microbiol. 2007, 45:1963-1964.
15. Keessen E.C., van den Berkt A.J., Haasjes N.H., Hermanus C., Kuijper E.J., Lipman L.J. The relation between farm specific factors and prevalence of Clostridium difficile in slaughter pigs. Vet. Microbiol. 2011, 154:130-134.
16. Kyne L., Warny M., Qamar A., Kelly C. Association between antibody response to toxin A and protection against recurrent Clostridium difficile diarrhoea. Lancet 2001, 357:189-193.
17. Lemee L., Dhalluin A., Testelin S., Mattrat M., Maillard K., Lemeland J., Pons J. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin a), and tcdB (Toxin b) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 2004, 42:5710-5714.
18. McDonald L., Owings M., Jernigan D. Clostridium difficile infection in patients discharged from US short-stay hospitals, 1996-2003. Emerg. Infect. Dis. 2006, 12:409-415.
19. Mutlu E., Wroe A., Sanchez-Hurtado K., Brazier J., Poxton I. Molecular characterization and antimicrobial susceptibility patterns of Clostridium difficile strains isolated from hospitals in south-east Scotland. J. Med. Microbiol. 2007, 56:921-929.
20. Nagy J., Bilkei G. Neonatal piglet losses associated with Escherichia coli and Clostridium difficile infection in a Slovakian outdoor production unit. Vet. J. 2003, 166:98-100.
21. Noren T., Akerlund T., Back E., Sjober L., Persson I., Alriksson I., Burman L. Molecular epidemiology of hospital-associated and community-acquired Clostridium difficile infection in a Swedish county. J. Clin. Microbiol. 2004, 42:3635-3643.
22. Norman K.N., Harvey R.B., Scott H.M., Hume M.E., Andrews K., Brawley A.D. Varied prevalence of Clostridium difficile in an integrated swine operation. Anaerobe 2009, 15:256-260.
23. Rodriguez-Palacios A., Stämpfli H.R., Duffield T., Peregrine A.S., Trotz-Williams L.A., Arroyo L.G., Brazier J.S., Weese J.S. Clostridium difficile PCR ribotypes in calves, Canada. Emerg. Infect. Dis. 2006, 12:1730-1736.
24. Rupnik M., Avesani V., Janc M., von Eichel-Streiber C., Delmée M. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 1998, 36:2240-2247.
25. Songer J.G., Anderson M.A. Clostridium difficile: an important pathogen of food animals. Anaerobe 2006, 12:1-4.
26. Spigaglia P., Mastrantonio P. Molecular analysis of the pathogenicity locus and polymorphism in the putative negative regulator of toxin production (TcdC) among Clostridium difficile clinical isolates. J. Clin. Microbiol. 2002, 40:3470-3475.
27. Stubbs S., Rupnik M., Gibert M., Brazier J., Duerden B., Popoff M. Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol. Lett. 2000, 186:307-312.
28. Tang Y.J., Gumerlock P.H., Weiss J.B., Silva J. Specific detection of Clostridium difficile toxin A gene sequences in clinical isolates. Mol. Cell. Probes 1994, 8:463-467.
29. Thakur S., Putnam M., Fry P.R., Abley M., Gebreyes W.A. Prevalence of antimicrobial resistance and association with toxin genes in Clostridium difficile in commercial swine. Am. J. Vet. Res. 2010, 71:1189-1194.
30. Weese J., Wakeford T., Reid-Smith R., Rousseau J., Friendship R. Longitudinal investigation of Clostridium difficile shedding in piglets. Anaerobe 2010, 16:501-504.
31. Yaeger M., Funk N., Hoffman L. A survey of agents associated with neonatal diarrhea in Iowa swine including Clostridium difficile and porcine reproductive and respiratory syndrome virus. J. Vet. Diagn. Invest. 2002, 14:281-287.
32. Zhang Q., Sahin O., McDermott P., Payot S. Fitness of antimicrobial-resistant Campylobacter and Salmonella. Microbes Infect. 2006, 8:1972-1978.