Accuracy and sensitivity of residual DNA detection by QPCR is not predicted by target copy number

Biotechnology Progress

Volumn 28, Issue 2, 2012, Pages 428-434

  Verardo, Megan L ^a   Carvalho, Juliane G ^a   Delgado, Dora N ^a   Kuhns, Scott T ^a  

^a AMGEN INC   (United States)

Author keywords

DNA impurities; Genomic DNA copy number; Host cell DNA clearance; QPCR; Residual DNA

Indexed keywords

ACCURATE MEASUREMENT; CHINESE HAMSTER OVARY; COPY NUMBER; DNA CLEARANCE; DNA DETECTION; HOST CELLS; IN-PROCESS; MAMMALIAN CELL CULTURE; MANUFACTURING IS; PROCESS-RELATED IMPURITIES; PRODUCT PURIFICATION; PRODUCTION CELLS; QPCR; QUANTITATIVE POLYMERASE CHAIN REACTION; SENSITIVE ASSAY;

ASSAYS; CELL CULTURE; CELLS; GENES; GENETIC ENGINEERING; POLYMERASE CHAIN REACTION; PURIFICATION; REMOVAL;

DNA;

DNA;

ANIMAL; ARTICLE; CHO CELL; CONFORMATION; EVALUATION; GENE DOSAGE; GENETICS; HAMSTER; METHODOLOGY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; BASE SEQUENCE; CHO CELLS; CRICETINAE; DNA; GENE DOSAGE; NUCLEIC ACID CONFORMATION; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

CRICETULUS GRISEUS; MAMMALIA;

EID: 84859628245     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.731     Document Type: Article

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