Serious overestimation in quantitative pcr by circular (supercoiled) plasmid standard: Microalgal pcnaas the model gene

PLoS ONE

Volumn 5, Issue 3, 2010, Pages

  Hou, Yubo ^a,b   Zhang, Huan ^b   Miranda, Lilibeth ^a,c   Lin, Senjie ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

^b WADSWORTH CENTER   (United States)

^c TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CIRCULAR DNA; COMPLEMENTARY DNA; CYCLINE;

AMPLICON; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; DIATOM; DNA SUPERCOILING; DNA TEMPLATE; MICROALGA; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; QUANTITATIVE ANALYSIS; THALASSIOSIRA PSEUDONANA; GENE LIBRARY; GENETIC PROCEDURES; GENETICS; GENOME; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; STANDARD;

BACILLARIOPHYTA; EUKARYOTA; THALASSIOSIRA PSEUDONANA;

CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE LIBRARY; GENETIC TECHNIQUES; GENOME; MICROALGAE; PLASMIDS; POLYMERASE CHAIN REACTION; PROLIFERATING CELL NUCLEAR ANTIGEN;

EID: 77949667195     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0009545     Document Type: Article

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