Determination of pig sex in meat and meat products using multiplex real time-PCR

Meat Science

Volumn 91, Issue 3, 2012, Pages 272-276

  Abdulmawjood, A ^a   Krischek, C ^a   Wicke, M ^b   Klein, G ^a  

^a UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

AMEL; Boar taint; Pig; Real time PCR; Sexing

Indexed keywords

AMEL; BOAR TAINT; PIG; REAL TIME-PCR; SEXING;

GENES; MAMMALS; POLYMERASE CHAIN REACTION;

MEATS;

ANIMALIA; CAPRA HIRCUS; OVIS ARIES; SUIDAE;

AMELOGENIN; DNA;

ANIMAL; ARTICLE; BREEDING; CATERING SERVICE; EUROPE; FEMALE; GENETICS; HUMAN; MALE; MEAT; METHODOLOGY; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SEX DETERMINATION; SEXUAL DEVELOPMENT; STANDARD; SWINE; VALIDATION STUDY;

AMELOGENIN; ANIMALS; BREEDING; DNA; EUROPE; FEMALE; FOOD SUPPLY; HUMANS; MALE; MEAT; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SEX CHARACTERISTICS; SEX DETERMINATION ANALYSIS; SWINE;

EID: 84859563807     PISSN: 03091740     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.meatsci.2012.02.001     Document Type: Article

References (17)

1. Adams P.S. Data analysis and reporting. Bios. Advanced methods, Real time-PCR 2006, 39-61. Taylor and Francis Group. T.M. Dorak (Ed.).
2. Anonymous, regulation (EC) no. 854/2004 of the european parliament and of the council, Chapter V: Decisions concerning meat, 1(p), p. L 226/100.
3. Anonymous, Council Directive 2008/120/EC Annex I, Chapter I General Conditions, 8, p. L 47/10.
4. Bonneau M., Lebret B. Production systems and influence on eating quality of pork. Meat Science 2010, 84:293-300.
5. Bustin S., Beaulieu J.F., Huggett J., Jaggi R., Kibenge F., Olsvik P., et al. MIQE precis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments. BMC Molecular Biology 2010, 11:74.
6. Bustin S.A., Benes V., Garson J.A., Hellemans J., Huggett J., Kubista M., et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 2009, 55:611-622.
7. Claus R., Weiler U., Herzog A. Physiological aspects of androstenone and skatole formation in the boar - Review with experimental data. Meat Science 1994, 38:289-305.
8. Fontanesi L., Scotti E., Russo V. Differences of the porcine amelogenin X and Y chromosome genes (AMELX and AMELY) and their application for sex determination in pigs. Molecular Reproduction and Development 2008, 75:1662-1668.
9. Fuchs T., Nathues H., Koehrmann A., Andrews S., Brock F., Sudhaus N., et al. A comparison of the carcase characteristics of pigs immunized with a 'gonadotrophin-releasing factor (GnRF)' vaccine against boar taint with physically castrated pigs. Meat Science 2009, 83:702-705.
10. Hasegawa T., Sato F., Ishida N., Fukushima Y., Mukoyama H. Sex determination by simultaneous amplification of equine SRY and amelogenin genes. The Journal of Veterinary Medical Science 2000, 62:1109-1110.
11. Langen M., Peters U., Koerner U., Gissel C., Stanislawski D., Klein G. Semiquantitative detection of male pork tissue in meat and meat products by PCR. Meat Science 2010, 86:821-824.
12. Pfeiffer I., Brenig B. X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis aries) and European red deer (Cervus elaphus). BMC Genetics 2005, 6:16.
13. Phua A.C.Y., Abdullah R.B., Mohamed Z. A PCR-Based Sex Determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X Genes. The Journal of Reproduction and Development 2003, 49:307-311.
14. Rattanasuk S., Parnpai R., Ketudat-Cairns M. Multiplex polymerase chain reaction used for bovine embryo sex determination. The Journal of Reproduction and Development 2011, 57:539-542.
15. Schön S., Wilke T., Erdmann R. Molecular biological sex determination of processed porcine in fresh meat and meat preparations. Fleischwirtschaft 2009, 89:48-48.
16. Wang X., Seed B. High-throughput primer and probe design. Bios. Advanced methods, Real time-PCR 2006, 93-105. Taylor and Francis Group. T.M. Dorak (Ed.).
17. Weikard R., Pitra C., Kuhn C. Amelogenin cross-amplification in the family Bovidae and its application for sex determination. Molecular Reproduction and Development 2006, 73:1333-1337.