High-throughput primer and probe design

Real-time PCR

Volumn , Issue , 2007, Pages 93-106

  Wang, Xiaowei ^a   Seed, Brian ^b  

^a APPLIED BIOSYSTEMS   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85077276043     PISSN: None     EISSN: None     Source Type: Book    
DOI: None     Document Type: Chapter

References (12)

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4. Huang MM, Arnheim N, Goodman MF (1992) Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR. Nucleic Acids Res 20: 4567-4573.
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9. von Ahsen N, Wittwer CT, Schutz E (2001) Oligonucleotide melting temperatures under PCR conditions: nearest-neighbor corrections for Mg(2+), deoxynucleotide triphosphate, and dimethyl sulfoxide concentrations with comparison to alternative empirical formulas. Clin Chem 47: 1956-1961.
10. Wang X, Seed B (2003a) A PCR primer bank for quantitative gene expression analysis. Nucleic Acids Res 31: e154.
11. Wang X, Seed B (2003b) Selection of oligonucleotide probes for protein coding sequences. Bioinformatics 19: 796-802.
12. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31: 3406-3415.