Pannexin 1, an ATP release channel, is activated by caspase cleavage of its pore-associated C-terminal autoinhibitory region

Journal of Biological Chemistry

Volumn 287, Issue 14, 2012, Pages 11303-11311

  Sandilos, Joanna K ^a   Chiu, Yu Hsin ^a   Chekeni, Faraaz B ^a   Armstrong, Allison J ^a   Walk, Scott F ^a   Ravichandran, Kodi S ^a,b   Bayliss, Douglas A ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOTIC; APOPTOTIC CELLS; C TERMINUS; C-TERMINAL REGIONS; C-TERMINAL TAIL; CASPASES; CHANNEL ACTIVATION; CHANNEL ACTIVITY; CHANNEL PORE; CHANNEL REGULATION; CLEAVAGE SITES; CONSTITUTIVELY ACTIVES; PARTIAL ACTIVATION; POINT MUTATIONS; PORE BLOCKER; SMALL REGION;

AMINO ACIDS; CELL MEMBRANES;

CELL DEATH;

CASPASE; MEMBRANE PROTEIN; PANNEXIN 1; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE PERMEABILITY; CELL STRAIN HEK293; CHANNEL GATING; CONTROLLED STUDY; CROSS LINKING; CYSTEINE CROSS LINKING; ENZYME CLEAVAGE; ENZYME METABOLISM; HUMAN; IN VITRO STUDY; MEMBRANE CHANNEL; MOUSE; NONHUMAN; PATCH CLAMP; POINT MUTATION; PRIORITY JOURNAL; PROTEIN ACTIVATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN METABOLISM; PROTEIN SYNTHESIS REGULATION;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CASPASES; CELL MEMBRANE; CONNEXINS; HEK293 CELLS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; NERVE TISSUE PROTEINS; POROSITY; PROTEOLYSIS;

EID: 84859514212     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.323378     Document Type: Article

References (30)

1. MacVicar, B. A., and Thompson, R. J. (2010) Non-junction functions of pannexin 1 channels. Trends Neurosci. 33, 93-102
2. D'hondt, C., Ponsaerts, R., De Smedt, H., Bultynck, G., and Himpens, B. (2009) Pannexins, distant relatives of the connexin family with specific cellular functions? Bioessays. 31, 953-974
3. Sosinsky, G. E., Boassa, D., Dermietzel, R., Duffy, H. S., Laird, D. W., MacVicar, B., Naus, C. C., Penuela, S., Scemes, E., Spray, D. C., Thompson, R. J., Zhao, H. B., and Dahl, G. (2011) Pannexin channels are not gap junction hemichannels. Channels 5, 193-197
4. Dubyak, G. R. (2009) Both sides now: multiple interactions of ATP with pannexin 1 hemichannels. Focus on "A permeant regulating its permeation pore: inhibition of pannexin 1 channels by ATP". Am. J. Physiol. CellPhysiol. 296, C235-C241
5. Chekeni, F. B., Elliott, M. R., Sandilos, J. K., Walk, S. F., Kinchen, J. M., Lazarowski, E. R., Armstrong, A. J., Penuela, S., Laird, D. W., Salvesen, G. S., Isakson, B. E., Bayliss, D. A., and Ravichandran, K. S. (2010) Pannexin 1 channels mediate "find-me" signal release and membrane permeability during apoptosis. Nature 467, 863-867
6. Elliott, M. R., Chekeni, F. B., Trampont, P. C., Lazarowski, E. R., Kadl, A., Walk, S. F., Park, D., Woodson, R. I., Ostankovich, M., Sharma, P., Lysiak, J. J., Harden, T. K., Leitinger, N., and Ravichandran, K. S. (2009) Nucleotides released by apoptotic cells act as a find-me signal to promote phagocytic clearance. Nature 461, 282-286
7. Bao, L., Locovei, S., and Dahl, G. (2004) Pannexin membrane channels are mechanosensitive conduits for ATP. FEBS Lett. 572, 65-68 (Pubitemid 39092514)
8. Silverman, W. R., de Rivero Vaccari, J. P., Locovei, S., Qiu, F., Carlsson, S. K., Scemes, E., Keane, R. W., and Dahl, G. (2009) The pannexin 1 channel activates the inflammasome in neurons and astrocytes. J. Biol. Chem. 284, 18143-18151
9. Qiu, F., and Dahl, G. (2009) A permeant regulating its permeation pore: inhibition of pannexin 1 channels by ATP. Am. J. Physiol. Cell Physiol. 296, C250-C255
10. Billaud, M., Lohman, A. W., Straub, A. C., Looft-Wilson, R., Johnstone, S. R., Araj, C. A., Best, A. K., Chekeni, F. B., Ravichandran, K. S., Penuela, S., Laird, D. W., and Isakson, B. E. (2011) Pannexin 1 regulates α1-adrenergic receptor-mediated vasoconstriction. Circ. Res. 109, 80-85
11. Qu, Y., Misaghi, S., Newton, K., Gilmour, L. L., Louie, S., Cupp, J. E., Dubyak, G. R., Hackos, D., and Dixit, V. M. (2011) Pannexin 1 is required for ATP release during apoptosis but not for inflammasome activation. J. Immunol. 186, 6553-6561
12. Denault, J. B., and Salvesen, G. S. (2008) Apoptotic caspase activation and activity. Methods Mol. Biol. 414, 191-220
13. Williams, D. J., Puhl, H. L., 3rd, and Ikeda, S. R. (2009) Rapid modification of proteins using a rapamycin-inducible tobacco etch virus protease system. PLoS One. 4, e7474
14. Su, W., Yeku, O., Olepu, S., Genna, A., Park, J. S., Ren, H., Du, G., Gelb, M. H., Morris, A. J., and Frohman, M. A. (2009) 5-Fluoro-2-indolyl deschlorohalopemide (FIPI), a phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis. Mol. Pharmacol. 75, 437-446
15. q family subunits, phospholipase C, and a readily diffusible messenger. J. Biol. Chem. 276, 16720-16730
16. Bunse, S., Schmidt, M., Prochnow, N., Zoidl, G., and Dermietzel, R. (2010) Intracellular cysteine 346 is essentially involved in regulating Panx1 channel activity. J. Biol. Chem. 285, 38444-38452
17. Wang, J., and Dahl, G. (2010) SCAM analysis of Panx1 suggests a peculiar pore structure. J. Gen. Physiol. 136, 515-527
18. Hoshi, T., Zagotta, W. N., and Aldrich, R. W. (1990) Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science 250, 533-538 (Pubitemid 120031778)
19. Armstrong, C. M., Bezanilla, F., and Rojas, E. (1973) Destruction of sodium conductance inactivation in squid axons perfused with pronase. J. Gen. Physiol. 62, 375-391
20. Maeda, S., Nakagawa, S., Suga, M., Yamashita, E., Oshima, A., Fujiyoshi, Y., and Tsukihara, T. (2009) Structure of the connexin 26 gap junction channel at 3.5 Å resolution. Nature 458, 597-602
21. Bargiello, T. A., Tang, Q., Oh, S., and Kwon, T. (2011) Voltage-dependent conformational changes in connexin channels. Biochim. Biophys. Acta, in press
22. Morley, G. E., Taffet, S. M., and Delmar, M. (1996) Intramolecular interactions mediate pH regulation of connexin43 channels. Biophys. J. 70, 1294-1302 (Pubitemid 26073042)
23. Moreno, A. P., Chanson, M., Elenes, S., Anumonwo, J., Scerri, I., Gu, H., Taffet, S. M., and Delmar, M. (2002) Role of the carboxyl terminal of connexin43 in transjunctional fast voltage gating. Circ. Res. 90, 450-457 (Pubitemid 34633915)
24. Ek-Vitorín, J. F., Calero, G., Morley, G. E., Coombs, W., Taffet, S. M., and Delmar, M. (1996) PH regulation of connexin43: molecular analysis of the gating particle. Biophys. J. 71, 1273-1284 (Pubitemid 26299767)
25. Zagotta, W. N., Hoshi, T., and Aldrich, R. W. (1990) Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB. Science 250, 568-571 (Pubitemid 120031789)
26. Li, S., Bjelobaba, I., Yan, Z., Kucka, M., Tomic, M., and Stojilkovic, S. S. (2011) Expression and roles of pannexins in ATP release in the pituitary gland. Endocrinology 152, 2342-2352
27. Seminario-Vidal, L., Okada, S. F., Sesma, J. I., Kreda, S. M., van Heusden, C. A., Zhu, Y., Jones, L. C., O'Neal, W. K., Penuela, S., Laird, D. W., Boucher, R. C., and Lazarowski, E. R. (2011) Rho signaling regulates pannexin 1-mediated ATP release from airway epithelia. J. Biol. Chem. 286, 26277-26286
28. Ma, W., Hui, H., Pelegrin, P., and Surprenant, A. (2009) Pharmacological characterization of pannexin 1 currents expressed in mammalian cells. J. Pharmacol. Exp. Ther. 328, 409-418
29. Chekeni, F. B., and Ravichandran, K. S. (2011) The role of nucleotides in apoptotic cell clearance: implications for disease pathogenesis. J. Mol. Med. 89, 13-22
30. Lai, C. P., Bechberger, J. F., Thompson, R. J., MacVicar, B. A., Bruzzone, R., and Naus, C. C. (2007) Tumor-suppressive effects of pannexin 1 in C6 glioma cells. Cancer Res. 67, 1545-1554 (Pubitemid 46383378)