From in silico to in vitro: Modelling and production of Trichoderma reesei endoglucanase 1 and its mutant in Pichia pastoris

Journal of Biotechnology

Volumn 159, Issue 1-2, 2012, Pages 61-68

  Bayram Akcapinar, Gunseli ^a   Gul, Ozgur ^a   Sezerman, Ugur O ^a  

^a SABANCI UNIVERSITY   (Turkey)

Author keywords

Cellulase; Endoglucanase; Molecular modelling; Pichia pastoris; Site directed mutagenesis; Trichoderma reesei

Indexed keywords

CELLULASE; ENDOGLUCANASES; PICHIA PASTORIS; SITE DIRECTED MUTAGENESIS; TRICHODERMA REESEI;

AMINO ACIDS; ENZYMES; MOLECULAR DYNAMICS; MOLECULAR MODELING; MUTAGENESIS;

YEAST;

CARBOXYMETHYLCELLULOSE; CELLULASE; GLUCAN SYNTHASE; GLYCINE; LYSINE; MUCIN 4; TRICHODERMA REESEI ENDOGLUCANASE 1; UNCLASSIFIED DRUG;

ARTICLE; COMPUTER MODEL; CONTROLLED STUDY; CROSS LINKING; DRUG MANUFACTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME SUBSTRATE; HYPOCREA JECORINA; IN VITRO STUDY; MOLECULAR DYNAMICS; MOLECULAR MECHANICS; MUTANT; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIMULATION; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

BIOREACTORS; CARBOXYMETHYLCELLULOSE SODIUM; CELLULASE; COMPUTER SIMULATION; ENZYME STABILITY; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; MUTATION; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TEMPERATURE; TRICHODERMA;

HYPOCREA JECORINA; PICHIA PASTORIS;

EID: 84859511736     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.01.001     Document Type: Article

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