Evaluation of human interferon adsorption performance of Cibacron Blue F3GA attached cryogels and interferon purification by using FPLC system

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 893-894, Issue , 2012, Pages 69-76

  Doĝan, Ali ^a   Özkara, Serpil ^a   Sari, Müfrettin Murat ^b,c   Uzun, Lokman ^b   Denizli, Adil ^b  

^a INONU UNIVERSITY   (Turkey)

^b HACETTEPE UNIVERSITY   (Turkey)

^c TURKISH MILITARY ACADEMY   (Turkey)

Author keywords

Affinity purification; Cibacron Blue F3GA; Fast protein liquid chromatography; Interferon; Poly(HEMA) cryogel

Indexed keywords

2-HYDROXYETHYL METHACRYLATE; ADSORPTION CAPACITIES; ADSORPTION PERFORMANCE; ADSORPTION-DESORPTION CYCLES; AFFINITY PURIFICATION; ANTIVIRAL ACTIVITIES; CIBACRON BLUE F-3GA; CRYOGELS; DYE-LIGANDS; EFFECTING FACTORS; FAST PROTEIN LIQUID CHROMATOGRAPHY; FTIR; HUMAN GINGIVAL FIBROBLASTS; INTERACTION TIME; POLY(2-HYDROXYETHYL METHACRYLATE); SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SUPERMACROPOROUS CRYOGELS; SWELLING TEST; THEORETICAL PLATE NUMBERS; TOTAL PROTEIN;

ADSORPTION; ELECTROPHORESIS; GLYCOPROTEINS; IONIC STRENGTH; LIQUID CHROMATOGRAPHY; PHEMA; PURIFICATION; SCANNING ELECTRON MICROSCOPY; SODIUM; SODIUM SULFATE;

ANTIBIOTICS;

2 HYDROXYETHYL METHACRYLATE; CIBACRON BLUE F3GA; RECOMBINANT ALPHA INTERFERON;

ADSORPTION; ANTIVIRAL ACTIVITY; ARTICLE; CRYOGEL; DESORPTION; FAST PROTEIN LIQUID CHROMATOGRAPHY; FIBROBLAST; GINGIVA; HUMAN; HUMAN CELL; IN VITRO STUDY; INFRARED SPECTROSCOPY; IONIC STRENGTH; PH; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY;

ADSORPTION; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRYOGELS; HUMANS; HYDROGEN-ION CONCENTRATION; INTERFERON-ALPHA; METHACRYLATES; OSMOLAR CONCENTRATION; RECOMBINANT PROTEINS; TRIAZINES;

EID: 84859442417     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2012.02.036     Document Type: Article

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