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Volumn 109, Issue 2, 2012, Pages 363-371

Estimating transgene copy number in precocious trifoliate orange by TaqMan real-time PCR

Author keywords

Copy number; TaqMan real time PCR; Transgenic citrus

Indexed keywords

COPY NUMBER; ENDOGENOUS GENES; EXOGENOUS GENES; EXPRESSION LEVELS; R VALUE; REAL-TIME PCR; REAL-TIME PCR ASSAY; SOUTHERN BLOT; SOUTHERN BLOT ANALYSIS; STRONG CORRELATION; TRANSGENE; TRANSGENE COPY NUMBERS; TRANSGENIC LINES; TRANSGENIC PLANTS; TRANSGENICS;

EID: 84859433855     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-011-0101-x     Document Type: Article
Times cited : (26)

References (35)
  • 3
    • 8744279655 scopus 로고    scopus 로고
    • Two fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR
    • Bubner B, Gase K, Baldwin IT (2004) Two fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR. BMC Biotechnol 4: 14.
    • (2004) BMC Biotechnol , vol.4 , pp. 14
    • Bubner, B.1    Gase, K.2    Baldwin, I.T.3
  • 5
    • 0034102331 scopus 로고    scopus 로고
    • A broad exploration of a transgenic population of citrus: stability of gene expression and phenptype
    • Cervera M, Pina JA, Juárez J, Navarro L, Peňa L (2000) A broad exploration of a transgenic population of citrus: stability of gene expression and phenptype. Theor Appl Genet 100: 670-677.
    • (2000) Theor Appl Genet , vol.100 , pp. 670-677
    • Cervera, M.1    Pina, J.A.2    Juárez, J.3    Navarro, L.4    Peňa, L.5
  • 6
    • 4544330177 scopus 로고    scopus 로고
    • An efficient protocol for genomic DNA extraction from Citrus species
    • Cheng YJ, Guo WW, Yi HL, Pang XM, Deng XX (2003) An efficient protocol for genomic DNA extraction from Citrus species. Plant Mol Biol Rep 21: 177-178.
    • (2003) Plant Mol Biol Rep , vol.21 , pp. 177-178
    • Cheng, Y.J.1    Guo, W.W.2    Yi, H.L.3    Pang, X.M.4    Deng, X.X.5
  • 8
    • 77954554456 scopus 로고    scopus 로고
    • Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation
    • Faize M, Faize L, Burgos L (2010) Using quantitative real-time PCR to detect chimeras in transgenic tobacco and apricot and to monitor their dissociation. BMC Biotechnol 10: 53-61.
    • (2010) BMC Biotechnol , vol.10 , pp. 53-61
    • Faize, M.1    Faize, L.2    Burgos, L.3
  • 9
    • 80053930954 scopus 로고    scopus 로고
    • T-DNA direct repeat and 35S promoter methylation affect transgene expression but do not cause silencing in transgenic sweet orange
    • Fan J, Liu X, Xu SX, Xu Q, Guo WW (2011) T-DNA direct repeat and 35S promoter methylation affect transgene expression but do not cause silencing in transgenic sweet orange. Plant Cell Tissue Organ Cult 107: 225-232.
    • (2011) Plant Cell Tissue Organ Cult , vol.107 , pp. 225-232
    • Fan, J.1    Liu, X.2    Xu, S.X.3    Xu, Q.4    Guo, W.W.5
  • 11
    • 0036278859 scopus 로고    scopus 로고
    • Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream
    • Ginzinger DG (2002) Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 30: 503-512.
    • (2002) Exp Hematol , vol.30 , pp. 503-512
    • Ginzinger, D.G.1
  • 13
    • 0034953244 scopus 로고    scopus 로고
    • Quantitative teal-time PCR assay for determining transgene copy number in transformed plants
    • Ingham DJ, Beer S, Money S, Hansen G (2001) Quantitative teal-time PCR assay for determining transgene copy number in transformed plants. Biotechniques 31: 132-140.
    • (2001) Biotechniques , vol.31 , pp. 132-140
    • Ingham, D.J.1    Beer, S.2    Money, S.3    Hansen, G.4
  • 15
    • 0346319004 scopus 로고    scopus 로고
    • The relationship between homozygous and hemizygous transgene expression levels over generations in populations of transgenic rice plants
    • James VA, Avart C, Worland B, Snape JW, Vain P (2002) The relationship between homozygous and hemizygous transgene expression levels over generations in populations of transgenic rice plants. Theor Appl Genet 104: 553-561.
    • (2002) Theor Appl Genet , vol.104 , pp. 553-561
    • James, V.A.1    Avart, C.2    Worland, B.3    Snape, J.W.4    Vain, P.5
  • 17
    • 0028834086 scopus 로고
    • Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
    • Livak KJ, Flood SJ, Marmaro J, Giusti W, Deetz K (1995) Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. Genome Res 4: 357-362.
    • (1995) Genome Res , vol.4 , pp. 357-362
    • Livak, K.J.1    Flood, S.J.2    Marmaro, J.3    Giusti, W.4    Deetz, K.5
  • 18
    • 73649109590 scopus 로고    scopus 로고
    • Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against citrus tristeza virus in transgenic Mexican lime
    • López C, Cervera M, Fagoaga C, Moreno P, Navarro L, Flores R, Peña L (2010) Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against citrus tristeza virus in transgenic Mexican lime. Mol Plant Pathol 11: 33-41.
    • (2010) Mol Plant Pathol , vol.11 , pp. 33-41
    • López, C.1    Cervera, M.2    Fagoaga, C.3    Moreno, P.4    Navarro, L.5    Flores, R.6    Peña, L.7
  • 19
    • 0347906083 scopus 로고    scopus 로고
    • Estimating the number of integrations in transformed plants by quantitative real-time PCR
    • Mason G, Provero P, Vaira AM, Accotto GP (2002) Estimating the number of integrations in transformed plants by quantitative real-time PCR. BMC Biotechnol 2: 20-30.
    • (2002) BMC Biotechnol , vol.2 , pp. 20-30
    • Mason, G.1    Provero, P.2    Vaira, A.M.3    Accotto, G.P.4
  • 20
    • 62249114100 scopus 로고    scopus 로고
    • Estimation of transgene copy number in transformed citrus plants by quantitative multiplex real-time PCR
    • Omar AA, Ddkkers MG, Graham JH, Grosser JW (2008) Estimation of transgene copy number in transformed citrus plants by quantitative multiplex real-time PCR. Biotechnol Prog 24: 1241-1248.
    • (2008) Biotechnol Prog , vol.24 , pp. 1241-1248
    • Omar, A.A.1    Ddkkers, M.G.2    Graham, J.H.3    Grosser, J.W.4
  • 21
    • 0037359194 scopus 로고    scopus 로고
    • Gene silencing in transgenic soybean plants transformed via particle bombardment
    • Reddy MS, Dinkins RD, Collins GB (2003) Gene silencing in transgenic soybean plants transformed via particle bombardment. Plant Cell Rep 21: 676-683.
    • (2003) Plant Cell Rep , vol.21 , pp. 676-683
    • Reddy, M.S.1    Dinkins, R.D.2    Collins, G.B.3
  • 22
    • 0034920032 scopus 로고    scopus 로고
    • Quantitative detection of transgenes in soybean (Glycine max (L.) Merrill) and peanut (Arachis hypogaea L.) by real-time polymerase chain reaction
    • Schmidt MA, Parrott WA (2001) Quantitative detection of transgenes in soybean (Glycine max (L.) Merrill) and peanut (Arachis hypogaea L.) by real-time polymerase chain reaction. Plant Cell Rep 20: 422-428.
    • (2001) Plant Cell Rep , vol.20 , pp. 422-428
    • Schmidt, M.A.1    Parrott, W.A.2
  • 23
    • 12744280061 scopus 로고    scopus 로고
    • Silencing in Arabidopsis T-DNA transformants: the predominant role of a gene-specific RNA sensing mechanism versus position effects
    • Schubert D, Lechtenberg B, Forsbach A, Gils M, Bahadur S, Schmidt R (2004) Silencing in Arabidopsis T-DNA transformants: the predominant role of a gene-specific RNA sensing mechanism versus position effects. Plant Cell 16: 2561-2572.
    • (2004) Plant Cell , vol.16 , pp. 2561-2572
    • Schubert, D.1    Lechtenberg, B.2    Forsbach, A.3    Gils, M.4    Bahadur, S.5    Schmidt, R.6
  • 25
    • 1842473595 scopus 로고    scopus 로고
    • Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation
    • Shou HX, Frame BR, Whitham SA, Wang K (2004) Assessment of transgenic maize events produced by particle bombardment or Agrobacterium-mediated transformation. Mol Breed 13: 201-208.
    • (2004) Mol Breed , vol.13 , pp. 201-208
    • Shou, H.X.1    Frame, B.R.2    Whitham, S.A.3    Wang, K.4
  • 27
    • 0036218905 scopus 로고    scopus 로고
    • Quantitative real-time PCR as a screening tool for estimating transgene copy number in WHISKEPRSTM-derived transgenic maize
    • Song P, Cai CQ, Skokut M, Kosegi BD (2002) Quantitative real-time PCR as a screening tool for estimating transgene copy number in WHISKEPRSTM-derived transgenic maize. Plant Cell Rep 20: 948-954.
    • (2002) Plant Cell Rep , vol.20 , pp. 948-954
    • Song, P.1    Cai, C.Q.2    Skokut, M.3    Kosegi, B.D.4
  • 28
    • 67349161319 scopus 로고    scopus 로고
    • Highly efficient transformation of GFP and KUN genes into precocious trifoliate orange (Poncirus trifoliata[L.] Raf), a potential model genopype for functional genomics studies in Citrus
    • Tan B, Li DL, Xu SX, Fan GE, Fan J, Guo WW (2009) Highly efficient transformation of GFP and KUN genes into precocious trifoliate orange (Poncirus trifoliata[L.] Raf), a potential model genopype for functional genomics studies in Citrus. Tree Genet Genomes 5: 529-537.
    • (2009) Tree Genet Genomes , vol.5 , pp. 529-537
    • Tan, B.1    Li, D.L.2    Xu, S.X.3    Fan, G.E.4    Fan, J.5    Guo, W.W.6
  • 29
    • 33847688522 scopus 로고    scopus 로고
    • Genetic transformation and gene silencing mediated by multiple copies of a transgene in easten white pine
    • Tang W, Newton RJ, Weidner DA (2007) Genetic transformation and gene silencing mediated by multiple copies of a transgene in easten white pine. J Exp Bot 58: 545-554.
    • (2007) J Exp Bot , vol.58 , pp. 545-554
    • Tang, W.1    Newton, R.J.2    Weidner, D.A.3
  • 30
    • 0029670262 scopus 로고    scopus 로고
    • Molecular beacons: probes that fluoresce upon hybridization
    • Tyagi S, Kramer FR (1996) Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 14: 303-308.
    • (1996) Nat Biotechnol , vol.14 , pp. 303-308
    • Tyagi, S.1    Kramer, F.R.2
  • 32
    • 13444311862 scopus 로고    scopus 로고
    • Estimating number of transgene copies in transgenic rapeseed by real-time PCR assay with HMG I/Y as an indogenous reference gene
    • Weng HB, Pan AH, Yang LT, Zhang CM, Liu ZL, Zhang DB (2004) Estimating number of transgene copies in transgenic rapeseed by real-time PCR assay with HMG I/Y as an indogenous reference gene. Plant Mol Biol Rep 22: 289-300.
    • (2004) Plant Mol Biol Rep , vol.22 , pp. 289-300
    • Weng, H.B.1    Pan, A.H.2    Yang, L.T.3    Zhang, C.M.4    Liu, Z.L.5    Zhang, D.B.6
  • 33
    • 0037385911 scopus 로고    scopus 로고
    • Isolation and characterization of a cDNA ecoding a lipid transfer protein expressed in 'Valencia' orange during abscission
    • Wu ZC, Burns JK (2003) Isolation and characterization of a cDNA ecoding a lipid transfer protein expressed in 'Valencia' orange during abscission. J Exp Bot 54: 1183-1191.
    • (2003) J Exp Bot , vol.54 , pp. 1183-1191
    • Wu, Z.C.1    Burns, J.K.2
  • 35
    • 39749116770 scopus 로고    scopus 로고
    • Quantitative real-time PCR assay to detect transgene copy number in cotton (Gossypium hirsutum)
    • Yi CX, Zhang J, Chan KM, Liu XK, Hong Y (2008) Quantitative real-time PCR assay to detect transgene copy number in cotton (Gossypium hirsutum). Anal Biochem 375: 150-152.
    • (2008) Anal Biochem , vol.375 , pp. 150-152
    • Yi, C.X.1    Zhang, J.2    Chan, K.M.3    Liu, X.K.4    Hong, Y.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.