The relationship between homozygous and hemizygous transgene expression levels over generations in populations of transgenic rice plants

Theoretical and Applied Genetics

Volumn 104, Issue 4, 2002, Pages 553-561

  James, V A ^a   Avart, C ^b   Worland, B ^a   Snape, J W ^a   Vain, Philippe ^a  

^a JOHN INNES CENTRE   (United Kingdom)

^b UNIVERSITÉ DE TOULOUSE   (France)

Author keywords

Homozygous; Matrix attachment regions (MARs); Rice; Transformation; Transgene dosage

Indexed keywords

BIOASSAY; GENES; MOLECULAR BIOLOGY; STATISTICAL METHODS;

TRANSGENE EXPRESSIONS;

PLANTS (BOTANY);

EMBRYOPHYTA; EUKARYOTA;

EID: 0346319004     PISSN: 00405752     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001220100745     Document Type: Article

References (39)

1. Aida R, Shibata M (1998) Developmentally regulated transgene silencing in Torenia. Breeding Sci 48:63-69
2. Allen GC, Hall GE Jr, Michalowski S, Newman W, Spiker S, Weissinger AK, Thompson WF (1996) High-level transgene expression in plant cells: Effects of a strong scaffold attachment region from tobacco. Plant Cell 8:899-913
3. Allen GC, Spiker S, Thompson WF (2000) Use of matrix attachment regions (MARs) to minimize transgene silencing. Plant Mol Biol 43:361-376
4. Baruah-Wolff J, Harwood, WA, Lonsdale DA, Harvey A, Hull R, Snape JW (1999) Luciferase as a reporter gene for transformation studies in rice (Oryza sativa L.). Plant Cell Rep 18:715-720
5. Bhattacharyya MK, Stermer BA, Dixon, RA (1994) Reduced variation in transgene expression from a binary vector with selectable markers at the right and left T-DNA borders. Plant J 6:957-968
6. Brandle JE, McHugh SG, James L, Labbé H, Miki BL (1995) Instability of transgene expression in field grown tobacco carrying the csr1-1 gene for sulfonylurea herbicide resistance. Bio/Technology 13:994-998
7. Breyne P, Gheysen G, Jacobs A, Van Montagu M, Depicker A (1992) Effect of T-DNA configuration on transgene expression. Mol Gen Genet 235:389-396
8. Caligari PDS, Yapabandara YMHB, Paul EM, Perret J, Roger P, Dunwell JM (1993) Field performance of derived generations of transgenic tobacco. Theor Appl Genet 86:875-879
9. Cooley J, Ford T, Chrisou P (1995) Molecular and genetic characterization of elite transgenic rice plants produced by electric-discharge particle acceleration. Theor Appl Genet 90:97-104
10. De Carvalho F, Gheysen G, Kushnir S, Van Montagu M, Inze D, Castresana C (1992) Suppression of B-1,3-glucanase transgene expression in homozygous plants. EMBO J 11:2595-2602
11. Duan X, Li X, Xue Q, Abo-El-Saad M, Xu D, Wu R (1996) Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant. Nature Biotechnol. 14:494-498
12. Elkind Y, Nir B, Nadler-Hassad T (1995) Quantitative analysis of the transgene variability among primary transformants. Transgenic Res 4:30-38
13. Fearing PL, Brown D, Vlachos D, Meghji M, Privalle L (1997) Quantitative analysis of CryIA(b) expression in Bt maize plants, tissues, and silage and stability of expression over successive generations. Mol Breed 3:169-176
14. Finnegan J, McElroy D (1994) Transgene inactivation: Plants fight back! Bio/Technology 12:883-888
15. Gordon-Kamm WJ, Spencer, TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR Jr, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP, Lemaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2:603-618
16. Hart CM., Fischer B, Neuhaus J-M, Meins F Jr (1992) Regulated inactivation of homologous gene expression in transgenic Nicotiana sylvestris plants containing a defense-related tobacco chitinase gene. Mol Gen Genet 235:179-188
17. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271-282
18. Hobbs SLA, Kpodar P, Delong CMO (1990) The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol 15:851-864
19. Iyer LM, Kumpatla SP, Chandrasekharan MB, Hall TC (2000) Transgene silencing in monocots. Plant Mol Biol 43:323-346
20. Jefferson RA, Kavanagh TA, Bevan MW (1987) β-glucuronidase as a sensitive and versatile fusion marker in higher plants. EMBO J 6:3901-3907
21. Jones L, Hamilton AJ, Voinnet O, Thomas CL, Maule AJ, Baulcombe DC (1999) RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing. Plant Cell 11:2291-2301
22. Matzke MA, Matzke, AJM (1995) How and why do plants inactivate homologous (trans)genes? Plant Physiol 107:679-685
23. Matzke MA, Mette MF, Matzke AJM (2000) Transgene silencing by the host genome defense: Implications for the evolution of epigenetic control mechanisms in plants and vertebrates. Plant Mol Biol 43:401-415
24. Meyer P (1998) Stabilities and instabilities in transgene expression. In: Lindsey K (ed) Transgenic Plant Research. Harwood Academic, Amsterdam, pp 263-275
25. Mlynárová L, Loonen A, Heldens J, Jansen RC, Keizer P, Stiekema WJ, Nap J-P (1994) Reduced position-effect in mature transgenic plants conferred by the chicken lysozyme matrix associated region. Plant Cell 6:417-426
26. Mlynárová L, Keizer P, Stiekema WJ, Nap J-P (1996) Approaching the lower limits of transgene variability. Plant Cell 8:1589-1599
27. Neuhaus JM, Ahl-Goy P, Hinz U, Flores S, Meins F Jr (1991) High-level expression of a tobacco chitinase gene in Nicotiana sylvestris. Susceptibility of transgenic plants to Cercospora nicotianae infection. Plant Mol Biol 16:141-151
28. Pang S-Z, Jan F-J, Carney K, Stout J, Tricoli DM, Quemada HD, Gonslaves D (1996) Post-transcriptional transgene silencing and consequent tospovirus resistance in transgenic lettuce are affected by transgene dosage and plant development. Plant J 9:899-909
29. Peach C, Velten, J (1991) Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Mol Biol 17:49-60
30. Peng J, Wen F, Lister RL, Hodges TK (1995) Inheritance of gusA and neo genes in transgenic rice. Plant Mol Biol 27:91-104
31. Schöffl F, Schröder G, Kleim M, Rieping M (1993) An SAR sequence containing the 395-bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plants. Transgenic Res 2:93-100
32. Scott A, Woodfield D, White DWR (1998) Allelic composition and genetic background effects on transgene expression and inheritance in white clover. Mol Breed 4:479-490
33. Snedecor GW, Cochran WG (1980) Statistical Methods, 7th Edn. The Iowa State University Press, Annes, Iowa
34. Spencer TM, O'Brien JV, Start WG, Adams TR, Gordon-Kamm WJ, Lemaux PG (1992) Segregation of transgenes in maize. Plant Mol Biol 18:201-210
35. Stell RGD, Torries JH (1980) Principles and Procedures of Statistics: A Biometrical Approach, 2nd Edn. (McGraw-Hill, New York)
36. Ülker B, Allen GC, Thompson WF, Spiker S, Weissinger AK (1999) A tobacco MAR increases transgene expression and protects against gene silencing in the progeny of transgenic tobacco plants. Plant J 18:253-263
37. Vain P, Worland B, Clarke MC, Richard G, Beavis M, Liu H, Kohli A, Leech M, Snape, J, Christou P, Atkinson H (1998) Expression of an engineered proteinase inhibitor (Oryzacystatin-IΔd86) for nematode resistance in transgenic rice plants. Theor Appl Genet 96:266-271
38. Vain P, Worland B, Kohli A, Snape JW, Christou P, Allen GC, Thompson WF (1999) Matrix attachment regions increase transgene expression levels and stability in transgenic rice plants and their progeny. Plant J 18:233-242
39. Walters DA, Vetsch CS, Potts DE, Lundquist RC (1992) Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Plant Mol Biol 18:189-200