HcRed, a genetically encoded fluorescent binary cross-linking agent for cross-linking of mitochondrial ATP synthase in saccharomyces cerevisiae

PLoS ONE

Volumn 7, Issue 4, 2012, Pages

  Gong, Lan ^a   Ramm, Georg ^a   Devenish, Rodney J ^a   Prescott, Mark ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CROSS LINKING REAGENT; DIMER; HCRED; MITOCHONDRIAL ADENOSINE TRIPHOSPHATE SYNTHASE; MONOMER; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; UNCLASSIFIED DRUG; PHOTOPROTEIN; RED FLUORESCENT PROTEIN;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; MITOCHONDRIAL CRISTAE; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; NONHUMAN; PROTEIN ASSEMBLY; PROTEIN CROSS LINKING; SACCHAROMYCES CEREVISIAE; YEAST CELL; CHEMISTRY; CYTOLOGY; DIMERIZATION; DRUG EFFECT; ENZYMOLOGY;

SACCHAROMYCES CEREVISIAE;

CROSS-LINKING REAGENTS; DIMERIZATION; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; MITOCHONDRIAL MEMBRANES; MITOCHONDRIAL PROTON-TRANSLOCATING ATPASES; SACCHAROMYCES CEREVISIAE;

EID: 84859315915     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0035095     Document Type: Article

References (31)

1. Pastuszka MK, Mackay JA, (2011) Biomolecular engineering of intracellular switches in eukaryotes. J Drug Deliv Sci Technol 20: 163-169.
2. Chudakov DM, Matz MV, Lukyanov S, Lukyanov KA, (2011) Fluorescent proteins and their applications in imaging living cells and tissues. Physiol Rev 90: 1103-1163.
3. Gurskaya NG, Fradkov AF, Terskikh A, Matz MV, Labas YA, et al. (2001) GFP-like chromoproteins as a source of far-red fluorescent proteins. FEBS Lett 507: 16-20.
4. Fradkov AF, Verkhusha VV, Staroverov DB, Bulina ME, Yanushevich YG, et al. (2002) Far-red fluorescent tag for protein labelling. Biochem J 368: 17-21.
5. Wilmann PG, Petersen J, Pettikiriarachchi A, Buckle AM, Smith SC, et al. (2005) The 2.1A crystal structure of the far-red fluorescent protein HcRed: inherent conformational flexibility of the chromophore. J Mol Biol 349: 223-237.
6. Davies KM, Strauss M, Daum B, Kief JH, Osiewacz HD, et al. (2011) Macromolecular organization of ATP synthase and complex I in whole mitochondria. Proc Natl Acad Sci U S A 108: 14121-14126.
7. Seelert H, Dencher NA, (2011) ATP synthase superassemblies in animals and plants: Two or more are better. Biochim Biophys Acta 1807: 1185-1197.
8. Allen RD, Schroeder CC, Fok AK, (1989) An investigation of mitochondrial inner membranes by rapid-freeze deep-etch techniques. J Cell Biol 108: 2233-2240.
9. Arnold I, Pfeiffer K, Neupert W, Stuart RA, Schagger H, (1998) Yeast mitochondrial F1F0-ATP synthase exists as a dimer: identification of three dimer-specific subunits. Embo J 17: 7170-7178.
10. Paumard P, Vaillier J, Coulary B, Schaeffer J, Soubannier V, et al. (2002) The ATP synthase is involved in generating mitochondrial cristae morphology. Embo J 21: 221-230.
11. Gavin PD, Prescott M, Luff SE, Devenish RJ, (2004) Cross-linking ATP synthase complexes in vivo eliminates mitochondrial cristae. J Cell Sci 117: 2333-2343.
12. Gavin P, Devenish RJ, Prescott M, (2002) An approach for reducing unwanted oligomerisation of DsRed fusion proteins. Biochem Biophys Res Commun 298: 707-713.
13. Straffon AF, Prescott M, Nagley P, Devenish RJ, (1998) The assembly of yeast mitochondrial ATP synthase: subunit depletion in vivo suggests ordered assembly of the stalk subunits b, OSCP and d. Biochim Biophys Acta 1371: 157-162.
14. Prescott M, Nowakowski S, Gavin P, Nagley P, Whisstock JC, et al. (2003) Subunit gamma-green fluorescent protein fusions are functionally incorporated into mitochondrial F1F0-ATP synthase, arguing against a rigid cap structure at the top of F1. J Biol Chem 278: 251-256.
15. Bevis BJ, Glick BS, (2002) Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat Biotechnol 20: 83-87.
16. Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, et al. (2002) A monomeric red fluorescent protein. Proc Natl Acad Sci U S A 99: 7877-7882.
17. Prescott M, Lourbakos A, Bateson M, Boyle G, Nagley P, et al. (1997) A novel fluorescent marker for assembled mitochondria ATP synthase of yeast. OSCP subunit fused to green fluorescent protein is assembled into the complex in vivo. FEBS Lett 411: 97-101.
18. Gietz RD, Schiestl RH, (2007) High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2: 31-34.
19. Arnold I, Folsch H, Neupert W, Stuart RA, (1998) Two distinct and independent mitochondrial targeting signals function in the sorting of an inner membrane protein, cytochrome c1. J Biol Chem 273: 1469-1476.
20. Rosado CJ, Mijaljica D, Hatzinisiriou I, Prescott M, Devenish RJ, (2008) Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy 4: 205-213.
21. Boyle GM, Roucou X, Nagley P, Devenish RJ, Prescott M, (1999) Identification of subunit g of yeast mitochondrial F1F0-ATP synthase, a protein required for maximal activity of cytochrome c oxidase. Eur J Biochem 262: 315-323.
22. Bateson M, Devenish RJ, Nagley P, Prescott M, (1999) Single copies of subunits d, oligomycin-sensitivity conferring protein, and b are present in the Saccharomyces cerevisiae mitochondrial ATP synthase. J Biol Chem 274: 7462-7466.
23. Schagger H, Cramer WA, von Jagow G, (1994) Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal Biochem 217: 220-230.
24. Griffith J, Mari M, De Maziere A, Reggiori F, (2008) A cryosectioning procedure for the ultrastructural analysis and the immunogold labelling of yeast Saccharomyces cerevisiae. Traffic 9: 1060-1072.
25. Liou W, Geuze HJ, Slot JW, (1996) Improving structural integrity of cryosections for immunogold labeling. Histochem Cell Biol 106: 41-58.
26. Devenish RJ, Prescott M, Rodgers AJ, (2008) The structure and function of mitochondrial F1F0-ATP synthases. Int Rev Cell Mol Biol 267: 1-58.
27. Stock D, Leslie AG, Walker JE, (1999) Molecular architecture of the rotary motor in ATP synthase. Science 286: 1700-1705.
28. Couoh-Cardel SJ, Uribe-Carvajal S, Wilkens S, Garcia-Trejo JJ, (2010) Structure of dimeric F1F0-ATP synthase. J Biol Chem 285: 36447-36455.
29. Gavin PD, Prescott M, Devenish RJ, (2005) F1F0-ATP synthase complex interactions in vivo can occur in the absence of the dimer specific subunit e. J Bioenerg Biomembr 37: 55-66.
30. Lukyanov KA, Chudakov DM, Lukyanov S, Verkhusha VV, (2005) Innovation: Photoactivatable fluorescent proteins. Nat Rev Mol Cell Biol 6: 885-891.
31. Fenno L, Yizhar O, Deisseroth K, (2011) The development and application of optogenetics. Annu Rev Neurosci 34: 389-412.