In vitro cytotoxicity and morphological assessment of smoke from polymer combustion in human lung derived cells (A549)

International Journal of Hygiene and Environmental Health

Volumn 215, Issue 3, 2012, Pages 320-332

  Lestari, F ^a,b   Hayes, A J ^a   Green, A R ^a   Chattopadhyay, G ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b UNIVERSITY OF INDONESIA   (Indonesia)

Author keywords

A549; ATD GCMS; ATP assay; Cytotoxicity of thermal decomposition products; MTS assay; NRU assay; Scanning electron microscopy

Indexed keywords

POLYMER; VOLATILE ORGANIC COMPOUND;

ARTICLE; CELL CULTURE; CHEMISTRY; DRUG EFFECT; FIRE; HUMAN; LUNG; MASS FRAGMENTOGRAPHY; PATHOLOGY; POLLUTANT; SMOKE; ULTRASTRUCTURE;

CELLS, CULTURED; ENVIRONMENTAL POLLUTANTS; FIRES; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; LUNG; POLYMERS; SMOKE; VOLATILE ORGANIC COMPOUNDS;

EID: 84859209573     PISSN: 14384639     EISSN: 1618131X     Source Type: Journal    
DOI: 10.1016/j.ijheh.2011.12.006     Document Type: Article

References (71)

1. Alajbeg A. Products of non-flaming combustion of poly(vinyl chloride). Journal of Analytical and Applied Pyrolysis 1987, 12(3-4):275-291.
2. Anderson R.C., Dierdorf J., Stock M.F., Matijak M., Sawin R., Alarie Y. Use of experimental materials to assess toxicological tests for rating polymeric materials under thermal stress. Fire and Materials 1978, 2:136-140.
3. Avakian M.D, Dellinger B., Fiedler H., Gullet B., Koshland C., Marklund S., Oberdprster G., Safe S., Sarofim A., Smith K.R., Schwartz D., Suk W.A. The origin, fate and health effects of combustion by-products: a research framework. Environmental Health Perspectives 2002, 10(11):1155-1162.
4. Aufderheide M., Knebel J.W., Ritter D. Novel approaches for studying pulmonary toxicity in vitro. Toxicology Letters 2003, 140-141:205-211.
5. Bakand S., Hayes A., Winder C., Khalil C., Markovic B. In vitro cytotoxicity testing of airborne formaldehyde collected in serum-free culture media. Toxicology and Inhalation Health 2005, 21:1-8.
6. Bakand S., Winder C., Khalil C., Hayes A. Toxicity assessment of industrial chemicals and airborne contaminants: transition from in vivo to in vitro test methods: a review. Inhalation Toxicology 2005, 17:775-787.
7. Bakand S., Winder C., Khalil C., Hayes A. An experimental in vitro model for dynamic direct exposure of human cells to airborne contaminants. Toxicology Letters 2006, 165:1-10.
8. Bakand S., Winder C., Khalil C., Hayes A. A novel in vitro exposure technique for toxicity assessment of volatile organic compounds. Journal of Environmental Monitoring 2006, 8(1):100-105.
9. Bakand S., Winder C., Hayes A. Comparative in vitro cytotoxicity assessment of selected gaseous compounds in human alveolar epithelial cells. Toxicology In Vitro 2007, 21(7):1341-1347.
10. Barrow C.S., Yves A., Stock M.F. Sensory irritation evoked by the thermal decomposition products of plasticized poly(vinyl chloride). Fire and Materials 1976, 1:147-153.
11. Blaauboer B.J. The applicability of in vitro-derived data in hazard identification and characterization of chemicals. Environmental Toxicology and Pharmacology 2002, 11:213-225.
12. Blumenstock M., Zimmermann R., Schramm K.W., Kettrup A. Identification of surrogate compounds for the emission of PCDD/F (I-TEQ value) and evaluation of their on-line real-time detectability in flue gases of waste incineration plants by REMPI-TOFMS mass spectrometry. Chemosphere 2001, 42(5-7):507-518.
13. Borenfreund, E., Babich, H., 1992. The Neutral Red Cytotoxicity Assay. Invittox. ECVAM.
14. BS 7990 Tube Furnace Method for the Determination of Toxic Product Yields in Fire Effluents 2003, British Standard Institution.
15. Caldwell D.J., Alarie Y. A method to determine the potential toxicity of smoke from burning polymers. III. Comparison of synthetic polymers to douglas fir using the UPitt II flaming combustion/toxicity of smoke apparatus. Journal of Fire Sciences 1991, 9:470-518.
16. Christmann W., Kasiske D., Kloppel K.D., Partscht H., Rotard W. Combustion of polyvinylchloride - an important source for the formation of PCDD/PCDF. Chemosphere 1989, 19(1-6):387-392.
17. Cornish H.H., Hahn K.J., Barth M.L. Experimental toxicology of pyrolysis and combustion hazards. Environmental Health Perspectives 1975, 11:191-196.
18. Cree I.A., Andreotti P.E. Measurement of cytotoxicity by ATP-based luminescence assay in primary cell cultures and cell lines. Toxicology In Vitro 1997, 11:553-556.
19. Dechsakulthorn F., Hayes A., Bakand S., Joeng L., Winder C. In vitro cytotoxicity assessment of selected nanoparticles using human skin fibroblasts. Alternatives to Animal Testing and Experimentation (AATEX) 2007, 14(Special issue):397-400.
20. Fabrellas B., Sanz P., Abad E., Rivera J., Larrazabal D. Analysis of dioxins and furans in environmental samples by GC-ion-trap MS/MS. Chemosphere 2004, 55(11):1469-1475.
21. Gandhi S., Lyon R.F. Health Hazards of Combustion Products from Aircraft Composite Materials 1998, US-DOT, FAA, Atlantic City, USA.
22. Gann, R.G., Averill, J.D., Butler, K.M., Jones, W.W., Mulholland, G.W., Neviaser, J.L., Ohlemiller, T.J., Peacock, R.D., Reneke, P.A., Hall, J.R., 2001. International study of the sublethal effects of fire smoke on survivability and health (SEFS): Phase I final report. NIST (National Institute of Standards and Technology), National Technical Information Service (NTIS).
23. Hartzell G.E. Overview of combustion toxicology. Toxicology 1996, 115:7-23.
24. Harvard, 2003. Harvard/Navicyte Diffusion/Using Systems. Harvard Apparatus, USA.
25. Hatanaka T., Kitajima A., Takeuchi M. Effect of poly(vinyl chloride) and hydrogen chloride as a chlorine source on the formation of PCDDs and PCDFs in model waste incineration. Organohalogen Compounds 2004, 66:1085-1089.
26. Hayes A. Humane Research into Air Toxicity 2007, Australasian Science.
27. Hayes A., Bakand S., Winder C. Novel in vitro exposure techniques for toxicity testing and biomonitoring of airborne contaminants. Chapter 4: emerging in vitro culture technologies. Drug Testing In Vitro-Breakthroughs and Trends in Cell Culture Technology 2007, Wiley-VCH, Berlin. U. Marx, V. Sandig (Eds.).
28. Hayes A., Markovic B. Alternatives to animal testing for determining the safety of cosmetics. Cosmetics, Aerosols and Toiletries in Australia 2000, 12:24-30.
29. Hayes A., Markovic B. Essential oils from the Australian Native Mint Bush - Prostanthera. Cosmetics, Aerosols and Toiletries in Australia 2001, 3.
30. Hayes A., Markovic B. Toxicity of Australian essential oil Backhousia citriodora (Lemon myrtle). Part 1. Antimicrobial activity and in vitro cytotoxicity. Food and Chemical Toxicology 2002, 40:535-543.
31. Hayes A.J., leach D.N., Markham J.L., Markovic B. In vitro cytotoxicity of Australian tea tree oil using human cell lines. Journal of Essential Oil Research 1997, 9:575-582.
32. Hayes A.J., Markovic B. Toxicity of Australian essential oil Backhousia citriodora (lemon myrtle). Part 2. Absorption and histopathology following application to human skin. Food and Chemical Toxicology 2003, 41:1409-1416.
33. Hinderer R.K. Limitations of using combustion toxicity testing for product selection. Journal of Vinyl and Additive Technology 1985, 7:2-7.
34. Hinderer R.K., Hirschler M.M. The toxicity of hydrogen chloride and of the smoke generated by poly(vinyl chloride), including effects on various animal species, and the implications for fire safety in characterization and toxicity of smoke. ASTM STP 1082 1990, American Society for Testing and Materials, Philadelphia. H.K. Hasegawa (Ed.).
35. Huggett C., Levin B.C. Toxicity of the pyrolysis and combustion products of poly(vinyl chlorides): a literature assessment. Fire and Materials 1987, 11:131-142.
36. ICCVAM, 2001. Report of the international workshop on in vitro methods for assessing acute systemic toxicity. Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) and National Toxicology Program (NTP) Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). National Institute of Environmental Health Sciences. NIH. DHHS.
37. Kaplan H.L. Effects of irritant gases on avoidance/escape performance and respiratory response of the baboon. Toxicology 1987, 47:165-179.
38. Kaplan H.L., Grand A.F., Hartzell G.E. Combustion Toxicology, Principles and Test Methods 1983, Technomic Publishing Company Inc., Pennsylvania.
39. Larsen J.B., Williams B.K., Nelson G.L. Pulmonary effects of combustion products from nickel-coated polycarbonate. Fire and Materials 1997, 21:213-217.
40. Lestari F., Green A.R., Chattopadhyay G., Hayes A.J. An alternative method for fire smoke toxicity assessment using human lung cells. Fire Safety Journal 2006, 41:605-615.
41. Lestari F., Hayes A.J., Green A.R., Markovic B. In vitro cytotoxicity of selected chemicals commonly produced during fire combustion using human cell lines. Toxicology In Vitro 2005, 19:653-663.
42. Lestari F., Markovic B., Green A.R., Chattopadhyay G., Hayes A.J. Comparative assessment of three in vitro exposure methods for combustion toxicity. Journal of Applied Toxicology 2006, 99-114.
43. Lestari F., Hayes A.J., Green A.R. In vitro biological toxicity assessments for fire combustion products. Fire Toxicity 2010, 346-365. Woodhead Publishing Limited, Cambridge. A. Stec, R. Hull (Eds.).
44. Lestari F., Hayes A.J., Green A.R., Chattopadhyay G. An alternative method for in vitro fire smoke toxicity assessment of polymers and composites using human lung cells. Fire and Materials 2010.
45. Levchik S.V., Weil E.D. Overview of recent developments in the flame retardancy of polycarbonate. Polymer International 2005, 54:981-998.
46. Levin B.C. A summary of the NBS literature review on the chemical nature and toxicity of the pyrolysis and combustion products from seven plastics: acrylonitrile-butadiene-styrene (ABS), nylons, polyesters, polyethylenes, polystyrenes, poly(vinyl chlorides) and rigid polyurethane foams. Fire and Materials 1987, 11:143-157.
47. Levin B.C., Fowell A.J., Birky M.M., Paabo M., Stolte A., Malek D. Further Development of a Test Method for the Assessment of the Acute Inhalation Toxicity of Combustion Products 1982, US Department of Commerce, National Bureau of Standards, Washington, DC.
48. Mcilhagger R. Smoke generation from polypropylene in different thermal environments. Fire and Materials 1989, 14:19-22.
49. Meharg A.A, Shore R.F., French M.C., Osborn D. Dioxin and furan residues in wood mice (apodemus sylvaticus) following a large scale polyvinyl chloride (PVC) fire. Environmental Pollution 1997, 97:213-220.
50. Merrill Galloway F., hirschler M.M. Transport and decay of hydrogen chloride: use of a model to predict hydrogen chloride concentrations in fires involving a room-corridor-room arrangement. Fire Safety Journal 1990, 16:33-52.
51. Michal J., Mitera J., Tardon S. Toxicity of thermal degradation products of polyethylene and polypropylene. Fire and Materials 1976, 1:160-168.
52. Nelson G.L Regulatory aspects of fire toxicology. Toxicology 1987, 47:181-199.
53. NIOSH U.S. Particulates not Otherwise Regulated, Total. 0500. NMAM (NIOSH Manual of Analytical Methods) 1994, 4th edition.
54. NIOSH, U.S., 1996. Volatile Organic Compounds (screening). 2549. NMAM (NIOSH Manual of Analytical Methods), 4th edition.
55. OECD OECD Guideline for Testing of Chemicals. 428. Skin Absorption; In Vitro Method 2004, OECD, Paris.
56. OECD OECD Guideline for Testing of Chemicals. 432. In Vitro 3T3 NRU Phototoxicity Test 2004, OECD, Paris.
57. Paabo M., Levin B.C. A literature review of the chemical nature and toxicity of the decomposition products of polyethylenes. Fire and Materials 1987, 11:55-70.
58. Promega, 2001. CellTiter-Glo™ Luminescent Cell Viability Assay. Promega. Technical Bulletin 288 (TB288). Promega. September.
59. Purser D.A. Toxicity assessment of combustion products. SFPE Handbook of Fire Protection Engineering 2002, National Fire Protection Association, Quincy, MA. 3rd edition. P.J. Dinenno, D. Drysdale, C.L. Beyler, W.D. Walton, L.P.C. Richard, J.R. Hall, J.M. Watts (Eds.).
60. Ritter D., Knebel J.W., Aufderheide M. Exposure of human lung cells to inhalable substances: a novel test strategy involving clean air exposure periods using whole diluted cigarette mainstream smoke. Inhalation Toxicology 2003, 15:67-84.
61. Ritter D., Knebel J.W., Aufderheide M. Comparative assessment of toxicities of mainstrream smoke from commercial cigarettes. Inhalation Toxicology 2004, 16:1-10.
62. Russell W.M.S., Burch R.L. The Principles of Humane Experimental Technique 1959, Methuan, London, England.
63. Smith-Hansen L., Jorgensen K.H. Combustion products from pesticides and other chemical substances determined by use of DIN 53 436. Fire Safety Journal 1994, 23:51-66.
64. Smith R. The Environmental Aspects of the Use of PVC in Building Products 1996, CSIRO Division of Chemicals and Polymers.
65. Sorathia U., Lyon R., Gann R.G., Gritzo L. Materials and fire threat. Fire Technology 1997, 33:260-275.
66. Woolley W.D., Ames S.A., Fardell P.J. Chemical aspects of combustion toxicology of fires. Fire and Materials 1979, 3:110-120.
67. Woolley W.D, Fardell P.J. Basic aspects of combustion toxicology. Fire Safety Journal 1982, 5:29-48.
68. Wunderli S., Zennegg M., Dolezal I.S., Gujer E., Moser U., Wolfensberger M., Hasler P., Noger D., Studer C., Karlaganis G. Determination of polychlorinated dibenzo-p-dioxins and dibenzo-furans in solid residues from wood combustion by HRGC/HRMS. Chemosphere 2000, 40(6):641-649.
69. Zitting A. Thermal Degradation Products of Polyethylene, Polypropylene, Polystyrene, Polyvinylchloride, and Polytetrafluoroethylene in the Processing of Plastics 1998, Nordic Council of Ministers.
70. Zitting A., Savolainen H. Neurotoxic effects of the oxidative thermal degradation products from low density polyethylene. Fire and Materials 1979, 3.
71. Zucco F., De Angelis I., Testai E., Stammati A. Toxicology investigations with cell culture systems: 20 years after. Toxicology in Vitro 2004, 18:153-163.