Efficient iPS cell production with the myod transactivation domain in serum-free culture

PLoS ONE

Volumn 7, Issue 3, 2012, Pages

  Hirai, Hiroyuki ^a   Katoku Kikyo, Nobuko ^a   Karian, Peter ^a   Firpo, Meri ^a,b   Kikyo, Nobuaki ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; MYOD PROTEIN; STAGE SPECIFIC EMBRYO ANTIGEN 4; TRANSCRIPTION FACTOR NANOG; MYOD1 MYOGENIC DIFFERENTIATION PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; POU5F1 PROTEIN, HUMAN; POU5F1 PROTEIN, MOUSE;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL REPROGRAMMING; COL6A2 GENE; COLONY FORMATION; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; FEEDER CELL; FGF7 GENE; FUSION GENE; GENE; GENE EXPRESSION; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; KLF4 GENE; MOUSE; NONHUMAN; OCT4 GENE; ONCOGENE C MYC; PLURIPOTENT STEM CELL; PROCEDURES CONCERNING CELLS; PROTEIN DOMAIN; SOX2 GENE; TERATOMA; THY1 GENE; TRANSACTIVATION; ANIMAL; CHROMATIN ASSEMBLY AND DISASSEMBLY; COCULTURE; CYTOLOGY; FIBROBLAST; FLUORESCENCE MICROSCOPY; METABOLISM; METHODOLOGY; MYOBLAST; PROTEIN TERTIARY STRUCTURE; TRANSCRIPTION INITIATION; TRANSGENIC MOUSE;

IPS;

ALKALINE PHOSPHATASE; ANIMALS; CELL CULTURE TECHNIQUES; CHROMATIN ASSEMBLY AND DISASSEMBLY; COCULTURE TECHNIQUES; CULTURE MEDIA, SERUM-FREE; FIBROBLASTS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MICE; MICE, TRANSGENIC; MICROSCOPY, FLUORESCENCE; MYOBLASTS; MYOD PROTEIN; OCTAMER TRANSCRIPTION FACTOR-3; PROTEIN STRUCTURE, TERTIARY; TERATOMA; TRANSCRIPTIONAL ACTIVATION;

EID: 84859146628     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0034149     Document Type: Article

References (16)

1. Stadtfeld M, Hochedlinger K, (2010) Induced pluripotency: history, mechanisms, and applications. Genes Dev 24: 2239-2263.
2. Takahashi K, Yamanaka S, (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126: 663-676.
3. Gonzalez F, Boue S, Izpisua Belmonte JC, (2011) Methods for making induced pluripotent stem cells: reprogramming a la carte. Nat Rev Genet 12: 231-242.
4. Hirai H, Tani T, Katoku-Kikyo N, Kellner S, Karian P, et al. (2011) Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD. Stem Cells 29: 1349-1361.
5. Hirai H, Tani T, Kikyo N, (2010) Structure and functions of powerful transactivators: VP16, MyoD and FoxA. Int J Dev Biol 54: 1589-1596.
6. Okada M, Oka M, Yoneda Y, (2010) Effective culture conditions for the induction of pluripotent stem cells. Biochim Biophys Acta 1800: 956-963.
7. Watanabe S, Hirai H, Asakura Y, Tastad C, Verma M, et al. (2011) MyoD gene suppression by Oct4 is required for reprogramming in myoblasts to produce induced pluripotent stem cells. Stem Cells 29: 505-516.
8. Wang Y, Chen J, Hu JL, Wei XX, Qin D, et al. (2011) Reprogramming of mouse and human somatic cells by high-performance engineered factors. EMBO Rep 12: 373-378.
9. Maherali N, Sridharan R, Xie W, Utikal J, Eminli S, et al. (2007) Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell 1: 55-70.
10. Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, et al. (2009) Direct cell reprogramming is a stochastic process amenable to acceleration. Nature 462: 595-601.
11. Eminli S, Foudi A, Stadtfeld M, Maherali N, Ahfeldt T, et al. (2009) Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells. Nat Genet 41: 968-976.
12. Anokye-Danso F, Trivedi CM, Juhr D, Gupta M, Cui Z, et al. (2011) Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency. Cell Stem Cell 8: 376-388.
13. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, et al. (2003) Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol 31: 1007-1014.
14. Morita S, Kojima T, Kitamura T, (2000) Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 7: 1063-1066.
15. Lengner CJ, Camargo FD, Hochedlinger K, Welstead GG, Zaidi S, et al. (2007) Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell 1: 403-415.
16. Hirai H, Verma M, Watanabe S, Tastad C, Asakura Y, et al. (2010) MyoD regulates apoptosis of myoblasts through microRNA-mediated down-regulation of Pax3. J Cell Biol 191: 347-365.