ROCK inhibitor prevents the dedifferentiation of human articular chondrocytes

Biochemical and Biophysical Research Communications

Volumn 420, Issue 1, 2012, Pages 124-129

  Matsumoto, Emi ^a   Furumatsu, Takayuki ^a   Kanazawa, Tomoko ^a   Tamura, Masanori ^a   Ozaki, Toshifumi ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

Chondrocyte; Dedifferentiation; Redifferentiation; ROCK inhibitor; SOX9; Type II collagen

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; COL2A1 PROTEIN; COLLAGEN TYPE 2; PROTEIN; PROTEOGLYCAN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; RHO KINASE INHIBITOR; SAFRANIN O; TRANSCRIPTION FACTOR SOX9; UNCLASSIFIED DRUG;

ADOLESCENT; ADULT; ARTICLE; CARTILAGE CELL; CELL CULTURE; CELL DEDIFFERENTIATION; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; GENE EXPRESSION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION;

AMIDES; CELL DIFFERENTIATION; CELLS, CULTURED; CHONDROCYTES; COLLAGEN TYPE II; ENHANCER ELEMENTS, GENETIC; GENE EXPRESSION; HUMANS; JOINTS; PROMOTER REGIONS, GENETIC; PROTEOGLYCANS; PYRIDINES; REGENERATION; RHO GTP-BINDING PROTEINS; SOX9 TRANSCRIPTION FACTOR;

EID: 84859108021     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.02.127     Document Type: Article

References (37)

1. Bedi B.T., Feeley R.J. Williams 3rd, Management of articular cartilage defects of the knee. J. Bone Joint Surg. Am. 2010, 92:994-1009.
2. Mandelbau B.R., Browne J.E., Fu F., et al. Articular cartilage lesions of the knee. Am. J. Sports Med. 1998, 26:853-861.
3. Gikas P.D., Aston W.J., Briggs T.W. Autologous chondrocyte implantation: where do we stand now?. J. Orthop. Sci. 2008, 13:283-292.
4. Benya P.D., Shaffer J.D. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 1982, 30:215-224.
5. Bonaventure J., Kadhom N., Cohen-Solal L., et al. Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. Exp. Cell Res. 1994, 212:97-104.
6. Stokes D.G., Liu G., Dharmavaram R., et al. Regulation of type-II collagen gene expression during human chondrocyte de-differentiation and recovery of chondrocyte-specific phenotype in culture involves Sry-type high-mobility-group box (SOX) transcription factors. Biochem. J. 2001, 360:461-470.
7. Takata N., Furumatsu T., Abe N., et al. Comparison between loose fragment chondrocytes and condyle fibrochondrocytes in cellular proliferation and redifferentiation. J. Orthop. Sci. 2011, 16:589-597.
8. Takahashi T., Ogasawara T., Kishimoto J., et al. Synergistic effects of FGF-2 with insulin or IGF-I on the proliferation of human auricular chondrocytes. Cell Transplant. 2005, 14:683-693.
9. Liu G., Kawaguchi H., Ogasawara T., et al. Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. J. Biol. Chem. 2007, 282:20407-20415.
10. Bishop A.L., Hall A. Rho GTPases and their effector proteins. Biochem. J. 2000, 348:241-255.
11. Burridge K., Wennerberg K. Rho and Rac take center stage. Cell 2004, 116:167-179.
12. Nobes C.D., Hall A. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 1995, 81:53-62.
13. Aspenström P. Effectors for the Rho GTPases. Curr. Opin. Cell Biol. 1999, 11:95-102.
14. Woods A., Wang G., Beier F. RhoA/ROCK signaling regulates Sox9 expression and actin organization during chondrogenesis. J. Biol. Chem. 2005, 280:11626-11634.
15. Woods A., Beier F. RhoA/ROCK signaling regulates chondrogenesis in a context-dependent manner. J. Biol. Chem. 2006, 281:13134-13140.
16. Wang G., Woods A., Sabari S., et al. RhoA/ROCK signaling suppresses hypertrophic chondrocyte differentiation. J. Biol. Chem. 2004, 279:13205-13214.
17. Date H., Furumatsu T., Sakoma Y., et al. GDF-5/7 and bFGF activate integrin α2-mediated cellular migration in rabbit ligament fibroblasts. J. Orthop. Res. 2010, 28:225-231.
18. Miyake Y., Furumatsu T., Kubota S., et al. Mechanical stretch increases CCN2/CTGF expression in anterior cruciate ligament-derived cells. Biochem. Biophys. Res. Commun. 2011, 409:247-252.
19. Furumatsu T., Shukunami C., Amemiya-Kudo M., et al. Scleraxis and E47 cooperatively regulate the Sox9-dependent transcription. Int. J. Biochem. Cell Biol. 2010, 42:148-156.
20. Furumatsu T., Kanazawa T., Yokoyama Y., et al. Inner meniscus cells maintain higher chondrogenic phenotype compared with outer meniscus cells. Connect. Tissue Res. 2011, 52:459-465.
21. Tetsunaga T., Furumatsu T., Abe N., et al. Mechanical stretch stimulates integrin αVβ3-mediated collagen expression in human anterior cruciate ligament cells. J. Biomech. 2009, 42:2097-2103.
22. Kanazawa T., Furumatsu T., Hachioji M., et al. Mechanical stretch enhances COL2A1 expression on chromatin by inducing SOX9 nuclear translocalization in inner meniscus cells. J. Orthop. Res. 2012, 30:468-474.
23. Saiga K., Furumatsu T., Yoshida A., et al. Combined use of bFGF and GDF-5 enhances the healing of medial collateral ligament injury. Biochem. Biophys. Res. Commun. 2010, 402:329-334.
24. Furumatsu T., Tsuda M., Taniguchi N., et al. Smad3 induces chondrogenesis through the activation of SOX9 via CREB-binding protein/p300 recruitment. J. Biol. Chem. 2005, 280:8343-8350.
25. Furumatsu T., Hachioji M., Saiga K., et al. Anterior cruciate ligament-derived cells have high chondrogenic potential. Biochem. Biophys. Res. Commun. 2010, 391:1142-1147.
26. Grogan S.P., Barbero A., Winkelmann V., et al. Visual histological grading system for the evaluation of in vitro-generated neocartilage. Tissue Eng. 2006, 12:2141-2149.
27. Kamachi Y., Uchikawa M., Kondoh H. Pairing SOX off: with partners in the regulation of embryonic development. Trends Genet. 2000, 16:182-187.
28. Furumatsu T., Asahara H. Histone acetylation influences the activity of Sox9-related transcriptional complex. Acta Med. Okayama 2010, 64:351-357.
29. Furumatsu T., Ozaki T., Asahara H. Smad3 activates the Sox9-dependent transcription on chromatin. Int. J. Biochem. Cell Biol. 2009, 41:1198-1204.
30. Wagner T., Wirth J., Meyer J., et al. Autosomal sex reversal and campomelic dysplasia are caused by mutations in and around the SRY-related gene SOX9. Cell 1994, 79:1111-1120.
31. Akiyama H., Lyons J.P., Mori-Akiyama Y., et al. Interactions between Sox9 and β-catenin control chondrocyte differentiation. Genes Dev. 2004, 18:1072-1087.
32. Furumatsu T., Ozaki T. Epigenetic regulation in chondrogenesis. Acta Med. Okayama 2010, 64:155-161.
33. Ikeda T., Kawaguchi H., Kamekura S., et al. Distinct roles of Sox5, Sox6, and Sox9 in different stages of chondrogenic differentiation. J. Bone Miner. Metab. 2005, 23:337-340.
34. Wegner M. All purpose Sox: the many roles of Sox proteins in gene expression. Int. J. Biochem. Cell Biol. 2010, 42:381-390.
35. Haudenschild D.R., Chen J., Pang N., et al. Rho kinase-dependent activation of SOX9 in chondrocytes. Arthritis Rheum. 2010, 62:191-200.
36. Kumar D., Lassar A.B. The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization. Mol. Cell. Biol. 2009, 29:4262-4273.
37. Appleton C.T., Usmani S.E., Mort J.S., et al. Rho/ROCK and MEK/ERK activation by transforming growth factor-α induces articular cartilage degradation. Lab. Invest. 2010, 90:20-30.