A further study on chromosome minimization by protoplast fusion in Aspergillus oryzae

Molecular Genetics and Genomics

Volumn 287, Issue 2, 2012, Pages 177-187

  Hara, Seiichi ^a   Jin, Feng Jie ^a   Takahashi, Tadashi ^a   Koyama, Yasuji ^a  

^a Noda Institute for Scientific Research   (Japan)

Author keywords

Aspergillus oryzae; Chromosome engineering; Fusants; Mutant

Indexed keywords

ARTICLE; ASPERGILLUS ORYZAE; CELL NUCLEUS; CHROMOSOME 7; CHROMOSOME 8; CHROMOSOME MINIMIZATION; CHROMOSOME STRUCTURE; COMPARATIVE GENOMIC HYBRIDIZATION; DIPLOIDY; FUNGAL STRAIN; GENETIC ENGINEERING; HAPLOIDY; HETEROZYGOTE; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTOPLAST; PROTOPLAST FUSION; PULSED FIELD GEL ELECTROPHORESIS;

ASPERGILLUS ORYZAE; BENOMYL; CELL FUSION; CELL NUCLEUS; CHROMOSOME DELETION; CHROMOSOMES, FUNGAL; COMPARATIVE GENOMIC HYBRIDIZATION; DIPLOIDY; DNA, FUNGAL; ELECTROPHORESIS, GEL, PULSED-FIELD; FLOW CYTOMETRY; FUNGICIDES, INDUSTRIAL; GENOTYPE; HAPLOIDY; MUTATION; PHENOTYPE; PROTOPLASTS;

ASPERGILLUS ORYZAE;

EID: 84858859597     PISSN: 16174615     EISSN: 16174623     Source Type: Journal    
DOI: 10.1007/s00438-011-0669-1     Document Type: Article

References (26)

1. Anné J, Peberdy JF (1975) Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions. Arch Microbiol 105:201-205
2. Anné J, Peberdy JF (1976) Induced fusion of fungal protoplasts following treatment with polyethylene glycol. J Gen Microbiol 92:413-417
3. DeFazio LG, Stansel RM, Griffith JD, Chu G (2002) Synapsis of DNA sends by DNA-dependent protein kinase. EMBO J 21:3192-3200 (Pubitemid 34670402)
4. Ferenczy L, Kevei F, Szegedi M (1975) High frequency fusion of fungal protoplasts. Experientia 31:1028-1030
5. Ferenczy L, Kevei F, Szegedi M, Franko A, Rojik I (1976) Factors affecting high frequency fungal protoplast fusion. Experientia 32:1156-1158
6. Goryshin IY, Naumann TA, Apodaca J, Reznikoff WS (2003) Chromosomal deletion formation system based on Tn5 double transposition: use for making minimal genomes and essential gene analysis. Genome Res 13:644-653 (Pubitemid 36511923)
7. Hara S, Tsuji RF, Hatamoto O, Masuda T (2002) A simple method for enrichment of uninucleate conidia of Aspergillus oryzae. Biosci Biotechnol Biochem 66:693-696 (Pubitemid 39251037)
8. Hashimoto M, Ichimura T, Mizoguchi H, Tanaka K, Fujimitsu K, Keyamura K, Ote T, Yamakawa T, Yamazaki Y, Mori H, Katayama T, Kato J (2005) Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome. Mol Microbiol 55:137-149 (Pubitemid 40127891)
9. Horn BW, Moore GG, Carbone I (2009) Sexual reproduction in Aspergillus flavus. Mycologia 101:423-429
10. Jin FJ, Takahashi T, Utsushikawa M, Furukido T, Nishida M, Ogawa M, Tokuoka M, Koyama Y (2010) A trial of minimization of chromosome 7 in Aspergillus oryzae by multiple chromosomal deletions. Mol Genet Genomics 283:1-12
11. Jones JM, Gellert M, Yang W (2001) A Ku bridge over broken DNA. Structure 9:881-884 (Pubitemid 32972154)
12. Kato J, Hashimoto M (2007) Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome. Methods Mol Biol 416:279-294
13. Kevei F, Peberdy JF (1977) Interspecific hybridization between Aspergillus nidulans and Aspergillus rugulosus by fusion of somatic protoplasts. J Gen Microbiol 102:255-262 (Pubitemid 8207997)
14. Kevei F, Peberdy JF (1979) Induced segregation in interspecific hybrids of Aspergillus nidulans and Aspergillus rugulosus obtained by protoplast fusion. Mol Gen Genet 170:213-218 (Pubitemid 9252622)
15. Kobayashi T, Abe K, Asai K, Gomi K, Juvvadi PR, Kato M, Kitamoto K, Takeuchi M, Machida M (2007) Genomics of Aspergillus oryzae. Biosci Biotechnol Biochem 71:646-670 (Pubitemid 46486623)
16. Kolisnychenko V, Plunkett G, Herring CD, Feher T, Posfai J, Blattner FB, Posfai G (2002) Engineering a reduced Escherichia coli genome. Genome Res 12:640-647 (Pubitemid 34308643)
17. Machida M, Asai K, Sano M, Tanaka T, Kumagai T, Terai G, Kusumoto K, Arima T, Akita O, Kashiwagi Y, Abe K, Gomi K, Horiuchi H, Kitamoto K, Kobayashi T, Takeuchi M, Denning DW, Galagan JE, Nierman WC, Yu J, Archer DB, Bennett JW, Bhatnagar D, Cleveland TE, Fedorova ND, Gotoh O, Horikawa H, Hosoyama A, Ichinomiya M, Igarashi R, Iwashita K, Juvvadi PR, Kato M, Kato Y, Kin T, Kokubun A, Maeda H, Maeyama N, Maruyama J, Nagasaki H, Nakajima T, Oda K, Okada K, Paulsen I, Sakamoto K, Sawano T, Takahashi M, Takase K, Terabayashi Y, Wortman JR, Yamada O, Yamagata Y, Anazawa H, Hata Y, Koide Y, Komori T, Koyama Y, Minetoki T, Suharnan S, Tanaka A, Isono K, Kuhara S, Ogasawara N, Kikuchi H (2005) Genome sequencing and analysis of Aspergillus oryzae. Nature 438:1157-1161 (Pubitemid 41831685)
18. Murakami K, Tao E, Ito Y, Sugiyama M, Kaneko Y, Harashima S, Sumiya T, Nakamura A, Nishizawa M (2007) Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism. Appl Microbiol Biotechnol 75:589-597 (Pubitemid 46776547)
19. Ninomiya Y, Suzuki K, Ishii C, Inoue H (2004) Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining. Proc Natl Acad Sci USA 101:12248-12253 (Pubitemid 39145429)
20. O'Gorman CM, Fuller HT, Dyer PS (2009) Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus. Nature 457:471-474
21. Takahashi T, Hatamoto O, Koyama Y, Abe K (2004) Efficient gene disruption in the koji-mold Aspergillus sojae using a novel variation of the positive-negative method. Mol Genet Genomics 272:344-352 (Pubitemid 39506568)
22. Takahashi T, Masuda T, Koyama Y (2006) Enhanced gene targeting frequency in ku70 and ku80 disruption mutants of Aspergillus sojae and Aspergillus oryzae. Mol Genet Genomics 275:460-470 (Pubitemid 43725263)
23. Takahashi T, Jin FJ, Sunagawa M, Machida M, Koyama Y (2008) Generation of large chromosomal deletions in koji molds Aspergillus oryzae and Aspergillus sojae via a loop-out recombination. Appl Environ Microbiol 74:7684-7693
24. Takahashi T, Jin FJ, Koyama Y (2009) Nonhomologous end-joining deficiency allows large chromosomal deletions to be produced by replacement-type recombination in Aspergillus strains. Fungal Genet Biol 46:815-824
25. Yu BJ, Sung BH, Koob MD, Lee CH, Lee JH, Lee WS et al (2002) Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system. Nat Biotechnol 20: 1018-1102
26. Yu JH, Hamari Z, Han KH, Seo JA, Reyes-Domínguez Y, Scazzocchio C (2004) Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet Biol 41:973-981 (Pubitemid 39304095)