Screening of a highly soluble and oxygen-independent blue fluorescent protein from metagenome

Biochemical and Biophysical Research Communications

Volumn 419, Issue 4, 2012, Pages 676-681

  Hwang, Chang Sun ^a   Choi, Eun Sil ^a   Han, Sang Soo ^a   Kim, Geun Joong ^a  

^a Chonnam National University   (South Korea)

Author keywords

Blue fluorescent protein; Cell imaging; NADPH; Reporter

Indexed keywords

BLUE FLUORESCENT PROTEIN; GENOMIC DNA; METAGENOMIC DNA; OXYGEN; PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

ANAEROBIC CAPACITY; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; GENE OVEREXPRESSION; METAGENOME; NONHUMAN; PRIORITY JOURNAL; SCREENING;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; FUNGI; GENE LIBRARY; GENES, REPORTER; GENETIC VECTORS; LUMINESCENT PROTEINS; METAGENOME; MOLECULAR SEQUENCE DATA; OXYGEN; PLANT LEAVES; RECOMBINANT PROTEINS; SELECTION, GENETIC; SOLUBILITY;

ESCHERICHIA COLI;

EID: 84858751576     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.02.075     Document Type: Article

References (17)

1. Cabantous S., Waldo G.S. In vivo and in vitro protein solubility assays using split GFP. Nat. Methods 2006, 3:845-854.
2. March J.C., Rao G., Bentley W.E. Biotechnological applications of green fluorescent protein. Appl. Microbiol. Biotechnol. 2003, 62:303-315.
3. Chalfie M., Tu Y., Euskirchen G., Ward W.W., Prasher D.C. Green fluorescent protein as a marker for gene expression. Science 1994, 263:802-805.
4. Cody C.W., Prasher D.C., Westler W.M., Prendergast F.G., Ward W.W. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry 1993, 32:1212-1218.
5. Drepper T., Eggert T., Circolone F., Heck A., Krauss U., Guterl J.K., Wendorff M., Losi A., Gärtner W., Jaeger K.E. Reporter proteins for in vivo fluorescence without oxygen. Nat. Biotechnol. 2007, 25:443-445.
6. Han S.S., Lee J.Y., Kim W.H., Shin H.J., Kim G.J. Screening of promoters from metagenomic DNA and their use for the construction of expression vectors. J. Microbiol. Biotechnol. 2008, 18:1634-1640.
7. Uchiyama T., Abe T., Ikemura T., Watanabe K. Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes. Nat. Biotechnol. 2005, 23:88-93.
8. Rondon M.R., August P.R., Bettermann A.D., Brady S.F., Grossman T.H., Liles M.R., Loiacono K.A., Lynch B.A., MacNeil I.A., Minor C., Tiong C.L., Gilman M., Osburne M.S., Clardy J., Handelsman J., Goodman R.M. Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms. Appl. Environ. Microbiol. 2000, 66:2541-2547.
9. Nam S.H., Oh K.H., Kim G.J., Kim H.S. Functional tuning of a salvaged green fluorescent protein variant with a new sequence space by directed evolution. Protein Eng. 2003, 16:1099-1105.
10. Lee J.Y., Jang Y.S., Lee J., Papoutsakis E.T., Lee S.Y. Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production. Biotechnol. J. 2009, 4:1432-1440.
11. Kavanagh K., Jornvall H., Persson B., Oppermann U. The SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes. Cell. Mol. Life Sci. 2008, 65:3895-3906.
12. Jornvall H., Hoog J.O., Persson B. SDR and MDR: completed genome sequences show these protein families to be large, of old origin, and of complex nature. FEBS Lett. 1999, 445:261-264.
13. Chang C.C., Chuang Y.C., Chang M.C. Fluorescent intensity of a novel NADPH-binding protein of Vibrio vulnificus can be improved by directed evolution. Biochem. Biophys. Res. Commun. 2004, 322:303-309.
14. Lakowicz J.R., Szmacinski H., Nowaczyk K., Johnson M.L. Fluorescence lifetime imaging of free and protein-bound NADH. Proc. Nat. Acad. Sci. U.S.A. 1992, 89:1271-1275.
15. Kao T.H., Chen Y., Pai C.H., Chang M.C., Wang A.H. Structure of a NADPH-dependent blue fluorescent protein revealed the unique role of Gly176 on the fluorescence enhancement. J. Struct. Biol. 2011, 174:485-493.
16. Choi E.S., Han S.S., Cheong D.E., Park M.Y., Kim J.S., Kim G.J. Generation of a fast maturating red fluorescent protein by a combined approach of elongation mutagenesis and functional salvage screening. Biochem. Biophys. Res. Commun. 2010, 391:598-603.
17. Polizzi K.M., Moore D.A., Bommarius A.S. A short-chain dehydrogenase/reductase from Vibrio vulnificus with both blue fluorescence and oxidoreductase activity. Chem. Commun. 2007, 1843-1845.