The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity

Applied Microbiology and Biotechnology

Volumn 94, Issue 1, 2012, Pages 141-150

  Brundiek, Henrike ^a   Sa, Stefan ^a   Evitt, Andrew ^a   Kourist, Robert ^a   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

CAL A type; Secreted fungal lipase; Trans fatty acid selectivity; Transmembrane domain; Ustilago maydis

Indexed keywords

CAL-A TYPE; CANDIDA ANTARCTICA; E. COLI; FUNCTIONAL EXPRESSION; LONG CHAIN FATTY ACID; MYRISTIC ACID; PH OPTIMA; RECOMBINANT EXPRESSION; TRANS FATTY ACID; TRANS-FATTY ACID SELECTIVITY; TRANS-MEMBRANE DOMAINS; USTILAGO MAYDIS;

ACTIVATION ANALYSIS; ESCHERICHIA COLI; ESTERIFICATION; ESTERS; GLUCOSE;

LIPASES;

CANDIDA ANTARCTICA LIPASE A; ESTER DERIVATIVE; FUNGAL ENZYME; GLUCOSE; LONG CHAIN FATTY ACID; MYRISTIC ACID DERIVATIVE; RECOMBINANT ENZYME; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; USTILAGO MAYDIS LIPASE UM03410;

COLIFORM BACTERIUM; ENZYME ACTIVITY; ESTER; FATTY ACID; FUNGUS; GLUCOSE; MEMBRANE; OPTIMIZATION; PH; RECOMBINATION;

AMINO TERMINAL SEQUENCE; ARTICLE; CANDIDA ANTARCTICA; CONTROLLED STUDY; CULTURE MEDIUM; ENRICHMENT CULTURE; ENZYME ACTIVITY; ESCHERICHIA COLI; LOW TEMPERATURE; NONHUMAN; PH; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; SPECIES CULTIVATION; USTILAGO MAYDIS;

ENZYME STABILITY; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; KINETICS; LIPASE; PLANT DISEASES; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY; TRANS FATTY ACIDS; USTILAGO;

CANDIDA ANTARCTICA; FUNGI; SPORISORIUM REILIANUM; USTILAGO MAYDIS;

EID: 84858451394     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-3903-9     Document Type: Article

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