Directed Mutagenesis of Specific Active Site Residues on Fibrobacter succinogenes 1,3-1,4-β-D-Glucanase Significantly Affects Catalysis and Enzyme Structural Stability

Journal of Biological Chemistry

Volumn 276, Issue 21, 2001, Pages 17895-17901

  Chen, Jui Lin ^a   Tsai, Li Chu ^b   Wen, Tuan Nan ^c   Tang, Jyh Bing ^a   Yuan, Hanna S ^b   Shyur, Lie Fen ^a  

^a AGRICULTURAL BIOTECHNOLOGY RESEARCH CENTER   (Taiwan)

^b INSTITUTE OF MOLECULAR BIOLOGY   (Taiwan)

^c INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYSIS; ENZYME KINETICS; GENETIC ENGINEERING; MOLECULAR STRUCTURE; MUTAGENESIS;

DENATURATION; MUTANTS;

BIOCHEMISTRY;

1,3:1,4 BETA DEXTRO GLUCANASE; AMINO ACID; ASPARTIC ACID; BACTERIAL ENZYME; CARBOXYL GROUP; FUNGAL ENZYME; GLUCAN GLUCOSIDASE; GLUTAMINE; GLYCINE; METHIONINE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; GLYCOSIDASE; LICHENINASE;

ARTICLE; BACILLUS; CATALYSIS; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; FIBROBACTER SUCCINOGENES; FLUORESCENCE SPECTROSCOPY; KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; WILD TYPE; AMINO ACID SEQUENCE; ANIMAL; ENZYMOLOGY; GENETICS; GRAM NEGATIVE ANAEROBIC BACTERIA; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; MUTATION; RUMINANT STOMACH; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS); FELIS CATUS; FIBROBACTER; FIBROBACTER SUCCINOGENES;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; CATALYSIS; ENZYME STABILITY; GLYCOSIDE HYDROLASES; GRAM-NEGATIVE ANAEROBIC BACTERIA; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; RUMEN; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0035947640     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M100843200     Document Type: Article

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