In vivo screening of ligand-dependent hammerhead ribozymes

Methods in Molecular Biology

Volumn 848, Issue , 2012, Pages 455-463

  Saragliadis, Athanasios ^a   Klauser, Benedikt ^a   Hartig, Jörg S ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

Allosteric; Aptamer; Aptazyme; Artificial; Functional RNA; HHR; Ligand; Riboswitch; Ribozyme; Screening

Indexed keywords

HAMMERHEAD RIBOZYME; LIGAND; RIBOZYME;

ARTICLE; BINDING SITE; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETIC CODE; GENETIC ENGINEERING; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RIBOSWITCH; SYNTHETIC BIOLOGY;

BASE SEQUENCE; BINDING SITES; ESCHERICHIA COLI; GENETIC CODE; GENETIC ENGINEERING; LIGANDS; POLYMERASE CHAIN REACTION; RIBOSWITCH; RNA, CATALYTIC; SUBSTRATE SPECIFICITY; SYNTHETIC BIOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84858254797     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-545-9_28     Document Type: Article

References (15)

1. Roth, A., and Breaker, R. R. (2009) The structural and functional diversity of metabolite-binding riboswitches, Annu Rev Biochem 78, 305-334.
2. Wieland, M., and Hartig, J. S. (2008) Artificial riboswitches: synthetic mRNA-based regulators of gene expression, Chembiochem 9, 1873-1878.
3. Tang, J., and Breaker, R. R. (1997) Rational design of allosteric ribozymes, Chem Biol 4, 453-459. (Pubitemid 27288710)
4. Khvorova, A., Lescoute, A., Westhof, E., and Jayasena, S. D. (2003) Sequence elements outside the hammerhead ribozyme catalytic core enable intracellular activity, Nat Struct Biol 10, 708-712. (Pubitemid 37052408)
5. Yen, L., Svendsen, J., Lee, J. S., Gray, J. T., Magnier, M., Baba, T., D'Amato, R. J., and Mulligan, R. C. (2004) Exogenous control of mammalian gene expression through modulation of RNA self-cleavage, Nature 431, 471-476. (Pubitemid 39329588)
6. Win, M. N., and Smolke, C. D. (2007) A modular and extensible RNA-based gene-regulatory platform for engineering cellular function, Proc Natl Acad Sci USA 104, 14283-14288. (Pubitemid 350003254)
7. Wieland, M., and Hartig, J. S. (2008) Improved aptazyme design and in vivo screening enable riboswitching in bacteria, Angew Chem Int Ed Engl 47, 2604-2607.
8. Wieland, M., Benz, A., Klauser, B., and Hartig, J. S. (2009) Artificial ribozyme switches containing natural riboswitch aptamer domains, Angew Chem Int Ed Engl 48, 2715-2718.
9. Kumar, D., An, C. I., and Yokobayashi, Y. (2009) Conditional RNA interference mediated by allosteric ribozyme, J Am Chem Soc131, 13906-13907.
10. Ogawa, A., and Maeda, M. (2008) An artificial aptazyme-based riboswitch and its cascading system in E. coli, Chembiochem 9, 206-209. (Pubitemid 351196787)
11. Auslander, S., Ketzer, P., and Hartig, J. S. (2010) A ligand-dependent hammerhead ribozyme switch for controlling mammalian gene expression, Mol Biosyst 6, 807-814.
12. Wieland, M., Gfell, M., and Hartig, J. S. (2009) Expanded hammerhead ribozymes containing addressable three-way junctions, RNA 15, 968-976.
13. Berschneider, B., Wieland, M., Rubini, M., and Hartig, J. S. (2009) Small-molecule-dependent regulation of transfer RNA in bacteria, Angew Chem Int Ed Engl 48, 7564-7567.
14. Wieland, M., Berschneider, B., Erlacher, M. D., and Hartig, J. S. (2010) Aptazymemediated regulation of 16S ribosomal RNA, Chem Biol 17, 236-242.
15. Reetz, M. T., Kahakeaw, D., and Lohmer, R. (2008) Addressing the numbers problem in directed evolution, Chembiochem 9, 1797-1804.