Investigating the kinetic mechanism of inhibition of elongation factor 2 kinase by NH125: Evidence of a common in vitro artifact

Biochemistry

Volumn 51, Issue 10, 2012, Pages 2100-2112

  Devkota, Ashwini K ^a   Tavares, Clint D J ^a   Warthaka, Mangalika ^a   Abramczyk, Olga ^a   Marshall, Kyle D ^a   Kaoud, Tamer S ^a   Gorgulu, Kivanc ^b   Ozpolat, Bulent ^b   Dalby, Kevin N ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTI-CANCER THERAPIES; ATP BINDING; BEST FIT; BREAST CANCER; CELL LINES; CELLULAR ENVIRONMENT; DOSE-RESPONSE CURVES; ELONGATION FACTOR; EXPERIMENTAL CONDITIONS; HILL COEFFICIENT; HISTIDINE KINASE; IN-VITRO; INHIBITOR CONCENTRATION; KINASE ASSAYS; KINETIC ASSAY; KINETIC MECHANISM; LUNG CANCER; MECHANISM OF ACTION; PEPTIDE SUBSTRATES; POTENT INHIBITOR; PROTEIN KINASE; SEQUENTIAL MECHANISM; SMALL MOLECULES; STEADY-STATE KINETICS; SUBSTRATE INHIBITION; WESTERN BLOTS;

AMINO ACIDS; CELL CULTURE; DISEASES; KINETICS; PEPTIDES; PHOSPHORYLATION;

ENZYMES;

1 BENZYL 3 CETYL 2 METHYLIMIDAZOLIUM IODIDE; ADENOSINE TRIPHOSPHATE; ELONGATION FACTOR 2; ELONGATION FACTOR 2 KINASE; IMIDAZOLE DERIVATIVE; NH 125; PROTEIN KINASE INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; ARTIFACT; CANCER CELL; COLLOID; COMPETITIVE INHIBITION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MECHANISM; DRUG POTENCY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INHIBITION; ENZYME KINETICS; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; IC 50; IN VITRO STUDY; PRIORITY JOURNAL; STEADY STATE; WESTERN BLOTTING;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; ARTIFACTS; CELL LINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ELONGATION FACTOR 2 KINASE; HEK293 CELLS; HUMANS; IMIDAZOLES; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDES; PHOSPHORYLATION; PROTEIN KINASE INHIBITORS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 84858249902     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi201787p     Document Type: Article

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