Nonequilibrium isotope exchange reveals a catalytically significant enzyme-phosphate complex in the ATP hydrolysis pathway of the AAA + ATPase magnesium chelatase

Biochemistry

Volumn 51, Issue 10, 2012, Pages 2029-2031

  Adams, Nathan B P ^a   Reid, James D ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ATP HYDROLYSIS; CHLOROPHYLL BIOSYNTHESIS; ISOTOPE EXCHANGE; MAGNESIUM CHELATASE; NON EQUILIBRIUM;

BIOCHEMISTRY; CHLOROPHYLL; HYDROLYSIS; ISOTOPES; MAGNESIUM;

ENZYMES;

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; MAGNESIUM CHELATASE; PHOSPHATE; PHOSPHORUS 32; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; HYDROLYSIS; ISOTOPE LABELING; NONEQUILIBRIUM ISOTOPE EXCHANGE; PRIORITY JOURNAL; RADIOASSAY;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; HYDROLYSIS; KINETICS; LYASES; MODELS, BIOLOGICAL; PHOSPHATES;

EID: 84858194935     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi300149z     Document Type: Article

References (19)

1. Houry, W. A. and Ortega, J. (2010) AAA proteins: Movers and shakers Biochem. Cell Biol. 88, i-iv
2. Tucker, P. A. and Sallai, L. (2007) The AAA+ superfamily: A myriad of motions Curr. Opin. Struct. Biol. 17, 641-652
3. Hanson, P. I. and Whiteheart, S. W. (2005) AAA+ proteins: have engine, will work Nat. Rev. Mol. Cell Biol. 6, 519-529 (Pubitemid 40943043)
4. Iyer, L. M., Leipe, D. D., Koonin, E. V., and Aravind, L. (2004) Evolutionary history and higher order classification of AAA+ ATPases J. Struct. Biol. 146, 11-31 (Pubitemid 38369015)
5. Wendler, P., Shorter, J., Snead, D., Plisson, C., Clare, D. K., Lindquist, S., and Saibil, H. R. (2009) Motor mechanism for protein threading through Hsp104 Mol. Cell 34, 81-92
6. Glynn, S. E., Martin, A., Nager, A. R., Baker, T. A., and Sauer, R. T. (2009) Structures of asymmetric ClpX hexamers reveal nucleotide-dependent motions in a AAA+ protein-unfolding machine Cell 139, 744-756
7. Zhang, X. and Wigley, D. B. (2008) The 'glutamate switch' provides a link between ATPase activity and ligand binding in AAA+ proteins Nat. Struct. Mol. Biol. 15, 1223-1227
8. Chen, B., Sysoeva, T. A., Chowdhury, S., Guo, L., De Carlo, S., Hanson, J. A., Yang, H., and Nixon, B. T. (2010) Engagement of Arginine Finger to ATP Triggers Large Conformational Changes in NtrC1 AAA+ ATPase for Remodeling Bacterial RNA Polymerase Structure 18, 1420-1430
9. Page, A. N., George, N. P., Marceau, A. H., Cox, M. M., and Keck, J. L. (2011) Structure and biochemical activities of Escherichia coli MgsA J. Biol. Chem. 286, 12075-12085
10. Lundqvist, J., Elmlund, H., Wulff, R. P., Berglund, L., Elmlund, D., Emanuelsson, C., Hebert, H., Willows, R. D., Hansson, M., Lindahl, M., and Al-Karadaghi, S. (2010) ATP-Induced Conformational Dynamics in the AAA+ Motor Unit of Magnesium Chelatase Structure 18, 354-365
11. Viney, J., Davison, P. A., Hunter, C. N., and Reid, J. D. (2007) Direct measurement of metal-ion chelation in the active site of the AAA+ ATPase magnesium chelatase Biochemistry 46, 12788-12794 (Pubitemid 350060100)
12. Reid, J. D., Siebert, C. A., Bullough, P. A., and Hunter, C. N. (2003) The ATPase activity of the ChlI subunit of magnesium chelatase and formation of a heptameric AAA+ ring Biochemistry 42, 6912-6920 (Pubitemid 36666132)
13. Fodje, M. N., Hansson, A., Hansson, M., Olsen, J. G., Gough, S., Willows, R. D., and Al Karadaghi, S. (2001) Interplay between an AAA module and an integrin I domain may regulate the function of magnesium chelatase J. Mol. Biol. 311, 111-122 (Pubitemid 32735318)
14. 2+ insertion, and the MgATP-dependent interaction of the ChlI and ChlD subunits Biochem. J. 339, 127-134 (Pubitemid 29179299)
15. Jensen, P. E., Gibson, L. C. D., and Hunter, C. N. (1998) Determinants of catalytic activity with the use of purified I, D and H subunits of the magnesium protoporphyrin IX chelatase from Synechocystis sp. PCC6803 Biochem. J. 334, 335-344 (Pubitemid 28448576)
16. Gibson, L. C. D., Willows, R. D., Kannangara, C. G., von Wettstein, D., and Hunter, C. N. (1995) Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides: Reconstitiution of activity by combining the products of the bchH, -I and -D genes expressed in Escherichia coli Proc. Natl. Acad. Sci. U.S.A. 92, 1941-1944
17. Jencks, W. P. (1969) Catalysis in chemistry and enzymology, 60.
18. Hansson, M. and Kannangara, C. G. (1997) ATPases and phosphate exchange activities in magnesium chelatase subunits of Rhodobacter sphaeroides Proc. Natl. Acad. Sci. U.S.A. 94, 13351-13356 (Pubitemid 27518525)
19. Petersen, B. L., Kannangara, C. G., and Henningsen, K. W. (1999) Distribution of ATPase and ATP-to-ADP phosphate exchange activities in magnesium chelatase subunits of Chlorobium vibrioforme and Synechocystis PCC6803 Arch. Microbiol. 171, 146-150 (Pubitemid 29142886)