Susceptibility of the PER.C6 cell line for infection with clinical human respiratory syncytial virus isolates

Journal of Virological Methods

Volumn 181, Issue 1, 2012, Pages 37-42

  Houspie, Lieselot ^a   Keyaerts, Els ^a   Maes, Piet ^a   Van Ranst, Marc ^a  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

HEp 2; HRSV; PER.C6; Propagation

Indexed keywords

VIRUS RNA;

ARTICLE; BELGIUM; CELL LINE; CELL STRAIN PER C6; CENTRIFUGATION; CONTROLLED STUDY; HEP 2 CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INFECTION SENSITIVITY; INOCULATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; ROTATION; VIROGENESIS; VIRUS ISOLATION; VIRUS PLAQUE; VIRUS REPLICATION; VIRUS STRAIN;

BELGIUM; CELL LINE; HUMANS; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; VIRUS CULTIVATION;

HUMAN RESPIRATORY SYNCYTIAL VIRUS; HYDRANGEA RINGSPOT VIRUS;

EID: 84858156105     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.01.008     Document Type: Article

References (19)

1. Collins P.L., Crowne J.J. Respiratory syncytial virus and metapneumovirus. Fields Virology 2007, vol. 2:1602-1631. Lippincot Williams and Wilkins, Philadelphia PA.
2. Cox R.J., Madhun A.S., Hauge S., Sjursen H., Major D., Kuhne M., Hoschler K., Saville M., Vogel F.R., Barclay W., Donatelli I., Zambon M., Wood J., Haaheim L.R. A phase I clinical trial of a PER.C6 cell grown influenza H7 virus vaccine. Vaccine 2009, 27:1889-1897.
3. Hartgroves L.C., Koudstaal W., McLeod C., Moncorge O., Thompson C.I., Ellis J., Bull C., Havenga M.J., Goudsmit J., Barclay W.S. Rapid generation of a well-matched vaccine seed from a modern influenza A virus primary isolate without recourse to eggs. Vaccine 2010, 28:2973-2979.
4. Jonathan N. Diagnostic utility of BINAX NOW RSV-an evaluation of the diagnostic performance of BINAX NOW RSV in comparison with cell culture and direct immunofluorescence. Ann. Clin. Microbiol. Antimicrob. 2006, 5:13.
5. Nauwelaers D., Vijgen L., Atkinson C., Todd A., Geretti A.M., Van Ranst M., Stuyver L. Development of a real-time multiplex RSV detection assay for difficult respiratory samples, using ultrasone waves and MNAzyme technology. J. Clin. Virol. 2009, 46:238-243.
6. Ooi L.S., Ho W.S., Ngai K.L., Tian L., Chan P.K., Sun S.S., Ooi V.E. Narcissus tazetta lectin shows strong inhibitory effects against respiratory syncytial virus, influenza A (H1N1, H3N2, H5N1) and B viruses. J. Biosci. 2010, 35:95-103.
7. Paillet C., Forno G., Kratje R., Etcheverrigaray M. Suspension-Vero cell cultures as a platform for viral vaccine production. Vaccine 2009, 27:6464-6467.
8. Pau M.G., Ophorst C., Koldijk M.H., Schouten G., Mehtali M., Uytdehaag F. The human cell line PER.C6 provides a new manufacturing system for the production of influenza vaccines. Vaccine 2001, 19:2716-2721.
9. Perini A.P., Barbosa M.L., Botosso V.F., de Moraes C.T., Gillio A.E., Hens N., Stewien K.E., Durigon E.L. Comparison of HeLa-I, HEp-2 and NCI-H292 cell lines for the isolation of human respiratory syncytial virus (HRSV). J. Virol. Methods 2007, 146:368-371.
10. Pons M.W., Lambert A.L., Lambert D.M., Rochovansky O.M. Improvement of respiratory syncytial virus replication in actively growing HEp-2 cells. J. Virol. Methods 1983, 7:217-221.
11. Reis A.D., Fink M.C., Machado C.M., Paz Jde P., Oliveira R.R., Tateno A.F., Machado A.F., Cardoso M.R., Pannuti C.S. Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants. Rev. Inst. Med. Trop. Sao Paulo 2008, 50:37-40.
12. Rixon H.W., Brown C., Brown G., Sugrue R.J. Multiple glycosylated forms of the respiratory syncytial virus fusion protein are expressed in virus-infected cells. J. Gen. Virol. 2002, 83:61-66.
13. Roberts S.R., Compans R.W., Wertz G.W. Respiratory syncytial virus matures at the apical surfaces of polarized epithelial cells. J. Virol. 1995, 69:2667-2673.
14. Samina I., Havenga M., Koudstaal W., Khinich Y., Koldijk M., Malkinson M., Simanov M., Perl S., Gijsbers L., Weverling G.J., Uytdehaag F., Goudsmit J. Safety and efficacy in geese of a PER.C6-based inactivated West Nile virus vaccine. Vaccine 2007, 25:8338-8345.
15. Smith M.C., Creutz C., Huang Y.T. Detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. J. Clin. Microbiol. 1991, 29:463-465.
16. Sturgill M.A., Hughes J.H. Use of high-speed rolling to detect respiratory syncytial virus in cell culture. J. Clin. Microbiol. 1989, 27:577-579.
17. Victoria J.G., Wang C., Jones M.S., Jaing C., McLoughlin K., Gardner S., Delwart E.L. Viral nucleic acids in live-attenuated vaccines: detection of minority variants and an adventitious virus. J. Virol. 2010, 84:6033-6040.
18. Zimmer G., Trotz I., Herrler G. N-glycans of F protein differentially affect fusion activity of human respiratory syncytial virus. J. Virol. 2001, 75:4744-4751.
19. Zlateva K.T., Vijgen L., Dekeersmaeker N., Naranjo C., Van Ranst M. Subgroup prevalence and genotype circulation patterns of human respiratory syncytial virus in Belgium during ten successive epidemic seasons. J. Clin. Microbiol. 2007, 45:3022-3030.