Development of a real-time multiplex RSV detection assay for difficult respiratory samples, using ultrasone waves and MNAzyme technology

Journal of Clinical Virology

Volumn 46, Issue 3, 2009, Pages 238-243

  Nauwelaers, David ^a   Vijgen, Leen ^b   Atkinson, Claire ^c   Todd, Alison ^d   Geretti, Anna Maria ^c   Van Ranst, Marc ^e   Stuyver, Lieven ^a  

^a Virco BVBA   (Belgium)

^b TIBOTEC BVBA   (Belgium)

^c ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^d Australian Technology Park Level 4   (Australia)

^e Dep of Clin and Epid Virol   (Belgium)

Author keywords

MNAzyme; NPW processing; q RT PCR; RSV A; RSV B; Sputum processing

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; ENZYMATIC ASSAY; EXTRACTION; GENE AMPLIFICATION; INTERMETHOD COMPARISON; LUNG LAVAGE; MEDICAL TECHNOLOGY; NASOPHARYNGEAL ASPIRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY SYNCYTIAL PNEUMOVIRUS A; RESPIRATORY SYNCYTIAL PNEUMOVIRUS B; VIRUS DETECTION; VIRUS PNEUMONIA;

ANIMALS; BASE SEQUENCE; BRONCHOALVEOLAR LAVAGE FLUID; DNA PRIMERS; HUMANS; MOLECULAR SEQUENCE DATA; NASOPHARYNX; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; RATS; REPRODUCIBILITY OF RESULTS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SPUTUM;

EID: 72049097672     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2009.08.013     Document Type: Article

References (14)

1. Barenfanger J., Drake C., Leon N., Mueller T., and Troutt T. Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study. J Clin Microbiol 38 8 (2000) 2824-2828
2. Hall C.B. Respiratory syncytial virus and parainfluenza virus. N Engl J Med 344 25 (2001) 1917-1928
3. van Elden L.J., van Kraaij M.G., Nijhuis M., Hendriksen K.A., Dekker A.W., Rozenberg-Arska M., et al. Polymerase chain reaction is more sensitive than viral culture and antigen testing for the detection of respiratory viruses in adults with hematological cancer and pneumonia. Clin Infect Dis 34 2 (2002) 177-183
4. Falsey A.R., McCann R.M., Hall W.J., and Criddle M.M. Evaluation of four methods for the diagnosis of respiratory syncytial virus infection in older adults. J Am Geriatr Soc 44 1 (1996) 71-73
5. Falsey A.R., and Walsh E.E. Respiratory syncytial virus infection in elderly adults. Drugs Aging 22 7 (2005) 577-587
6. Ichiyama S., Ito Y., Sugiura F., Iinuma Y., Yamori S., Shimojima M., et al. Diagnostic value of the strand displacement amplification method compared to those of Roche Amplicor PCR and culture for detecting mycobacteria in sputum samples. J Clin Microbiol 35 12 (1997) 3082-3085
7. Vuorinen P., Miettinen A., Vuento R., and Hallstrom O. Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and Roche Amplicor Mycobacterium Tuberculosis Test. J Clin Microbiol 33 7 (1995) 1856-1859
8. Gueudin M., Vabret A., Petitjean J., Gouarin S., Brouard J., and Freymuth F. Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay. J Virol Methods 109 1 (2003) 39-45
9. Hu A., Colella M., Tam J.S., Rappaport R., and Cheng S.M. Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR. J Clin Microbiol 41 1 (2003) 149-154
10. Weinberg A., Zamora M.R., Li S., Torres F., and Hodges T.N. The value of polymerase chain reaction for the diagnosis of viral respiratory tract infections in lung transplant recipients. J Clin Virol 25 2 (2002) 171-175
11. Whiley D.M., Syrmis M.W., Mackay I.M., and Sloots T.P. Detection of human respiratory syncytial virus in respiratory samples by LightCycler reverse transcriptase PCR. J Clin Microbiol 40 12 (2002) 4418-4422
12. Persson K., Hamby K., and Ugozzoli L.A. Four-color multiplex reverse transcription polymerase chain reaction-overcoming its limitations. Anal Biochem 344 1 (2005) 33-42
13. Daniels D. New solutions make qPCR a rapidly advancing field. Genet Eng Biotechnol News 28 (2008) 7
14. Hallak L.K., Spillmann D., Collins P.L., and Peeples M.E. Glycosaminoglycan sulfation requirements for respiratory syncytial virus infection. J Virol 74 22 (2000) 10508-10513