Galactose-inducible promoters in cryptococcus neoformans var. grubii

Methods in Molecular Biology

Volumn 845, Issue , 2012, Pages 211-226

  Baker, Lorina G ^a   Lodge, Jennifer K ^b  

^a Georgia Gwinett College   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Galactose induction; Glucose repression; Mating; Native galactose promoters; qPCR

Indexed keywords

GALACTOSE;

ARTICLE; CRYPTOCOCCUS NEOFORMANS; DRUG EFFECT; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; PROMOTER REGION;

CRYPTOCOCCUS NEOFORMANS; GALACTOSE; GENE EXPRESSION REGULATION, FUNGAL; PROMOTER REGIONS, GENETIC;

CRYPTOCOCCUS NEOFORMANS VAR. GRUBII; FILOBASIDIELLA NEOFORMANS; SACCHAROMYCES CEREVISIAE;

EID: 84858141665     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-539-8_14     Document Type: Chapter

References (17)

1. Lin, X., and Heitman, J. (2006) The biology of the Cryptococcus neoformans species complex. Annu Rev Microbiol 60, 69–105.
2. del Poeta, M., Toffaletti, D. L., Rude, T. H., Sparks, S. D., Heitman, J., and Perfect, J. R. (1999) Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. Infect. Immun. 67, 1812–1820.
3. Ory, J. J., Griffith, C. L., and Doering, T. L. (2004) An efficiently regulated promoter system for Cryptococcus neoformans utilizing the CTR4 promoter. Yeast 21, 919–926.
4. Wickes, B. L., and Edman, J. C. (1995) The Cryptococcus neoformans GAL7 gene and its use as an inducible promoter. Mol Microbiol 16, 1099–1109.
5. Johnston, M. (1987) A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 51, 458–476.
6. Leloir, L. F. (1951) Enzymatic transformation of uridine diphosphate glucose into a galactose derivative. Arch Biochem 33, 186–1–90.
7. Torchia, T. E., Hamilton, R. W., Cano, C. L., and Hopper, J. E. (1984) Disruption of regulatory gene GAL80 in Saccharomyces cerevisiae: effects on carbon-controlled regulation of the galactose/melibiose pathway genes. Mol Cell Biol 4, 1521–1527.
8. Torchia, T. E., and Hopper, J. E. (1986) Genetic and molecular analysis of the GAL3 gene in the expression of the galactose/melibiose regulon of Saccharomyces cerevisiae. Genetics 113, 229–2–46.
9. Tschopp, J. F., Emr, S. D., Field, C., and Schekman, R. (1986) GAL2 codes for a mem- brane-bound subunit of the galactose permease in Saccharomyces cerevisiae. J Bacteriol 166, 313–318.
10. Davidson, R., Blankenship, J., Kraus, P., Berrios, M., Hull, C., D’Souza, C., Wang, P., and Heitman, J. (2002) A PCR-based strategy to generate integrative targeting alleles with large regions of homology. Microbiology 138, 2607–2615.
11. McDade, H., and Cox, G. (2001) A new dominant selectable marker for use in Cryptococcus neoformans. Med Mycol 39, 151–154.
12. Toffaletti, D. L., Rude, T. H., Johnston, S. A., Durack, D. T., and Perfect, J. R. (1993) Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA. J Bacteriol 175, 1405–1411.
13. Hua, J., Meyer, J., and Lodge, J. (2000) Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans. Clin Diagn Lab Immun 7, 125–128.
14. Baker, L. G., Specht, C. A., Donlin, M. J., and Lodge, J. K. (2007) Chitosan, the deacetylated form of chitin, is necessary for cell wall integrity in Cryptococcus neoformans. Eukaryot Cell 6, 855–867.
15. Fujimura, H., and Sakuma, Y. (1993) Simplified isolation of chromosomal and plasmid DNA from yeasts. Biotechniques 14, 538–540.
16. Gerik, K. J., Gary, S. L., and Burgers, P. M. J. (1997) Overproduction and affinity purification of Saccharomyces cerevisiae Replication Factor C. J Biol Chem 272, 1256–1262.
17. Ruff, J. A., Lodge, J. K., and Baker, L. G. (2009) Three galactose inducible promoters for use in C. neoformans var. grubii. FG and B 46, 9–16.