Historic and recent fragmentation coupled with altitude affect the genetic population structure of one of the world's highest tropical tree line species

Global Ecology and Biogeography

Volumn 21, Issue 4, 2012, Pages 455-464

  Hensen, Isabell ^a   Cierjacks, Arne ^b   Hirsch, Heidi ^a   Kessler, Michael ^c   Romoleroux, Katya ^d   Renison, Daniel ^e   Wesche, Karsten ^f  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^c UNIVERSITY OF ZURICH   (Switzerland)

^d PONTIFICIA UNIVERSIDAD CATÓLICA DEL ECUADOR   (Ecuador)

^e UNIVERSIDAD NACIONAL DE CÓRDOBA   (Argentina)

^f SENCKENBERG MUSEUM OF NATURAL HISTORY GÖRLITZ   (Germany)

Author keywords

AFLPs; Altitudinal gradient; Central Andes; Ecuador; Genetic structure; High mountain forests; Life stages; Polylepis incana

Indexed keywords

ALTITUDE; GENE FLOW; GENETIC DIFFERENTIATION; GENETIC ISOLATION; GENETIC STRUCTURE; HABITAT FRAGMENTATION; HETEROZYGOSITY; MONTANE FOREST; POPULATION GENETICS; SEEDLING; SPECIES DIVERSITY; TREELINE;

ANDES; CORDILLERA ORIENTAL [ECUADOR]; ECUADOR;

POLYLEPIS INCANA;

EID: 84858079918     PISSN: 1466822X     EISSN: 14668238     Source Type: Journal    
DOI: 10.1111/j.1466-8238.2011.00691.x     Document Type: Article

References (55)

1. Aguilar, R., Ashworth, L., Galetto, L. & Aizen, M.A. (2006) Plant reproductive susceptibility to habitat fragmentation: review and synthesis through a meta-analysis. Ecology Letters, 9, 968-980.
2. Aragundi, S., Hamrick, J.L. & Parker, K.C. (2011) Genetic insights into the historical distribution of Polylepis pauta (Rosaceae) in the northeastern Cordillera Oriental of Ecuador. Conservation Genetics, 12, 607-618.
3. Behling, H., Berrio, J.C. & Hooghiemstra, H. (1999) Late Quaternary pollen records from the middle Caquetá river basin in central Colombian Amazon. Palaeogeography, Palaeoclimatology, Palaeoecology, 145, 193-213.
4. Bonin, A., Ehrich, D. & Manel, S. (2007) Statistical analysis of amplified fragment length polymorphism data: a toolbox for molecular ecologists and evolutionists. Molecular Ecology, 16, 3737-3758.
5. Chepstow-Lusty, A., Bennett, J.K.D., Fjeldså, J., Kendall, A., Galiano, W. & Tupayachi Herrera, A. (1998) Tracing 4, 000 years of environmental history in the Cuzco Area, Peru, from the pollen record. Mountain Research and Development, 18, 159-172.
6. Cierjacks, A., Wesche, K. & Hensen, I. (2007) Potential lateral expansion of Polylepis forest fragments in central Ecuador. Forest Ecology and Management, 242, 477-486.
7. Cierjacks, A., Rühr, N., Wesche, K. & Hensen, I. (2008a) Effects of altitude and livestock on the regeneration of two treeline forming Polylepis species in Ecuador. Plant Ecology, 194, 207-221.
8. Cierjacks, A., Salgado, S., Wesche, K. & Hensen, I. (2008b) Post-fire population dynamics of two tree species in high-altitude Polylepis forests of central Ecuador. Biotropica, 40, 176-182.
9. Colinvaux, P.A., De Oliveira, P.E., Moreno, J.E., Miller, M.C. & Bush, M.B. (1996) A long pollen record from lowland Amazonia: forest and cooling in glacial times. Science, 274, 85-87.
10. Di Pasquale, G., Marziano, M., Impagliazzo, S., Lubritto, C., de Natale, A. & Bader, M.Y. (2008) The Holocene treeline in the northern Andes (Ecuador): first evidence from soil charcoal. Palaeogeography, Palaeoclimatology, Palaeoecology, 259, 17-34.
11. Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.
12. Dubreuil, M., Riba, M., González-Martínez, S.C., Vendramin, G.G., Sebastiani, F. & Mayol, M. (2010) Genetic effects of chronic habitat fragmentation revisited: strong genetic structure in a temperate tree, Taxus baccata (Taxaceae), with great dispersal capability. American Journal of Botany, 97, 303-310.
13. Evanno, G., Regnaut, S. & Goudet, J. (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14, 2611-2620.
14. Falush, D., Stephens, M. & Pritchard, J.K. (2007) Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molecular Ecology, 7, 574-578.
15. Fjeldså, J. (2007) The relationship between biodiversity and population centres: the high Andes region as an example. Biodiversity and Conservation, 16, 2739-2751.
16. Frankham, R., Ballou, J.D. & Briscue, D.A. (2002) Introduction to conservation genetics. Cambridge University Press, Cambridge.
17. Gosling, W.D., Hanselman, J.A., Knox, C., Valencia, B.G. & Bush, M.B. (2009) Long-term drivers of change in Polylepis woodland distribution in the central Andes. Journal of Vegetation Science, 20, 1041-1052.
18. Hamrick, J.L. (2004) Response of forest trees to global environmental changes. Forest Ecology and Management, 197, 323-335.
19. Hamrick, J.L., Godt, M.J.W. & Sherman-Broyles, S.L. (1992) Factors influencing levels of genetic diversity in woody plant species. New Forests, 6, 95-124.
20. Hertel, D. & Wesche, K. (2008) Tropical moist Polylepis stands at the treeline in east Bolivia: the effect of elevation on stand microclimate, above- and below-ground structure, and regeneration. Trees, 22, 303-315.
21. Hewitt, G.M. (2000) The genetic legacy of the Quaternary ice ages. Nature, 405, 907-913.
22. Jobbágy, E.G. & Jackson, R.B. (2000) Global controls of forest line elevation in the northern and southern hemispheres. Global Ecology and Biogeography, 9, 253-268.
23. Julio, N.B., Sobral, A., Rondan Dueñas, J., Di Rienzo, J., Renison, D. & Hensen, I. (2008) RAPD and ISSR markers indicate diminished gene flow due to recent fragmentation of Polylepis australis woodlands in central Argentina. Biochemical Systematics and Ecology, 36, 329-335.
24. Jump, A.S. & Peñuelas, J. (2006) Genetic effects of chronic habitat fragmentation in a wind-pollinated tree. Proceedings of the National Academy of Sciences USA, 103, 8096-8100.
25. Kessler, M. (1995) Present and potential distribution of Polylepis (Rosaceae) forests in Bolivia. Biodiversity and conservation of Neotropical montane forests (ed. by S.P. Churchill, H. Balslev, E. Forero and J.L. Luteyn ), pp. 281-294. New York Botanical Garden, Bronx.
26. Keygene, N.V. (2004) Nomenclature of AFLP primer enzyme combinations[Online]. Available at: (accessed 14 April 2011).
27. Kramer, A.T., Ison, J.L., Ashley, M.V. & Howe, H.F. (2008) The paradox of forest fragmentation genetics. Conservation Biology, 22, 878-885.
28. Leimu, R., Mutikainen, P., Koricheva, J. & Fischer, M. (2006) How general are positive relationships between plant population size, fitness and genetic variation? Journal of Ecology, 94, 942-952.
29. López, P.G., Tremetsberger, K., Stuessy, T.F., Gómez-González, S., Jiménez, A. & Baeza, C.M. (2010) Patterns of genetic diversity in colonizing plant species: Nassauvia lagascae var. lanata (Asteraceae: Mutisieae) on Volcán Lonquimay, Chile. American Journal of Botany, 97, 423-432.
30. Lowe, A.J., Boshier, D., Ward, M., Bacles, C.F.E. & Navarro, C. (2005) Genetic resource impacts of habitat loss and degradation; reconciling empirical evidence and predicted theory for Neotropical trees. Heredity, 95, 255-273.
31. Mantel, N.A. (1967) The detection of disease clustering and a generalized regression approach. Cancer Research, 27, 209-220.
32. Marcora, P., Hensen, I., Renison, D., Seltmann, P. & Wesche, K. (2008) The performance of Polylepis australis trees along their entire altitudinal range: implications of climate change for their conservation. Diversity and Distribution, 14, 630-636.
33. Newton, A.C., Allnutt, T.R., Gillies, A.C.M., Lowe, A.J. & Ennos, R.A. (1999) Molecular phylogeography, intraspecific variation and the conservation of tree species. Trends in Ecology and Evolution, 14, 140-145.
34. Nybom, H. & Bartish, V. (2000) Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. Perspectives in Plant Ecology, Evolution and Systematics, 3, 93-114.
35. Ohsawa, T. & Ide, Y. (2008) Global patterns of genetic variation in plant species along vertical and horizontal gradients on mountains. Global Ecology and Biogeography, 17, 152-163.
36. Oksanen, J., Kindt, R., Legendre, P., O'Hara, B., Simpson, G.L., Solymos, P.M., Stevens, H.H. & Wagner, H. (2009) Vegan: community ecology package. R package version 1.15-4. Computer program and documentation distributed by the authors. Available at: (accessed 9 April 2009).
37. Parmesan, C. & Yohe, G. (2003) A globally coherent fingerprint of climate change impacts across natural systems. Nature, 421, 37-42.
38. Pautasso, M. (2009) Geographical genetics and the conservation of forest trees. Perspectives in Plant Ecology, Evolution and Systematics, 11, 157-189.
39. Peakall, R. & Smouse, P.E. (2006) GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288-295.
40. Premoli, A.C. (2003) Isozyme polymorphisms provide evidence of clinal variation with elevation in Nothofagus pumilio. Journal of Heredity, 94, 218 - 226.
41. Pritchard, J.K., Stephens, M. & Donnelly, P. (2000) STRUCTURE software version 2.3.3. Computer program and documentation distributed by the author. Available at: (accessed 6 May 2010).
42. Purcell, J. & Brelsford, A. (2004) Reassessing the causes of decline of Polylepis, a tropical subalpine forest. Ecotropica, 10, 155-158.
43. Quiroga, M.P. & Premoli, A.C. (2007) Genetic patterns in Podocarpus parlatorei reveal the long-term persistence of cold-tolerant elements in the southern Yungas. Journal of Biogeography, 34, 447-455.
44. Quiroga, M.P. & Premoli, A.C. (2010) Genetic structure of Podocarpus nubigena (Podocarpaceae) provides evidence of Quaternary and ancient historical events. Palaeogeography, Palaeoclimatology, Palaeoecology, 285, 186-193.
45. R Development Core Team (2009) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.
46. Renison, D., Hensen, I., Suarez, R. & Cingolani, A.M. (2006) Cover and growth habit of Polylepis woodlands and shrublands in the mountains of central Argentina: human or environmental influence? Journal of Biogeography, 33, 876-887.
47. Rosenberg, N.A. (2004) Distruct: a program for the graphical display of population structure. Molecular Ecology Notes, 4, 137-138.
48. Schmidt-Lebuhn, A.N., Kessler, M. & Seltmann, P. (2007) Consequences of wind pollination in an Andean Rosaceae: genetic structure and patterns of species distribution in Polylepis Ruiz & Pav. Plant Systematics and Evolution, 266, 91-103.
49. Schmidt-Lebuhn, A.N., Fuchs, J., Hertel, D., Hirsch, H., Toivonen, J. & Kessler, M. (2010) An Andean radiation: polyploidy in the tree genus Polylepis (Rosaceae, Sanguisorbeae). Plant Biology, 12, 917-926.
50. Seltmann, P., Renison, D., Cocucci, A., Hensen, I. & Jung, K. (2007) Fragment size, pollination efficiency and reproductive success in natural populations of wind-pollinated Polylepis australis (Rosaceae) trees. Flora, 202, 547-554.
51. Sork, V.L. & Smouse, P.E. (2006) Genetic analysis of landscape connectivity in tree populations. Landscape Ecology, 21, 821-836.
52. Suarez, M.L., Renison, D., Marcora, P. & Hensen, I. (2008) Age-size-habitat relationships for Polylepis australis: dealing with endangered forest ecosystems. Biodiversity and Conservation, 17, 2617-2625.
53. World Conservation Monitoring Centre (1998) Polylepis incana. IUCN Red List of Threatened Species (IUCN). Available at: (accessed 16 May 2011).
54. Williams, J.H. & Arnold, M.L. (2001) Sources of genetic structure in the woody perennial Betula occidentalis. International Journal of Plant Sciences, 162, 1097-1109.
55. Young, A., Boyle, T. & Brown, T. (1996) The population genetic consequences of habitat fragmentation for plants. Trends in Ecology and Evolution, 11, 413-418.