Measurement of the mitochondrial membrane potential and pH gradient from the redox poise of the hemes of the bc 1 complex

Biophysical Journal

Volumn 102, Issue 5, 2012, Pages 1194-1203

  Kim, N ^a   Ripple, M O ^a   Springett, R ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; HEME; OLIGOMYCIN; PROTON; PROTON IONOPHORE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE; UBIQUINOL CYTOCHROME C REDUCTASE;

ANIMAL; ARTICLE; CELL LINE; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; ELECTRON TRANSPORT; METABOLISM; MITOCHONDRIAL MEMBRANE POTENTIAL; MOUSE; PH; STATISTICS;

ANIMALS; ATP SYNTHETASE COMPLEXES; CARBONYL CYANIDE M-CHLOROPHENYL HYDRAZONE; CELL LINE; ELECTRON TRANSPORT; ELECTRON TRANSPORT COMPLEX III; HEME; HYDROGEN-ION CONCENTRATION; MEMBRANE POTENTIAL, MITOCHONDRIAL; MICE; OLIGOMYCINS; PROTON IONOPHORES; PROTONS; STOCHASTIC PROCESSES;

EID: 84858019597     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2012.02.003     Document Type: Article

References (39)

1. A.R. Crofts, and S.W. Meinhardt M. Snozzi The role of the quinone pool in the cyclic electron-transfer chain of Rhodopseudomonas sphaeroides: a modified q-cycle mechanism Biochim. Biophys. Acta 723 1983 202 218
2. P. Mitchell Keilin's respiratory chain concept and its chemiosmotic consequences Science 206 1979 1148 1159
3. D.G. Nicholls, and M.W. Ward Mitochondrial membrane potential and neuronal glutamate excitotoxicity: mortality and millivolts Trends Neurosci. 23 2000 166 174 (Pubitemid 30151213)
4. D.G. Nicholls Simultaneous monitoring of ionophore- and inhibitor-mediated plasma and mitochondrial membrane potential changes in cultured neurons J. Biol. Chem. 281 2006 14864 14874 (Pubitemid 43855191)
5. J. Llopis, and J.M. McCaffery R.Y. Tsien Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins Proc. Natl. Acad. Sci. U S A 95 1998 6803 6808 (Pubitemid 28278061)
6. G. Azarias, and H. Perreten J.Y. Chatton Glutamate transport decreases mitochondrial pH and modulates oxidative metabolism in astrocytes J. Neurosci. 31 2011 3550 3559
7. A. Osyczka, and C.C. Moser P.L. Dutton Reversible redox energy coupling in electron transfer chains Nature 427 2004 607 612 (Pubitemid 38248474)
8. 1 complex Biochem. J. 225 1985 399 405
9. V.S. Hollis, and M. Palacios-Callender S. Moncada Monitoring cytochrome redox changes in the mitochondria of intact cells using multi-wavelength visible light spectroscopy Biochim. Biophys. Acta 1607 2003 191 202 (Pubitemid 37500845)
10. M.O. Ripple, M. Abajian, and R. Springett Cytochrome c is rapidly reduced in the cytosol after mitochondrial outer membrane permeabilization Apoptosis 15 2010 563 573
11. T. Haller, M. Ortner, and E. Gnaiger A respirometer for investigating oxidative cell metabolism: toward optimization of respiratory studies Anal. Biochem. 218 1994 338 342 (Pubitemid 24141633)
12. N. Kim, M.O. Ripple, and R. Springett Spectral components of the α-band of cytochrome oxidase Biochim. Biophys. Acta. 1807 2011 779 787
13. 1 complex Biochemistry 40 2001 12584 12590 (Pubitemid 32979710)
14. R.W. Hendler, and H.V. Westerhoff Redox interactions in cytochrome c oxidase: from the "neoclassical" toward "modern" models Biophys. J. 63 1992 1586 1604 (Pubitemid 23052094)
15. D.T. Gillespie Exact stochastic simulation of coupled chemical-reactions J. Phys. Chem. 81 1977 2340 2361
16. 1 complex Biochim. Biophys. Acta 1777 2008 1053 1059
17. C.C. Moser, and T.A. Farid P.L. Dutton Electron tunneling chains of mitochondria Biochim. Biophys. Acta 1757 2006 1096 1109 (Pubitemid 44472548)
18. 1 complex? A part-time twin Biochim. Biophys. Acta 1797 2010 1849 1857
19. 1: a new device for electron transfer in proteins Trends Biochem. Sci. 26 2001 445 451 (Pubitemid 32607487)
20. 1 complex Biochemistry 34 1995 15979 15996 (Pubitemid 3013226)
21. 1 complex Arch. Biochem. Biophys. 419 2003 198 206 (Pubitemid 37329986)
22. N. Kamo, and M. Muratsugu Y. Kobatake Membrane potential of mitochondria measured with an electrode sensitive to tetraphenyl phosphonium and relationship between proton electrochemical potential and phosphorylation potential in steady state J. Membr. Biol. 49 1979 105 121 (Pubitemid 10221193)
23. D.G. Nicholls The influence of respiration and ATP hydrolysis on the proton-electrochemical gradient across the inner membrane of rat-liver mitochondria as determined by ion distribution Eur. J. Biochem 50 1974 305 315
24. D.G. Nicholls Commentary on: 'old and new data, new issues: the mitochondrial Deltapsi' by H. Tedeschi Biochim. Biophys. Acta 1710 2005 63 66
25. + cycle Biochim. Biophys. Acta 1606 2003 23 41 (Pubitemid 37267868)
26. P. Bernardi Mitochondrial transport of cations: channels, exchangers, and permeability transition Physiol. Rev. 79 1999 1127 1155 (Pubitemid 29473313)
27. R.C. Scaduto Jr., and L.W. Grotyohann Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives Biophys. J. 76 1999 469 477 (Pubitemid 29202474)
28. M.G. Sun, and J. Williams T.G. Frey Correlated three-dimensional light and electron microscopy reveals transformation of mitochondria during apoptosis Nat. Cell Biol. 9 2007 1057 1065 (Pubitemid 47628238)
29. B. Chance, and G.R. Williams Respiratory enzymes in oxidative phosphorylation. III. The steady state J. Biol. Chem. 217 1955 409 427
30. D. Gopal, and G.S. Wilson M.A. Cusanovich Cytochrome c: ion binding and redox properties. Studies on ferri and ferro forms of horse, bovine, and tuna cytochrome c J. Biol. Chem. 263 1988 11652 11656
31. P.L. Dutton, D.F. Wilson, and C.P. Lee Oxidation-reduction potentials of cytochromes in mitochondria Biochemistry 9 1970 5077 5082
32. Z. Salamon, and G. Tollin Interaction of horse heart cytochrome c with lipid bilayer membranes: effects on redox potentials J. Bioenerg. Biomembr. 29 1997 211 221 (Pubitemid 27371722)
33. S.S. Gupte, and C.R. Hackenbrock The role of cytochrome c diffusion in mitochondrial electron transport J. Biol. Chem. 263 1988 5248 5253
34. S.S. Gupte, and C.R. Hackenbrock Multidimensional diffusion modes and collision frequencies of cytochrome c with its redox partners J. Biol. Chem. 263 1988 5241 5247
35. J.D. Cortese, and C.R. Hackenbrock Motional dynamics of functional cytochrome c delivered by low pH fusion into the intermembrane space of intact mitochondria Biochim. Biophys. Acta 1142 1993 194 202 (Pubitemid 23102182)
36. N. Kojima, and G. Palmer Further characterization of the potentiometric behavior of cytochrome oxidase. Cytochrome α stays low spin during oxidation and reduction J. Biol. Chem. 258 1983 14908 14913
37. 1 and c J. Biol. Chem. 248 1973 528 533
38. S.J. Matcher, M. Cope, and D.T. Delpy Use of the water absorption spectrum to quantify tissue chromophore concentration changes in near-infrared spectroscopy Phys. Med. Biol. 39 1994 177 196 (Pubitemid 24063707)
39. L. Yu, and J.X. Xu C.A. Yu Properties of bovine heart mitochondrial cytochrome b560 J. Biol. Chem. 262 1987 1137 1143 (Pubitemid 17028425)