Aspartic acid residue D3 critically determines Cx50 gap junction channel transjunctional voltage-dependent gating and unitary conductance

Biophysical Journal

Volumn 102, Issue 5, 2012, Pages 1022-1031

  Xin, Li ^a   Nakagawa, So ^b   Tsukihara, Tomitake ^b   Bai, Donglin ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; CONNEXIN 50; EYE PROTEIN; GAP JUNCTION PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL JUNCTION; CHANNEL GATING; CHEMISTRY; ELECTRIC CONDUCTIVITY; GENETICS; HELA CELL; HUMAN; METABOLISM; MOLECULAR GENETICS; MOUSE; MUTATION; PROTEIN TRANSPORT; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; CONNEXINS; ELECTRIC CONDUCTIVITY; EYE PROTEINS; GAP JUNCTIONS; HELA CELLS; HUMANS; ION CHANNEL GATING; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN TRANSPORT;

EID: 84858018605     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2012.02.008     Document Type: Article

References (36)

1. A.L. Harris Emerging issues of connexin channels: biophysics fills the gap Q. Rev. Biophys. 34 2001 325 472 (Pubitemid 34123306)
2. D. González, J.M. Gómez-Hernández, and L.C. Barrio Molecular basis of voltage dependence of connexin channels: an integrative appraisal Prog. Biophys. Mol. Biol. 94 2007 66 106 (Pubitemid 46782977)
3. D.C. Spray, A.L. Harris, and M.V. Bennett Gap junctional conductance is a simple and sensitive function of intracellular pH Science 211 1981 712 715 (Pubitemid 11127998)
4. A.L. Harris, D.C. Spray, and M.V. Bennett Kinetic properties of a voltage-dependent junctional conductance J. Gen. Physiol. 77 1981 95 117 (Pubitemid 11179579)
5. E. Steiner, and L. Ebihara Functional characterization of canine connexin45 J. Membr. Biol. 150 1996 153 161 (Pubitemid 26116325)
6. B. Teubner, and J. Degen K. Willecke Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein J. Membr. Biol. 176 2000 249 262 (Pubitemid 30659955)
7. J.X. Jiang, and T.W. White D.L. Paul Molecular cloning and functional characterization of chick lens fiber connexin 45.6 Mol. Biol. Cell 5 1994 363 373 (Pubitemid 24116961)
8. T.W. White, and R. Bruzzone D.A. Goodenough Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins J. Cell Biol. 125 1994 879 892 (Pubitemid 24151182)
9. M.R. Al-Ubaidi, and T.W. White R. Bruzzone Functional properties, developmental regulation, and chromosomal localization of murine connexin36, a gap-junctional protein expressed preferentially in retina and brain J. Neurosci. Res. 59 2000 813 826
10. T.W. White, and M. Srinivas R. Bruzzone Virtual cloning, functional expression, and gating analysis of human connexin31.9 Am. J. Physiol. Cell Physiol. 283 2002 C960 C970
11. M.M. Kreuzberg, and G. Söhl F.F. Bukauskas Functional properties of mouse connexin30.2 expressed in the conduction system of the heart Circ. Res. 96 2005 1169 1177 (Pubitemid 40827692)
12. V.K. Verselis, C.S. Ginter, and T.A. Bargiello Opposite voltage gating polarities of two closely related connexins Nature 368 1994 348 351 (Pubitemid 24104271)
13. 2 sensitivity of voltage gating with D3N mutation of Cx50 Am. J. Physiol. Cell Physiol. 288 2005 C1381 C1389
14. M. Srinivas, and J. Kronengold V.K. Verselis Correlative studies of gating in Cx46 and Cx50 hemichannels and gap junction channels Biophys. J. 88 2005 1725 1739 (Pubitemid 40976189)
15. L. Xin, X.Q. Gong, and D. Bai The role of amino terminus of mouse Cx50 in determining transjunctional voltage-dependent gating and unitary conductance Biophys. J. 99 2010 2077 2086
16. X.Q. Gong, and Q. Shao D.W. Laird Differential potency of dominant negative connexin43 mutants in oculodentodigital dysplasia J. Biol. Chem. 282 2007 19190 19202 (Pubitemid 47100175)
17. M. Srinivas, and R. Rozental D.C. Spray Functional properties of channels formed by the neuronal gap junction protein connexin36 J. Neurosci. 19 1999 9848 9855 (Pubitemid 30226658)
18. H. Musa, and E. Fenn R.D. Veenstra Amino terminal glutamate residues confer spermine sensitivity and affect voltage gating and channel conductance of rat connexin40 gap junctions J. Physiol. 557 2004 863 878 (Pubitemid 38961622)
19. A.P. Moreno, B. Eghbali, and D.C. Spray Connexin32 gap junction channels in stably transfected cells. Equilibrium and kinetic properties Biophys. J. 60 1991 1267 1277
20. R. Wilders, and H.J. Jongsma Limitations of the dual voltage clamp method in assaying conductance and kinetics of gap junction channels Biophys. J. 63 1992 942 953
21. J.B. Rubin, and V.K. Verselis T.A. Bargiello A domain substitution procedure and its use to analyze voltage dependence of homotypic gap junctions formed by connexins 26 and 32 Proc. Natl. Acad. Sci. USA 89 1992 3820 3824
22. D. Bai, and C. del Corsso D.C. Spray Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB) J. Pharmacol. Exp. Ther. 319 2006 1452 1458
23. F.J. Sigworth, and S.M. Sine Data transformations for improved display and fitting of single-channel dwell time histograms Biophys. J. 52 1987 1047 1054
24. + channel Cell 111 2002 231 239 (Pubitemid 35292443)
25. v channel J. Gen. Physiol. 122 2003 541 556
26. F. Bezanilla The voltage sensor in voltage-dependent ion channels Physiol. Rev. 80 2000 555 592 (Pubitemid 30164943)
27. V1 family potassium channel from Polyorchis penicillatus (Eschscholtz 1829) J. Exp. Biol. 211 2008 3442 3453
28. F.F. Bukauskas, and C. Elfgang R. Weingart Biophysical properties of gap junction channels formed by mouse connexin40 in induced pairs of transfected human HeLa cells Biophys. J. 68 1995 2289 2298
29. + channel Science 309 2005 897 903 (Pubitemid 41099919)
30. S. Oh, V.K. Verselis, and T.A. Bargiello Charges dispersed over the permeation pathway determine the charge selectivity and conductance of a Cx32 chimeric hemichannel J. Physiol. 586 2008 2445 2461 (Pubitemid 351689804)
31. P.E. Purnick, and S. Oh T.A. Bargiello Reversal of the gating polarity of gap junctions by negative charge substitutions in the N-terminus of connexin 32 Biophys. J. 79 2000 2403 2415
32. L. Dong, and X. Liu L. Ebihara Role of the N-terminus in permeability of chicken connexin45.6 gap junctional channels J. Physiol. 576 2006 787 799 (Pubitemid 44637883)
33. J.J. Tong, and X. Liu L. Ebihara Exchange of gating properties between rat cx46 and chicken cx45.6 Biophys. J. 87 2004 2397 2406 (Pubitemid 41071338)
34. J.J. Tong, and L. Ebihara Structural determinants for the differences in voltage gating of chicken Cx56 and Cx45.6 gap-junctional hemichannels Biophys. J. 91 2006 2142 2154 (Pubitemid 44352397)
35. S. Maeda, and S. Nakagawa T. Tsukihara Structure of the connexin 26 gap junction channel at 3.5 resolution Nature 458 2009 597 602
36. M. Srinivas, and M. Costa D.C. Spray Voltage dependence of macroscopic and unitary currents of gap junction channels formed by mouse connexin50 expressed in rat neuroblastoma cells J. Physiol. 517 1999 673 689 (Pubitemid 29306455)